ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactions [PDF]
PLoS Pathog, 2021 SARS-CoV-2 uses the human ACE2 (hACE2) receptor for cell attachment and entry, with mouse ACE2 (mACE2) unable to support infection. Herein we describe an ACE2-lentivirus system and illustrate its utility for in vitro and in vivo SARS-CoV-2 infection ...
Rawle DJ +6 more
europepmc +3 more sources
Molecular Therapy - Methods and Clinical Development, 2018 Lentiviral vector (LVV)-mediated transduction of human CD34+ hematopoietic stem and progenitor cells (HSPCs) holds tremendous promise for the treatment of monogenic hematological diseases.
Olivier Negre, Philip Gregory
exaly +4 more sources
DEAE-Dextran Enhances the Lentiviral Transduction of Primary Human Mesenchymal Stromal Cells from All Major Tissue Sources Without Affecting Their Proliferation and Phenotype. [PDF]
Mol Biotechnol, 2023 Genetic engineering of mesenchymal stromal cells (MSCs) is a tool widely used to explore MSC properties in vitro and in vivo. Lentiviral infection with the use of polycations as an adjuvant is a method that is commonly used to generate stably transduced ...
Amadeo F, Hanson V, Murray P, Taylor A.
europepmc +2 more sources
Cellular Proteo-Transcriptomic Changes in the Immediate Early-Phase of Lentiviral Transduction. [PDF]
Microorganisms, 2021 Lentivirus-based vectors derived from human immunodeficiency viruses type 1 and 2 (HIV-1 and 2) are widely used tools in research and may also be utilized in clinical settings.
Linkner TR +9 more
europepmc +2 more sources
Lentiviral transduction facilitates RNA interference in the nematode parasite Nippostrongylus brasiliensis. [PDF]
PLoS Pathog, 2021 Animal-parasitic nematodes have thus far been largely refractory to genetic manipulation, and methods employed to effect RNA interference (RNAi) have been ineffective or inconsistent in most cases. We describe here a new approach for genetic manipulation
Hagen J, Sarkies P, Selkirk ME.
europepmc +2 more sources
Efficient lentiviral transduction method to gene modify cord blood CD8<sup>+</sup> T cells for cancer therapy applications. [PDF]
Mol Ther Methods Clin Dev, 2021 Adoptive T cell therapy utilizing tumor-specific autologous T cells has shown promising results for cancer treatment. However, the limited numbers of autologous tumor-associated antigen (TAA)-specific T cells and the functional aberrancies, due to ...
Lo Presti V +7 more
europepmc +2 more sources
CD9 and folate receptor overexpression are not sufficient for VSV-G-independent lentiviral transduction [PDF]
PLoS ONE, 2022 Extracellular vesicles have become a research focus for their potential as therapeutic vehicles that carry cargo substances. Extracellular vesicles may origin from the endosomal compartment and share several characteristics with the envelope of ...
Cristina Bellotti +2 more
doaj +3 more sources
Process for an efficient lentiviral cell transduction [PDF]
Biology Methods and Protocols, 2020 The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction.
A. Pirona +3 more
semanticscholar +3 more sources
Biotechnology Reports (Amsterdam, Netherlands), 2023 Highlights • Lentiviral vectors have a limited half-life, and in order to reach the target, the distance between the target cell and the vector should be minimized.• Microfluidic systems provide a micro-scaled environment in which lentiviral vectors have
Seyed Esmaeil Ahmadi +2 more
exaly +2 more sources
Cell-based artificial APC resistant to lentiviral transduction for efficient generation of CAR-T cells from various cell sources. [PDF]
J Immunother Cancer, 2020 Background Adoptive cell therapy with chimeric antigen receptor T cells (CAR-T) has become a standard treatment for patients with certain aggressive B cell malignancies and holds promise to improve the care of patients suffering from numerous other ...
Schmidts A +17 more
europepmc +2 more sources