A Nontoxic Transduction Enhancer Enables Highly Efficient Lentiviral Transduction of Primary Murine T Cells and Hematopoietic Stem Cells [PDF]
Molecular Therapy - Methods and Clinical Development, 2018 Lentiviral vectors have emerged as an efficient, safe therapeutic tool for gene therapy based on hematopoietic stem cells (HSCs) or T cells. However, the monitoring of transduced cells in preclinical models remains challenging because of the inefficient ...
Emmanuelle Six +2 more
exaly +5 more sources
High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2 [PDF]
Molecular Therapy - Methods and Clinical Development, 2019 Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies.
Naoya Uchida +2 more
exaly +4 more sources
Hypoxia and HIF-1 inhibition enhance lentiviral transduction efficiency: a novel strategy for gene delivery optimization [PDF]
BMC BiotechnologyLentiviral vectors are widely used for stable gene delivery, but their transduction efficiency can be limited by suboptimal experimental conditions. Here, we investigated the role of oxygen concentration and hypoxia-inducible factor 1 (HIF-1) signaling ...
Qianyu Huo +10 more
doaj +3 more sources
Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing
VirusesTransduction of producer cells during lentiviral vector (LVV) production causes the loss of 70–90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the ...
Andres Lamsfus-Calle
exaly +4 more sources
Lentiviral Transduction of Neuronal Cells [PDF]
Methods in Molecular Biology, 2013 Here we describe a general method for the construction of a lentivirus vector using a specific example of the construction of a lentivirus containing the luciferase reporter gene under the control of two hypothetical promoters and derived HIV-1 based lentivirus expression vector pLVX-Puro.
Hassen Wollebo, Martyn K White
exaly +5 more sources
CD9 and folate receptor overexpression are not sufficient for VSV-G-independent lentiviral transduction [PDF]
PLoS ONE, 2022 Extracellular vesicles have become a research focus for their potential as therapeutic vehicles that carry cargo substances. Extracellular vesicles may origin from the endosomal compartment and share several characteristics with the envelope of ...
Cristina Bellotti +2 more
doaj +3 more sources
Systematic improvements in lentiviral transduction of primary human natural killer cells undergoing ex vivo expansion [PDF]
Molecular Therapy: Methods & Clinical Development, 2021 Transduction of primary human natural killer (NK) cells with lentiviral vectors has historically been challenging. We sought to evaluate multiple parameters to optimize lentiviral transduction of human peripheral blood NK cells being expanded to large ...
David S.J. Allan +6 more
doaj +2 more sources
Identification of a small molecule for enhancing lentiviral transduction of T cells [PDF]
Molecular Therapy: Methods & Clinical Development, 2023 Genetic modification of cells using viral vectors has shown huge therapeutic benefit in multiple diseases. However, inefficient transduction contributes to the high cost of these therapies.
Paulina Malach +10 more
doaj +2 more sources
Comparative analysis of CRISPR-Cas9, lentiviral transduction, and base editing for sickle cell disease in a murine model [PDF]
Blood Advances: Sickle cell disease (SCD) is a red blood cell disorder caused by a mutation in the β-globin gene, leading to sickle hemoglobin polymerization under low oxygen conditions.
Henna Butt +17 more
doaj +2 more sources
The effect of enhancers on the lentiviral transduction efficiency in the human RPE cells: Insights for advancing retinal gene therapies [PDF]
Biochemistry and Biophysics ReportsBackground: Viral vectors including lentiviruses (LV), adenoviruses (AV) and adeno-associated viruses (AAV) have been used as common vehicles for gene transfer in gene therapy of various human diseases.
Sajad Najafi +6 more
doaj +2 more sources