Results 21 to 30 of about 260,635 (281)

Process for an efficient lentiviral cell transduction [PDF]

Biology Methods and Protocols, 2020
Abstract The combination of lentiviruses with techniques such as CRISPR-Cas9 has resulted in efficient and precise processes for targeted genome modification. An often-limiting aspect, however, is the efficiency of cell transduction. Low efficiencies with particular cell types and/or the high complexity of lentiviral libraries can cause ...
Anna Chiara Pirona, Risky Oktriani, Michael Boettcher, Jörg D Hoheisel   +3 more
openaire   +2 more sources

Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKKɛ complex inhibitor BX795

Journal of Translational Medicine, 2020
Background Adoptive transfer of engineered immune cells is a promising strategy for cancer treatment. However, low transduction efficiency particularly when large payload lentiviral vectors are used on primary T cells is a limitation for the development ...
Lingyu Li, Yuan Gao, Richa Srivastava, Wei Wang, Qinghui Xiong, Zhiming Fang, Alejandra Pelayo, Carolyn Denson, Angshumala Goswami, Rona Harari-Steinfeld, Zhifen Yang, Lihong Weng, Lei Stanley Qi, Francesco M. Marincola   +13 more
doaj   +1 more source

Highly efficient ex vivo lentiviral transduction of primary human pancreatic exocrine cells. [PDF]

Sci Rep, 2019
The lack of efficient gene transfer methods into primary human pancreatic exocrine cells hampers studies on the plasticity of these cells and their possible role in beta cell regeneration.
Balak JRA, de Graaf N, Zaldumbide A, Rabelink TJ, Hoeben RC, de Koning EJP, Carlotti F.   +6 more
europepmc   +2 more sources

Lentiviral Transduction of Neuronal Cells [PDF]

, 2013
Here we describe a general method for the construction of a lentivirus vector using a specific example of the construction of a lentivirus containing the luciferase reporter gene under the control of two hypothetical promoters and derived HIV-1 based lentivirus expression vector pLVX-Puro.
Hassen S, Wollebo, Baheru, Woldemichaele, Martyn K, White   +2 more
openaire   +2 more sources

Lentiviral-mediated overexpression of ESR1 and ESR2.

, 2023
(A) HUVEC and HDMVEC tolerance of lentiviral transduction was measured by fluorescence 72 hours after lenti-mCherry transduction. (B) ESR1 and (C) ESR2 expression was measured by RT-qPCR and normalized to lenti-mCherry samples (N = 4).
Homa K. Ahmadzia (6087572), Yaqiu Sang (4814460), Nicholas L. Smith (8174139), Emma G. Bouck (15322597), Alisa S. Wolberg (14796826), William O. Osburn (3780055), Paula Reventun (15322600), Charles J. Lowenstein (12568953), Marios Arvanitis (528431)   +8 more
core   +1 more source

Lentiviral manipulation of gene expression in human adult and embryonic stem cells [PDF]

, 2006
Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery.
Joanne Crossley, Crossley, J., Andrew Godfrey, Takeuchi, Y., Clements, M.O., Boshoff, C., Godfrey, A., Mark O. Clements, Sam J. Wilson, Wilson, S.J., Yasu Takeuchi, Chris Boshoff   +11 more
core   +1 more source

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors [PDF]

, 2004
BACKGROUND: Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the
Hermens, Wim T J M C, Lieberman, A., Eggers, R., Verhaagen, J., Sablitzky, Fred, Chakravarthy, Sridhara, Zhang, Jing Ying, Zhang, J.Y., Hermens, Wim TJMC, Zhang, Jing, Verhaagen, J, Ahmed, Bushra, Anderson, P.N., Eggers, R, Eggers, Ruben, Levelt, Christiaan, Chakravarthy, S, Levelt, C., Ahmed, BY, Verhaagen, Joost, Lieberman, A.R., Hermens, WTJMC, Lieberman, AR, Anderson, Patrick N., Ahmed, Bushra Y, Hermens, W.T., Niclou, SP, Anderson, PN, NICLOU, Simone P., Lieberman, A R, Zhang, JY, Anderson, Patrick N, Sablitzky, F., Levelt, C, Sablitzky, F, Niclou, S.P., Chakravarthy, S., Ahmed, B.Y.   +37 more
core   +1 more source

Gene delivery to pancreatic exocrine cells in vivo and in vitro

BMC Biotechnology, 2012
Background Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested
Houbracken Isabelle, Baeyens Luc, Ravassard Philippe, Heimberg Harry, Bouwens Luc   +4 more
doaj   +1 more source

Remission of invasive, cancer stem-like glioblastoma xenografts using lentiviral vector-mediated suicide gene therapy [PDF]

, 2009
Background: Glioblastoma is the most frequent and most malignant primary brain tumor with a poor prognosis. The translation of therapeutic strategies for glioblastoma from the experimental phase into the clinic has been limited by insufficient animal ...
Oleg Tsinkalovsky, Euskirchen Philipp, Tsanan Giroglou, Huszthy, Peter C., Giroglou Tsanan, Skaftnesmo, Kai Ove, Miletic, Hrvoje, Miletic Hrvoje, Peter C Huszthy, Kai Ove Skaftnesmo, Tsinkalovsky Oleg, von Laer Dorothee, Euskirchen, Philipp, Dorothee von Laer, Hrvoje Miletic, Laer, Dorothee von, Rolf Bjerkvig, Giroglou, Tsanan, Tsinkalovsky, Oleg, Huszthy Peter C., Skaftnesmo Kai Ove, Bjerkvig Rolf, Philipp Euskirchen, Bjerkvig, Rolf   +23 more
core   +2 more sources

An efficient method for the transduction of primary pediatric glioma neurospheres

MethodsX, 2018
Pediatric high grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are rare, but rapidly fatal malignancies of the central nervous system (CNS), and the leading cause of cancer-related death in children.
Michaël H. Meel, Dennis S. Metselaar, Piotr Waranecki, Gertjan J.L. Kaspers, Esther Hulleman   +4 more
doaj   +1 more source