A Nontoxic Transduction Enhancer Enables Highly Efficient Lentiviral Transduction of Primary Murine T Cells and Hematopoietic Stem Cells [PDF]
Molecular Therapy - Methods and Clinical Development, 2018 Lentiviral vectors have emerged as an efficient, safe therapeutic tool for gene therapy based on hematopoietic stem cells (HSCs) or T cells. However, the monitoring of transduced cells in preclinical models remains challenging because of the inefficient ...
Emmanuelle Six +2 more
exaly +7 more sources
High-Efficiency Lentiviral Transduction of Human CD34+ Cells in High-Density Culture with Poloxamer and Prostaglandin E2 [PDF]
Molecular Therapy - Methods and Clinical Development, 2019 Hematopoietic stem cell (HSC) gene therapy is curative for various hereditary diseases; however, high-efficiency transduction in HSCs remains crucial to improve the prospects for hemoglobinopathies.
Naoya Uchida +2 more
exaly +5 more sources
Abolishing Retro-Transduction of Producer Cells in Lentiviral Vector Manufacturing [PDF]
VirusesTransduction of producer cells during lentiviral vector (LVV) production causes the loss of 70–90% of viable particles. This process is called retro-transduction and it is a consequence of the interaction between the LVV envelope protein, VSV-G, and the ...
Andres Lamsfus-Calle
exaly +5 more sources
Systematic improvements in lentiviral transduction of primary human natural killer cells undergoing ex vivo expansion [PDF]
Molecular Therapy: Methods & Clinical Development, 2021 Transduction of primary human natural killer (NK) cells with lentiviral vectors has historically been challenging. We sought to evaluate multiple parameters to optimize lentiviral transduction of human peripheral blood NK cells being expanded to large ...
David S.J. Allan +6 more
doaj +3 more sources
Improving Lentiviral Transduction of Human Adipose-Derived Mesenchymal Stem Cells. [PDF]
Hum Gene Ther, 2022 Lentiviral transduction of human mesenchymal stem cells (MSCs) induces long-term transgene expression and holds great promise for multiple gene therapy applications. Polybrene is the most commonly used reagent to improve viral gene transfer efficiency in
Collon K +7 more
europepmc +5 more sources
Identification of a small molecule for enhancing lentiviral transduction of T cells [PDF]
Molecular Therapy - Methods and Clinical Development, 2023 Genetic modification of cells using viral vectors has shown huge therapeutic benefit in multiple diseases. However, inefficient transduction contributes to the high cost of these therapies.
Paulina Malach +10 more
exaly +4 more sources
Rosuvastatin Enhances VSV-G Lentiviral Transduction of NK Cells via Upregulation of the Low-Density Lipoprotein Receptor [PDF]
Molecular Therapy - Methods and Clinical Development, 2020 Adoptive natural killer (NK) cell therapy is attaining promising clinical outcomes in recent years, but improvements are needed. Genetic modification of NK cells with a tumor antigen-specific receptor on their surface coupled to intracellular signaling ...
Ying Gong +2 more
exaly +3 more sources
Hypoxia and HIF-1 inhibition enhance lentiviral transduction efficiency: a novel strategy for gene delivery optimization [PDF]
BMC BiotechnologyLentiviral vectors are widely used for stable gene delivery, but their transduction efficiency can be limited by suboptimal experimental conditions. Here, we investigated the role of oxygen concentration and hypoxia-inducible factor 1 (HIF-1) signaling ...
Qianyu Huo +10 more
doaj +3 more sources
Cell Stem Cell, 2018 Summary Innate immune factors may restrict hematopoietic stem cell (HSC) genetic engineering and contribute to broad individual variability in gene therapy outcomes.
Bernhard Gentner +2 more
exaly +3 more sources
A protocol for lentiviral transduction and downstream analysis of intestinal organoids. [PDF]
J Vis Exp, 2015 Intestinal crypt-villus structures termed organoids, can be kept in sustained culture three dimensionally when supplemented with the appropriate growth factors. Since organoids are highly similar to the original tissue in terms of homeostatic stem cell differentiation, cell polarity and presence of all terminally differentiated cell types known to the ...
Van Lidth de Jeude JF +4 more
europepmc +5 more sources