Results 31 to 40 of about 368,855 (303)

Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs

Stem Cell Research, 2015
Understanding how to achieve efficient transduction of hematopoietic stem and progenitor cells (HSPCs), while preserving their long-term ability to self-reproduce, is key for applying lentiviral-based gene engineering methods.
Duo Li, Erika Schlaepfer, Annette Audigé, Mary-Aude Rochat, Sandra Ivic, Caitlin N. Knowlton, Baek Kim, Oliver T. Keppler, Roberto F. Speck   +8 more
doaj   +2 more sources

Transduction Patterns of Pseudotyped Lentiviral Vectors in the Nervous System [PDF]

Molecular Therapy, 2004
We have developed a non-primate-based lentiviral vector based on the equine infectious anemia virus (EIAV) for efficient gene transfer to the central and peripheral nervous systems. Previously we have demonstrated that pseudotyping lentiviral vectors with the rabies virus glycoprotein confers retrograde axonal transport to these vectors. In the present
Wong, LF, Azzouz, M, Walmsley, LE, Askham, Z, Wilkes, FJ, Mitrophanous, KA, Kingsman, SM, Mazarakis, ND   +7 more
openaire   +4 more sources

Enhancing Lentiviral and Alpharetroviral Transduction of Human Hematopoietic Stem Cells for Clinical Application

Molecular Therapy - Methods and Clinical Development, 2019
Ex vivo retroviral gene transfer into CD34+ hematopoietic stem and progenitor cells (HSPCs) has demonstrated remarkable clinical success in gene therapy for monogenic hematopoietic disorders.
Karen Buckland, Alessia Cavazza, Adrian Thrasher   +2 more
exaly   +2 more sources

Vectofusin-1 Promotes RD114-TR-Pseudotyped Lentiviral Vector Transduction of Human HSPCs and T Lymphocytes

Molecular Therapy - Methods and Clinical Development, 2017
Ex vivo transduction of human CD34+ hematopoietic stem/progenitor cells (hCD34+ HSPCs) and T lymphocytes is a key process that requires high efficiency and low toxicity to achieve effective clinical results.
Anne Galy, David Fénard, Chiara Bovolenta   +2 more
exaly   +2 more sources

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells. [PDF]

J Vis Exp, 2018
Nanbakhsh A, Best B, Riese M, Rao S, Wang L, Medin J, Thakar MS, Malarkannan S.   +7 more
europepmc   +2 more sources

Lentiviral-mediated overexpression of ESR1 and ESR2. [PDF]

, 2023
(A) HUVEC and HDMVEC tolerance of lentiviral transduction was measured by fluorescence 72 hours after lenti-mCherry transduction. (B) ESR1 and (C) ESR2 expression was measured by RT-qPCR and normalized to lenti-mCherry samples (N = 4).
Homa K. Ahmadzia (6087572), Yaqiu Sang (4814460), Nicholas L. Smith (8174139), Emma G. Bouck (15322597), Alisa S. Wolberg (14796826), William O. Osburn (3780055), Paula Reventun (15322600), Charles J. Lowenstein (12568953), Marios Arvanitis (528431)   +8 more
core   +1 more source

Lentiviral delivery of combinatorial CAR/CRISPRi circuit into human primary T cells is enhanced by TBK1/IKKɛ complex inhibitor BX795

Journal of Translational Medicine, 2020
Background Adoptive transfer of engineered immune cells is a promising strategy for cancer treatment. However, low transduction efficiency particularly when large payload lentiviral vectors are used on primary T cells is a limitation for the development ...
Lingyu Li, Yuan Gao, Richa Srivastava, Wei Wang, Qinghui Xiong, Zhiming Fang, Alejandra Pelayo, Carolyn Denson, Angshumala Goswami, Rona Harari-Steinfeld, Zhifen Yang, Lihong Weng, Lei Stanley Qi, Francesco M. Marincola   +13 more
doaj   +1 more source

Lentiviral manipulation of gene expression in human adult and embryonic stem cells [PDF]

, 2006
Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery.
Joanne Crossley, Crossley, J., Andrew Godfrey, Takeuchi, Y., Clements, M.O., Boshoff, C., Godfrey, A., Mark O. Clements, Sam J. Wilson, Wilson, S.J., Yasu Takeuchi, Chris Boshoff   +11 more
core   +1 more source

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors [PDF]

, 2004
BACKGROUND: Inactivating genes in vivo is an important technique for establishing their function in the adult nervous system. Unfortunately, conventional knockout mice may suffer from several limitations including embryonic or perinatal lethality and the
Hermens, Wim T J M C, Lieberman, A., Eggers, R., Verhaagen, J., Sablitzky, Fred, Chakravarthy, Sridhara, Zhang, Jing Ying, Zhang, J.Y., Hermens, Wim TJMC, Zhang, Jing, Verhaagen, J, Ahmed, Bushra, Anderson, P.N., Eggers, R, Eggers, Ruben, Levelt, Christiaan, Chakravarthy, S, Levelt, C., Ahmed, BY, Verhaagen, Joost, Lieberman, A.R., Hermens, WTJMC, Lieberman, AR, Niclou, Simone P, Anderson, Patrick N., Ahmed, Bushra Y, Hermens, W.T., Niclou, SP, Anderson, PN, NICLOU, Simone P., Lieberman, A R, Zhang, JY, Anderson, Patrick N, Sablitzky, F., Levelt, C, Sablitzky, F, Niclou, S.P., Lieberman, A Robert, Chakravarthy, S., Ahmed, B.Y.   +39 more
core   +1 more source

Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison [PDF]

, 2008
Cross-neutralization between rabies virus (RABV) and two European bat lyssaviruses (EBLV-1 and -2) was analysed using lentiviral pseudotypes as antigen vectors.
Temperton, Nigel J., McElhinney, Lorraine M., Fooks, A.R., Marston, Denise A., Weiss, R.A., Marston, D.A., McElhinney, L.M., Temperton, N.J., Fooks, Anthony R., Wright, Edward, Fooks, Anthiny R., Wright, E., Weiss, Robin A.   +12 more
core   +1 more source