Results 21 to 30 of about 247,262 (289)

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors [PDF]

, 2004
BACKGROUND: Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the
Hermens, Wim T J M C, Lieberman, A., Eggers, R., Verhaagen, J., Sablitzky, Fred, Chakravarthy, Sridhara, Zhang, Jing Ying, Zhang, J.Y., Hermens, Wim TJMC, Zhang, Jing, Verhaagen, J, Ahmed, Bushra, Anderson, P.N., Eggers, R, Eggers, Ruben, Levelt, Christiaan, Chakravarthy, S, Levelt, C., Ahmed, BY, Verhaagen, Joost, Lieberman, A.R., Hermens, WTJMC, Lieberman, AR, Niclou, Simone P, Anderson, Patrick N., Ahmed, Bushra Y, Hermens, W.T., Niclou, SP, Anderson, PN, NICLOU, Simone P., Lieberman, A R, Zhang, JY, Anderson, Patrick N, Sablitzky, F., Levelt, C, Sablitzky, F, Niclou, S.P., Lieberman, A Robert, Chakravarthy, S., Ahmed, B.Y.   +39 more
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Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison [PDF]

, 2008
Cross-neutralization between rabies virus (RABV) and two European bat lyssaviruses (EBLV-1 and -2) was analysed using lentiviral pseudotypes as antigen vectors.
Temperton, Nigel J., McElhinney, Lorraine M., Fooks, A.R., Marston, Denise A., Weiss, R.A., Marston, D.A., McElhinney, L.M., Temperton, N.J., Fooks, Anthony R., Wright, Edward, Fooks, Anthiny R., Wright, E., Weiss, Robin A.   +12 more
core   +1 more source

Gene delivery to pancreatic exocrine cells in vivo and in vitro

BMC Biotechnology, 2012
Background Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested
Houbracken Isabelle, Baeyens Luc, Ravassard Philippe, Heimberg Harry, Bouwens Luc   +4 more
doaj   +1 more source

An efficient method for the transduction of primary pediatric glioma neurospheres

MethodsX, 2018
Pediatric high grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are rare, but rapidly fatal malignancies of the central nervous system (CNS), and the leading cause of cancer-related death in children.
Michaël H. Meel, Dennis S. Metselaar, Piotr Waranecki, Gertjan J.L. Kaspers, Esther Hulleman   +4 more
doaj   +1 more source

Remission of invasive, cancer stem-like glioblastoma xenografts using lentiviral vector-mediated suicide gene therapy [PDF]

, 2009
Background: Glioblastoma is the most frequent and most malignant primary brain tumor with a poor prognosis. The translation of therapeutic strategies for glioblastoma from the experimental phase into the clinic has been limited by insufficient animal ...
Oleg Tsinkalovsky, Euskirchen Philipp, Tsanan Giroglou, Huszthy, Peter C., Giroglou Tsanan, Skaftnesmo, Kai Ove, Miletic, Hrvoje, Miletic Hrvoje, Peter C Huszthy, Kai Ove Skaftnesmo, Tsinkalovsky Oleg, von Laer Dorothee, Euskirchen, Philipp, Dorothee von Laer, Hrvoje Miletic, Laer, Dorothee von, Rolf Bjerkvig, Giroglou, Tsanan, Tsinkalovsky, Oleg, Huszthy Peter C., Skaftnesmo Kai Ove, Bjerkvig Rolf, Philipp Euskirchen, Bjerkvig, Rolf   +23 more
core   +2 more sources

Lentiviral-mediated overexpression of ESR1 and ESR2.

, 2023
(A) HUVEC and HDMVEC tolerance of lentiviral transduction was measured by fluorescence 72 hours after lenti-mCherry transduction. (B) ESR1 and (C) ESR2 expression was measured by RT-qPCR and normalized to lenti-mCherry samples (N = 4).
Homa K. Ahmadzia (6087572), Yaqiu Sang (4814460), Nicholas L. Smith (8174139), Emma G. Bouck (15322597), Alisa S. Wolberg (14796826), William O. Osburn (3780055), Paula Reventun (15322600), Charles J. Lowenstein (12568953), Marios Arvanitis (528431)   +8 more
core   +1 more source

Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

Molecular Therapy: Methods & Clinical Development, 2016
Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in ...
Michele E Murphy, Chintan D Vin, Megan M Slough, Wayne R Gombotz, Brenna Kelley-Clarke   +4 more
doaj   +1 more source

Modular Lentiviral Vectors for Highly Efficient Transgene Expression in Resting Immune Cells

Viruses, 2021
Gene/cell therapies are promising strategies for the many presently incurable diseases. A key step in this process is the efficient delivery of genes and gene-editing enzymes to many cell types that may be resistant to lentiviral vector transduction ...
Christina Fichter, Anupriya Aggarwal, Andrew Kam Ho Wong, Samantha McAllery, Vennila Mathivanan, Bailey Hao, Hugh MacRae, Melissa J. Churchill, Paul R. Gorry, Michael Roche, Lachlan R. Gray, Stuart Turville   +11 more
doaj   +1 more source

In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]

, 2010
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Candrian, Christian, Gianni-Barrera, Roberto, Juelke, Henriette, Martin, Ivan, Jaquiery, Claude, Acharya, Chitrangada, Barbero, Andrea, Miot, Sylvie, Pelttari, Karoliina, Mainil-Varlet, Pierre   +9 more
core   +2 more sources

Anti-Apoptotic Effects of Lentiviral Vector Transduction Promote Increased Rituximab Tolerance in Cancerous B-Cells. [PDF]

PLoS ONE, 2016
Diffuse large B-cell lymphoma (DLBCL) is characterized by great genetic and clinical heterogeneity which complicates prognostic prediction and influences treatment efficacy. The most common regimen, R-CHOP, consists of a combination of anthracycline- and
Benyamin Ranjbar, Louise Bechmann Krogh, Maria Bach Laursen, Maria Nascimento Primo, Sara Correia Marques, Karen Dybkær, Jacob Giehm Mikkelsen   +6 more
doaj   +1 more source