Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors [PDF]
Journal of Biological Engineering, 2009 Background Lentiviral vectors with broad tropism are one of the most promising gene delivery systems capable of efficiently delivering genes of interest into both dividing and non-dividing cells while maintaining long-term transgene expression.
Wang Pin, Joo Kye-Il, Lei Yuning
doaj +2 more sources
Evaluation of transduction efficiency in macrophage colony-stimulating factor differentiated human macrophages using HIV-1 based lentiviral vectors [PDF]
BMC Biotechnology, 2011 Background Monocyte-derived macrophages contribute to atherosclerotic plaque formation. Therefore, manipulating macrophage function could have significant therapeutic value.
McCoy J Philip +3 more
doaj +2 more sources
Transduction Patterns of Pseudotyped Lentiviral Vectors in the Nervous System
Molecular Therapy, 2004 We have developed a non-primate-based lentiviral vector based on the equine infectious anemia virus (EIAV) for efficient gene transfer to the central and peripheral nervous systems. Previously we have demonstrated that pseudotyping lentiviral vectors with the rabies virus glycoprotein confers retrograde axonal transport to these vectors. In the present
Wong, LF +7 more
openaire +4 more sources
Efficient Transduction of T-Lymphocytes by Lentiviral Particles in Oncoimmunological Studies
Клиническая онкогематология, 2020 Aim. To compare different methods of lentivirus concentration in order to select the best way of providing high-level transduction for generating laboratory CAR-T cells. Materials & Methods. Concentration of lentiviral supernatant was carried out by 4
Ekaterina Konstantinovna Zaikova +5 more
doaj +2 more sources
Reduction of liver macrophage transduction by pseudotyping lentiviral vectors with a fusion envelope from
BMC Biotechnology, 2009 Background Lentiviral vectors are well suited for gene therapy because they can mediate long-term expression in both dividing and nondividing cells. However, lentiviral vectors seem less suitable for liver gene therapy because systemically administered ...
Hiralall Johan K +4 more
doaj +2 more sources
A Scalable Lentiviral Workflow for Laboratory-Scale Generation of BCMA/GPRC5D Co-Transduced CAR-T Cells in Multiple Myeloma [PDF]
Current Issues in Molecular BiologyEfficient and reproducible lentiviral vector production and T-cell transduction remain important technical challenges in CAR-T (Chimeric Antigen Receptor T-cell) cell manufacturing.
Ewa Nowak +8 more
doaj +2 more sources
Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells [PDF]
Retrovirology, 2004 Background Dendritic cells (DCs) are professional antigen-presenting cells that play important roles during human immunodeficiency virus type 1 (HIV-1) infection.
Chang Lung-Ji, He Jin, Chen Xiaochuan
doaj +2 more sources
Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4
, 2019 Lentiviral systems have proven advantageous in the delivery and long-term integration of gene sequences into the genome of several cell types in vitro, in vivo, as well as in clinical trials. Here we detail the protocols involved in the molecular cloning of ADAMTSL2 and ADAMTSL4 into the human immunodeficiency virus (HIV)-derived pCDH lentiviral system.
Singh, Mukti +2 more
openaire +4 more sources
Improving Lentiviral Transduction of Human Adipose-Derived Mesenchymal Stem Cells. [PDF]
Hum Gene Ther, 2022 Lentiviral transduction of human mesenchymal stem cells (MSCs) induces long-term transgene expression and holds great promise for multiple gene therapy applications. Polybrene is the most commonly used reagent to improve viral gene transfer efficiency in
Collon K +7 more
europepmc +2 more sources
Rosuvastatin Enhances VSV-G Lentiviral Transduction of NK Cells via Upregulation of the Low-Density Lipoprotein Receptor [PDF]
Molecular Therapy - Methods and Clinical Development, 2020 Ying Gong +2 more
exaly +2 more sources