Results 21 to 30 of about 177,664 (273)
Clearing for Deep Tissue Imaging [PDF]
Current Protocols in Cytometry, 2018 AbstractBiologic tissues are generally opaque due to optical properties that result in scattering and absorption of light. Preparation of tissues for optical microscopy often involves sectioning to a thickness of 50‐100 µm, the practical limits of light penetration and recovery.
Michael Muntifering +5 more
openaire +2 more sources
Frontiers in Bioengineering and Biotechnology, 2022 The advent of tissue clearing methods, in conjunction with novel high-resolution imaging techniques, has enabled the visualization of three-dimensional structures with unprecedented depth and detail.
Jiwon Woo +14 more
doaj +1 more source
Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping. [PDF]
, 2016 Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging ...
Chen, Harrison +12 more
core +2 more sources
Pharmaceutics, 2020 Visualizing biological events and states to resolve biological questions is challenging. Tissue clearing permits three-dimensional multicolor imaging. Here, we describe a pH-adjustable tissue clearing solution, Seebest (SEE Biological Events and States ...
Shintaro Fumoto +15 more
doaj +1 more source
Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy [PDF]
, 2011 In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering.
Clendenon, Sherry G. +4 more
core +1 more source
Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue [PDF]
Journal of Visualized Experiments, 2018 Adipose tissue plays a central role in energy homeostasis and thermoregulation. It is composed of different types of adipocytes, as well as adipocyte precursors, immune cells, fibroblasts, blood vessels, and nerve projections. Although the molecular control of cell type specification and how these cells interact have been increasingly delineated, a ...
Jingyi, Chi +3 more
openaire +2 more sources
Quantification of light attenuation in optically cleared mouse brains [PDF]
, 2015 Optical clearing, in combination with recently developed optical imaging techniques, enables visualization and acquisition of high-resolution, three-dimensional images of biological structures deep within the tissue. Many different approaches can be used
d'Esposito, A +4 more
core +1 more source
Neuroscience in the third dimension: shedding new light on the brain with tissue clearing
Molecular Brain, 2017 For centuries analyses of tissues have depended on sectioning methods. Recent developments of tissue clearing techniques have now opened a segway from studying tissues in 2 dimensions to 3 dimensions. This particular advantage echoes heavily in the field
Robin J. Vigouroux +2 more
doaj +1 more source
Cell, 2017 Tissue clearing has become an important tool for the investigation of biological systems in three dimensions. However, many pioneering techniques were based on serendipitous discoveries. Next-generation clearing methods have been (re)designed with a better understanding of the chemistry and physics required to equalize the refractive index throughout a
Douglas S, Richardson, Jeff W, Lichtman
openaire +2 more sources
Comprehensive Evaluation of Peripheral Nerve Regeneration in the Acute Healing Phase Using Tissue Clearing and Optical Microscopy in a Rodent Model [PDF]
, 2014 Peripheral nerve injury (PNI), a common injury in both the civilian and military arenas, is usually associated with high healthcare costs and with patients enduring slow recovery times, diminished quality of life, and potential long-term disability ...
Evans, Conor L. +7 more
core +3 more sources