Results 21 to 30 of about 368,855 (303)

Optimized protocols for γδ T cell expansion and lentiviral transduction. [PDF]

Mol Med Rep, 2019
γδ T cells are a subset of unconventional T cells that serve a critical role in infectious diseases and various types of cancer. Cell therapy with genetically-modified γδ T cells is regarded as a promising tool for tumor treatment.
Wang RN, Wen Q, He WT, Yang JH, Zhou CY, Xiong WJ, Ma L.   +6 more
europepmc   +2 more sources

Purification of Human CD34⁺CD90⁺ HSCs Reduces Target Cell Population and Improves Lentiviral Transduction for Gene Therapy. [PDF]

Mol Ther Methods Clin Dev, 2020
Hematopoietic stem cell (HSC) gene therapy has the potential to cure many genetic, malignant, and infectious diseases. We have shown in a nonhuman primate gene therapy and transplantation model that the CD34+CD90+ cell fraction was exclusively ...
Radtke S, Pande D, Cui M, Perez AM, Chan YY, Enstrom M, Schmuck S, Berger A, Eunson T, Adair JE, Kiem HP.   +10 more
europepmc   +2 more sources

Comparative analysis of CRISPR-Cas9, lentiviral transduction, and base editing for sickle cell disease in a murine model [PDF]

Blood Advances
: Sickle cell disease (SCD) is a red blood cell disorder caused by a mutation in the β-globin gene, leading to sickle hemoglobin polymerization under low oxygen conditions.
Henna Butt, Shruti Sathish, Evan London, Anh Le, Quan Li, Bjorg Gudmundsdottir, Duck-Yeon Lee, Emma V. Burke, Bradley P. Yates, David R. Liu, Matthew Hsieh, Alexis Leonard, William A. Eaton, Naoya Uchida, Francis J. Pierciey, Jr., Gregory A. Newby, John F. Tisdale, Selami Demirci   +17 more
doaj   +2 more sources

Highly efficient ex vivo lentiviral transduction of primary human pancreatic exocrine cells. [PDF]

Sci Rep, 2019
The lack of efficient gene transfer methods into primary human pancreatic exocrine cells hampers studies on the plasticity of these cells and their possible role in beta cell regeneration.
Balak JRA, de Graaf N, Zaldumbide A, Rabelink TJ, Hoeben RC, de Koning EJP, Carlotti F.   +6 more
europepmc   +2 more sources

Lentiviral transduction of mammalian cells for fast, scalable and high-level production of soluble and membrane proteins. [PDF]

Nat Protoc, 2018
Structural, biochemical and biophysical studies of eukaryotic soluble and membrane proteins require their production in milligram quantities. Although large-scale protein expression strategies based on transient or stable transfection of mammalian cells ...
Elegheert J, Behiels E, Bishop B, Scott S, Woolley RE, Griffiths SC, Byrne EFX, Chang VT, Stuart DI, Jones EY, Siebold C, Aricescu AR.   +11 more
europepmc   +2 more sources

The effect of enhancers on the lentiviral transduction efficiency in the human RPE cells: Insights for advancing retinal gene therapies [PDF]

Biochemistry and Biophysics Reports
Background: Viral vectors including lentiviruses (LV), adenoviruses (AV) and adeno-associated viruses (AAV) have been used as common vehicles for gene transfer in gene therapy of various human diseases.
Sajad Najafi, Azam Rahimpour, Hamid Ahmadieh, Mozhgan Rezaei Kanavi, Maryam Maleki Tehrani, Fatemeh Suri, Javad Ranjbari   +6 more
doaj   +2 more sources

Lentiviral transduction of neuronal cells. [PDF]

Methods Mol Biol, 2013
Here we describe a general method for the construction of a lentivirus vector using a specific example of the construction of a lentivirus containing the luciferase reporter gene under the control of two hypothetical promoters and derived HIV-1 based lentivirus expression vector pLVX-Puro.
Wollebo HS, Woldemichaele B, White MK.
europepmc   +4 more sources

Concise review on optimized methods in production and transduction of lentiviral vectors in order to facilitate immunotherapy and gene therapy

Biomedicine and Pharmacotherapy, 2020
Lentiviral vectors (LVs) have provided an efficient way to integrate our gene of interest into eukaryote cells. Human immunodeficiency virus (HIV)-derived LVs have been vastly studied to become an invaluable asset in gene delivery.
Hadi Esmaeili Gourvarchin Galeh, Ali Jafari, Ruhollah Dorostkar   +2 more
exaly   +2 more sources

Auto‐transduction in lentiviral vector bioprocessing: A quantitative assessment and a novel inhibition strategy

Biotechnology and Bioengineering
Lentiviral vectors are highly efficient gene delivery vehicles used extensively in the rapidly growing field of cell and gene therapy. Demand for efficient, large‐scale, lentiviral vector bioprocessing is growing as more therapies reach late‐stage ...
Qasim Rafiq, Thomas Williams-Fegredo
exaly   +2 more sources

Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors [PDF]

Journal of Biological Engineering, 2009
Background Lentiviral vectors with broad tropism are one of the most promising gene delivery systems capable of efficiently delivering genes of interest into both dividing and non-dividing cells while maintaining long-term transgene expression.
Wang Pin, Joo Kye-Il, Lei Yuning
doaj   +2 more sources