Results 11 to 20 of about 247,262 (289)
Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors [PDF]
Journal of Biological Engineering, 2009 Background Lentiviral vectors with broad tropism are one of the most promising gene delivery systems capable of efficiently delivering genes of interest into both dividing and non-dividing cells while maintaining long-term transgene expression.
Wang Pin, Joo Kye-Il, Lei Yuning
doaj +2 more sources
A protocol for lentiviral transduction and downstream analysis of intestinal organoids. [PDF]
J Vis Exp, 2015 Intestinal crypt-villus structures termed organoids, can be kept in sustained culture three dimensionally when supplemented with the appropriate growth factors. Since organoids are highly similar to the original tissue in terms of homeostatic stem cell differentiation, cell polarity and presence of all terminally differentiated cell types known to the ...
Van Lidth de Jeude JF +4 more
europepmc +4 more sources
Transduction Patterns of Pseudotyped Lentiviral Vectors in the Nervous System
Molecular Therapy, 2004 We have developed a non-primate-based lentiviral vector based on the equine infectious anemia virus (EIAV) for efficient gene transfer to the central and peripheral nervous systems. Previously we have demonstrated that pseudotyping lentiviral vectors with the rabies virus glycoprotein confers retrograde axonal transport to these vectors. In the present
Wong, LF +7 more
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Molecular Therapy - Methods and Clinical Development, 2018 Lentiviral vector (LVV)-mediated transduction of human CD34+ hematopoietic stem and progenitor cells (HSPCs) holds tremendous promise for the treatment of monogenic hematological diseases.
Olivier Negre, Philip Gregory
exaly +3 more sources
A Scalable Lentiviral Workflow for Laboratory-Scale Generation of BCMA/GPRC5D Co-Transduced CAR-T Cells in Multiple Myeloma [PDF]
Current Issues in Molecular BiologyEfficient and reproducible lentiviral vector production and T-cell transduction remain important technical challenges in CAR-T (Chimeric Antigen Receptor T-cell) cell manufacturing.
Ewa Nowak +8 more
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Improving Lentiviral Transduction of Human Adipose-Derived Mesenchymal Stem Cells. [PDF]
Hum Gene Ther, 2022 Lentiviral transduction of human mesenchymal stem cells (MSCs) induces long-term transgene expression and holds great promise for multiple gene therapy applications. Polybrene is the most commonly used reagent to improve viral gene transfer efficiency in
Collon K +7 more
europepmc +2 more sources
Rosuvastatin Enhances VSV-G Lentiviral Transduction of NK Cells via Upregulation of the Low-Density Lipoprotein Receptor [PDF]
Molecular Therapy - Methods and Clinical Development, 2020 Ying Gong +2 more
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Process for an efficient lentiviral cell transduction [PDF]
Biology Methods and Protocols, 2020 Abstract The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause ...
Anna Chiara Pirona +3 more
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Journal of Translational Medicine, 2020 Background Adoptive transfer of engineered immune cells is a promising strategy for cancer treatment. However, low transduction efficiency particularly when large payload lentiviral vectors are used on primary T cells is a limitation for the development ...
Lingyu Li +13 more
doaj +1 more source
Lentiviral manipulation of gene expression in human adult and embryonic stem cells [PDF]
, 2006 Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery.
Joanne Crossley +11 more
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