Phage ϕC2 Mediates Transduction of Tn
mBio, 2013 In this work, we show that Clostridium difficile phage ϕC2 transduces erm(B), which confers erythromycin resistance, from a donor to a recipient strain at a frequency of 10−6 per PFU. The transductants were lysogenic for ϕC2 and contained the erm(B) gene
Shan Goh +5 more
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Genetic Modification of Sodalis Species by DNA Transduction
mSphere, 2021 A large number of economically important insects maintain intimate associations with maternally inherited endosymbiotic bacteria. Due to the inherent nature of these associations, insect endosymbionts cannot be usually isolated in pure culture or ...
Chelsea M. Keller +4 more
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Synthetic mechanoreceptor engineering: From genetic encoding to DNA nanotechnology-based reprogramming [PDF]
Mechanobiology in MedicinePrecise modulation of mechanoreceptor-mediated signal transduction is crucial for decoding cellular mechanotransduction mechanisms and programming cell fate.
Sihui Yang, Zhou Nie
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Increased in vivo transduction of AAV-9 cargo in Alport podocytes [version 1; peer review: 1 approved, 2 approved with reservations, 1 not approved] [PDF]
Wellcome Open ResearchBackground Alport syndrome is a rare genetic disorder characterized by progressive kidney disease, hearing loss, and eye abnormalities. It is caused by variants in the COL4A3, COL4A4 or COL4A5 genes, which disrupt the synthesis, secretion and assembly of
Emily Williams +8 more
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Repurposing Immunomodulatory Drugs to Combat Tuberculosis
Frontiers in Immunology, 2021 Tuberculosis (TB) is an infectious disease caused by an obligate intracellular pathogen, Mycobacterium tuberculosis (M.tb) and is responsible for the maximum number of deaths due to a single infectious agent.
Samreen Fatima +2 more
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Protocol for ex vivo competition and sequencing of mycobacterium isolated from infected guinea pigs
STAR Protocols, 2022 Summary: We describe steps for gDNA isolation from mycobacterium strains isolated from guinea pig lungs. We detail steps for infection of guinea pigs with Mycobacterium tuberculosis, followed by in vitro growth, gDNA isolation, and whole genome ...
Saba Naz +3 more
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Genetic Modification of Tumor-Infiltrating Lymphocytes via Retroviral Transduction
Frontiers in Immunology, 2021 Adoptive T cell therapy (ACT) holds great promise for cancer treatment. One approach, which has regained wide interest in recent years, employs antitumor T cells isolated from tumor lesions (“tumor-infiltrating lymphocytes” or TIL). It is now appreciated
Hadas Weinstein-Marom +11 more
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Frontiers in Bioengineering and Biotechnology, 2023 Genetic modification of pancreatic islet organoids, assembled in vitro prior to transplantation is an emerging alternative to direct in vivo genetic manipulations for a number of clinical and research applications.
Anna Voznesenskaya +2 more
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Nature Communications, 2021 It is commonly thought that horizontal transfer of most bacterial chromosomal genes is limited, in comparison with the frequent transfer of mobile genetic elements. Humphrey et al.
Suzanne Humphrey +6 more
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Host sirtuin 2 as an immunotherapeutic target against tuberculosis
eLife, 2020 Mycobacterium tuberculosis (Mtb) employs plethora of mechanisms to hijack the host defence machinery for its successful survival, proliferation and persistence.
Ashima Bhaskar +5 more
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