Results 61 to 70 of about 368,855 (303)
Lentiviral vector and transduction procedures. [PDF]
, 2013 (A) Schematic diagram of Cell Genesys SIN lentiviral transfer vector pKC.MND.eGFP.neo. CMV 5′ LTR: modified cytomegalovirus 5′ long terminal repeat; RRE: rev response element; cPPT/CTS: central polypurine tract/central termination sequence; MND: myeloid ...
Sylvie Shen (371528) +4 more
core +1 more source
BMC Biotechnology, 2009 Background Lentiviral vectors are well suited for gene therapy because they can mediate long-term expression in both dividing and nondividing cells. However, lentiviral vectors seem less suitable for liver gene therapy because systemically administered ...
Hiralall Johan K +4 more
doaj +1 more source
Protection of stem cell-derived lymphocytes in a primate AIDS gene therapy model after in vivo selection [PDF]
, 2009 Background: There is currently no effective AIDS vaccine, emphasizing the importance of developing alternative therapies. Recently, a patient was successfully transplanted with allogeneic, naturally resistant CCR5-negative (CCR5 delta 32) cells, setting ...
Trobridge, Grant D. +28 more
core +1 more source
Effectiveness of gene delivery systems for pluripotent and differentiated cells
Molecular Therapy: Methods & Clinical Development, 2015 Human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC) assert a great future for the cardiovascular diseases, both to study them and to explore therapies. However, a comprehensive assessment of the viral vectors used to modify these
Kleopatra Rapti +6 more
doaj +1 more source
Kinetics of lentiviral vector transduction in human CD34+ cells [PDF]
Experimental Hematology, 2016 Unlike cell lines, human hematopoietic stem cells (HSCs) are less efficiently transduced with HIV-1 vectors, potentially limiting this approach. To investigate which step (internalization, reverse transcription, nuclear transport, and integration) limits lentiviral transduction, we evaluated the kinetics of lentiviral transduction in human CD34 ...
Naoya, Uchida +5 more
openaire +2 more sources
FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Generation of stably expressing IRF5 spliced isoform in Jurkat cells
Progress in Microbes and Molecular Biology, 2020 Lentiviral transduction enables the generation of gain-of-function of a targeted gene in mammalian cells. Single-cell cloning through limiting dilution can establish a population of cells with homogenous transgene expression for exploring protein ...
Ashwinder Kaur, Chee-Mun Fang
doaj +1 more source
Vpx-Independent Lentiviral Transduction and shRNA-Mediated Protein Knock-Down in Monocyte-Derived Dendritic Cells. [PDF]
PLoS ONE, 2015 The function of dendritic cells (DCs) in the immune system is based on their ability to sense and present foreign antigens. Powerful tools to research DC function and to apply in cell-based immunotherapy are either silencing or overexpression of genes ...
Wojciech Witkowski +7 more
doaj +1 more source
Optimizing Lentiviral Vector Transduction of Hematopoietic Stem Cells for Gene Therapy
Gene Therapy, 2020 Autologous gene therapy using lentiviral vectors (LVs) holds promise for treating monogenetic blood diseases. However, clinical applications can be limited by suboptimal hematopoietic stem cell (HSC) transduction and insufficient quantities of available ...
Yoon-Young Jang +11 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau +36 more
wiley +1 more source