Results 41 to 50 of about 260,635 (281)
Stem Cell Research, 2015 Understanding how to achieve efficient transduction of hematopoietic stem and progenitor cells (HSPCs), while preserving their long-term ability to self-reproduce, is key for applying lentiviral-based gene engineering methods.
Duo Li +8 more
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Revista Colombiana de Ciencias Pecuarias, 2019 Background: Production of transgenic animals is still a low-efficiency biotechnology, and simple alternatives should be used to improve the rate of transgenic bovine production by nuclear transfer.
Alinne G. Curcio +5 more
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Avoiding lentiviral transduction culture induced MSC senescence [PDF]
Journal of Cellular and Molecular Medicine, 2009 Dear Editor: We read with interest the recent article by Bocker and colleagues in JCMM[1]. As noted by the authors, mesenchymal stem cells (MSC) enjoy considerable therapeutic potential in regenerative medicine, cell therapy for select genetic diseases and as immunomodulators [2, 3].
Pan, Yung-Wei, Kurre, Peter
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In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]
, 2010 We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Candrian, Christian +19 more
core +2 more sources
Effectiveness of gene delivery systems for pluripotent and differentiated cells
Molecular Therapy: Methods & Clinical Development, 2015 Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) assert a great future for the cardiovascular diseases, both to study them and to explore therapies. However, a comprehensive assessment of the viral vectors used to modify these
Kleopatra Rapti +6 more
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Molecular Oncology, EarlyView.This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi +6 more
wiley +1 more source
Generation of stably expressing IRF5 spliced isoform in Jurkat cells
Progress in Microbes and Molecular Biology, 2020 Lentiviral transduction enables the generation of gain-of-function of a targeted gene in mammalian cells. Single-cell cloning through limiting dilution can establish a population of cells with homogenous transgene expression for exploring protein ...
Ashwinder Kaur, Chee-Mun Fang
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Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells. [PDF]
PLoS ONE, 2015 The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes ...
Wojciech Witkowski +7 more
doaj +1 more source
Molecular Oncology, EarlyView.Partial inhibition of focal adhesion kinase (FAK) can paradoxically promote tumor growth, rather than simply producing a weaker antitumor effect than that observed with strong FAK suppression. In breast cancer and melanoma models, targeting p110δ PI3K, particularly in macrophages, counteracted these tumor‐promoting effects, highlighting the importance ...
Lydia Xenou +4 more
wiley +1 more source
Kinetics of lentiviral vector transduction in human CD34+ cells [PDF]
Experimental Hematology, 2016 Unlike cell lines, human hematopoietic stem cells (HSCs) are less efficiently transduced with HIV-1 vectors, potentially limiting this approach. To investigate which step (internalization, reverse transcription, nuclear transport, and integration) limits lentiviral transduction, we evaluated the kinetics of lentiviral transduction in human CD34 ...
Naoya, Uchida +5 more
openaire +2 more sources