Gene delivery to pancreatic exocrine cells
BMC Biotechnology, 2012 Background Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested
Houbracken Isabelle +4 more
doaj +1 more source
An efficient method for the transduction of primary pediatric glioma neurospheres
MethodsX, 2018 Pediatric high grade glioma (pHGG) and diffuse intrinsic pontine glioma (DIPG) are rare, but rapidly fatal malignancies of the central nervous system (CNS), and the leading cause of cancer-related death in children.
Michaël H. Meel +4 more
doaj +1 more source
Antibody‐Based CAR T Cells Produced by Lentiviral Transduction
Current Protocols in Immunology, 2020 One promising approach to treat hematologic malignancies is the usage of patient‐derived CAR T cells. There are continuous efforts to improve the function of these cells, to optimize their receptor, and to use them for the treatment of additional types ...
Sabrina Prommersberger +2 more
semanticscholar +1 more source
Visualization of targeted transduction by engineered lentiviral vectors [PDF]
Gene Therapy, 2008 We have reported a method to target lentiviral vectors to specific cell types. This method requires the incorporation of two distinct molecules on the viral vector surface: one is an antibody that renders the targeting specificity for the engineered vector, and the other is a fusogenic protein that allows the engineered vector to enter the target cell.
K-I, Joo, P, Wang
openaire +2 more sources
Remission of invasive, cancer stem-like glioblastoma xenografts using lentiviral vector-mediated suicide gene therapy [PDF]
, 2009 Background: Glioblastoma is the most frequent and most malignant primary brain tumor with a poor prognosis. The translation of therapeutic strategies for glioblastoma from the experimental phase into the clinic has been limited by insufficient animal ...
Oleg Tsinkalovsky +23 more
core +1 more source
Molecular Therapy: Methods & Clinical Development, 2016 Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in ...
Michele E Murphy +4 more
doaj +1 more source
Modular Lentiviral Vectors for Highly Efficient Transgene Expression in Resting Immune Cells
Viruses, 2021 Gene/cell therapies are promising strategies for the many presently incurable diseases. A key step in this process is the efficient delivery of genes and gene-editing enzymes to many cell types that may be resistant to lentiviral vector transduction ...
Christina Fichter +11 more
doaj +1 more source
Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk [PDF]
, 2012 We have previously shown that injury-induced neointima formation was rescued by adenoviral-Nogo-B gene delivery. Integrase-competent lentiviral vectors (ICLV) are efficient at gene delivery to vascular cells but present a risk of insertional mutagenesis.
Sessa, W.C. +10 more
core +1 more source
Anti-Apoptotic Effects of Lentiviral Vector Transduction Promote Increased Rituximab Tolerance in Cancerous B-Cells. [PDF]
PLoS ONE, 2016 Diffuse large B-cell lymphoma (DLBCL) is characterized by great genetic and clinical heterogeneity which complicates prognostic prediction and influences treatment efficacy. The most common regimen, R-CHOP, consists of a combination of anthracycline- and
Benyamin Ranjbar +6 more
doaj +1 more source
Lentiviral vector induces high-quality memory T cells via dendritic cells transduction
Communications Biology, 2021 We report a lentiviral vector harboring the human β2-microglobulin promoter, with predominant expression in immune cells and minimal proximal enhancers to improve vector safety.
Min-Wen Ku +7 more
semanticscholar +1 more source