T-CLEARE: a pilot community-driven tissue clearing protocol repository [PDF]
Frontiers in Bioengineering and BiotechnologySelecting and implementing a tissue clearing protocol is challenging. Established more than 100 years ago, tissue clearing is still a rapidly evolving field of research.
Kurt R. Weiss +13 more
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A guidebook for DISCO tissue clearing [PDF]
Molecular Systems Biology, 2021 Histological analysis of biological tissues by mechanical sectioning is significantly time‐consuming and error‐prone due to loss of important information during sample slicing.
Muge Molbay +4 more
doaj +7 more sources
Optimization of the optical transparency of bones by PACT-based passive tissue clearing [PDF]
Experimental and Molecular Medicine, 2023 Recent developments in tissue clearing methods such as the passive clearing technique (PACT) have allowed three-dimensional analysis of biological structures in whole, intact tissues, thereby providing a greater understanding of spatial relationships and
Byung-Ho Jin +9 more
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Compatibility of fast tissue clearing with targeted fluorescence imaging and pathology analysis using clinical head and neck cancer biopsies [PDF]
Scientific ReportsComplete tumor removal is crucial for successful surgical oncological outcomes. Targeted fluorescence imaging facilitates differentiation between healthy and cancerous tissue, enabling intraoperative margin assessment.
Ali Fakhrolmobasheri +7 more
doaj +2 more sources
3D visualization of uterus and ovary: tissue clearing techniques and biomedical applications [PDF]
Frontiers in Bioengineering and BiotechnologyRecent advancements in tissue clearing and three-dimensional (3D) visualization technologies have enabled subcellular-level examination of entire organs, particularly in complex structures such as the ovary and uterus. Traditional histological approaches
Qiqi Liu +6 more
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Rapid sonication-assisted whole tissue clearing and immunostaining [PDF]
Scientific ReportsHigh-resolution mapping of three-dimensional structures in biological tissues is essential for understanding various biological processes. However, the optical heterogeneity of these tissues, marked by varying optical properties, causes light scattering ...
Hoi Pan Harry Cheung +6 more
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Rapid and efficient optical tissue clearing for volumetric imaging of the intact and injured spinal cord in mice [PDF]
Frontiers in NeuroscienceTissue clearing and 3D imaging have emerged as powerful techniques to assess the cellular and tissue-level architecture of the spinal cord. With the rapidly increasing variety and complexity of optical tissue clearing techniques, there is a critical need
Andrew W. Buxton +5 more
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HyClear: A Novel Tissue Clearing Solution for One-Step Clearing of Microtissues [PDF]
Cells, 2022 3-D cell cultures are being increasingly used as in vitro models are capable of better mimicry of in vivo tissues, particularly in drug screenings where mass transfer limitations can affect the cancer biology and response to drugs.
S. Soroush Nasseri +3 more
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A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging [PDF]
Experimental and Molecular MedicineThe recent advancements in tissue-clearing techniques have opened new possibilities for non-invasive three-dimensional (3D) volumetric imaging of a wide range of biological specimens.
Kitae Kim +6 more
doaj +2 more sources
LiverClear: A versatile protocol for mouse liver tissue clearing [PDF]
STAR Protocols, 2022 Summary: Although there are numerous tissue clearing protocols, most are inadequate for clearing liver tissue. Here we present a flexible protocol for mouse liver tissue; we combine strategies from several previously published protocols for delipidation,
Laura M. Molina +8 more
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