Results 31 to 40 of about 260,635 (281)
A protocol for lentiviral transduction and downstream analysis of intestinal organoids. [PDF]
J Vis Exp, 2015 Intestinal crypt-villus structures termed organoids, can be kept in sustained culture three dimensionally when supplemented with the appropriate growth factors. Since organoids are highly similar to the original tissue in terms of homeostatic stem cell differentiation, cell polarity and presence of all terminally differentiated cell types known to the ...
Van Lidth de Jeude JF +4 more
europepmc +4 more sources
Molecular Therapy: Methods & Clinical Development, 2016 Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in ...
Michele E Murphy +4 more
doaj +1 more source
Modular Lentiviral Vectors for Highly Efficient Transgene Expression in Resting Immune Cells
Viruses, 2021 Gene/cell therapies are promising strategies for the many presently incurable diseases. A key step in this process is the efficient delivery of genes and gene-editing enzymes to many cell types that may be resistant to lentiviral vector transduction ...
Christina Fichter +11 more
doaj +1 more source
Visualization of targeted transduction by engineered lentiviral vectors [PDF]
Gene Therapy, 2008 We have reported a method to target lentiviral vectors to specific cell types. This method requires the incorporation of two distinct molecules on the viral vector surface: one is an antibody that renders the targeting specificity for the engineered vector, and the other is a fusogenic protein that allows the engineered vector to enter the target cell.
K-I, Joo, P, Wang
openaire +2 more sources
Investigating antibody neutralization of lyssaviruses using lentiviral pseudotypes: a cross-species comparison [PDF]
, 2008 Cross-neutralization between rabies virus (RABV) and two European bat lyssaviruses (EBLV-1 and -2) was analysed using lentiviral pseudotypes as antigen vectors.
Temperton, Nigel J. +12 more
core +1 more source
Anti-Apoptotic Effects of Lentiviral Vector Transduction Promote Increased Rituximab Tolerance in Cancerous B-Cells. [PDF]
PLoS ONE, 2016 Diffuse large B-cell lymphoma (DLBCL) is characterized by great genetic and clinical heterogeneity which complicates prognostic prediction and influences treatment efficacy. The most common regimen, R-CHOP, consists of a combination of anthracycline- and
Benyamin Ranjbar +6 more
doaj +1 more source
Expression of a large coding sequence: Gene therapy vectors for Ataxia Telangiectasia
Scientific Reports, 2023 Ataxia telangiectasia is a monogenetic disorder caused by mutations in the ATM gene. Its encoded protein kinase ATM plays a fundamental role in DNA repair of double strand breaks (DSBs).
Tanja Hirch +6 more
doaj +1 more source
Driving DNA transposition by lentiviral protein transduction [PDF]
Mobile Genetic Elements, 2014 Gene vectors derived from DNA transposable elements have become powerful molecular tools in biomedical research and are slowly moving into the clinic as carriers of therapeutic genes. Conventional uses of DNA transposon-based gene vehicles rely on the intracellular production of the transposase protein from transfected nucleic acids.
Cai, Yujia, Mikkelsen, Jacob Giehm
openaire +2 more sources
JCI Insight, 2022 Lentiviral vector–based dendritic cell vaccines induce protective T cell responses against viral infection and cancer in animal models. In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of ...
Takuya Tada +3 more
doaj +1 more source