Results 81 to 90 of about 368,855 (303)

Efficient Transduction and Expansion of Ovine Macrophages for Gene Therapy Implementations

Veterinary Sciences, 2018
A number of bacteria provoking zoonotic diseases present intracellular survival and a host cell tropism limited to the monocyte/macrophage lineage. Thus, infection is rendered difficult to eradicate, causing chronic inflammatory reactions to the host and
Garyfalia Karponi, Spyridon Kritas, Evanthia Petridou, Eleni Papanikolaou   +3 more
doaj   +1 more source

Improved in-vivo airway gene transfer via magnetic-guidance, with protocol development informed by synchrotron imaging

Scientific Reports, 2022
Gene vectors to treat cystic fibrosis lung disease should be targeted to the conducting airways, as peripheral lung transduction does not offer therapeutic benefit. Viral transduction efficiency is directly related to the vector residence time.
Martin Donnelley, Patricia Cmielewski, Kaye Morgan, Juliette Delhove, Nicole Reyne, Alexandra McCarron, Nathan Rout-Pitt, Victoria Drysdale, Chantelle Carpentieri, Kathryn Spiers, Akihisa Takeuchi, Kentaro Uesugi, Naoto Yagi, David Parsons   +13 more
doaj   +1 more source

Transduction of Cultured Cells with Recombinant Lentiviral Particles [PDF]

, 2020
Abstract This protocol describes the transduction of cultured cells with recombinant lentivirus expressing fluorescent markers. The attached methods document is a formal version of the information included here.Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication ...
Samantha C. Faber, Eva C.M. Vitucci, Shaun D. McCullough   +2 more
openaire   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Rescue of lentiviral vector transduction by artificial LEDGF-hybrids. [PDF]

, 2016
LEDGF-fusions were evaluated for their ability to support lentiviral vector transduction. LEDGF-depleted HelaP4-based cell lines stably complemented with LEDGF-hybrids were challenged with a VSV-G pseudo-typed lentiviral reporter vector encoding enhanced
Lenard S. Vranckx (3278832), Jonas Demeulemeester (180282), Zeger Debyser (20227), Rik Gijsbers (20228)   +3 more
core   +1 more source

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Lentiviral transduction and establishment of transgenic hNPCs. [PDF]

, 2015
(A) Schematic representation of the experimental procedure. (B) Immunofluorescence labeling of undifferentiated (day 0) and differentiated (day 14) hNPCs, following lentiviral transduction.
Jennifer Richardson (125536), Muriel Coulpier (499103), Alexandra Huttin (731618), Aurélie Poulet (731620), Daniel Gonzalez-Dunia (61852), Chloé Scordel (731617), Marielle Cochet-Bernoin (731619), Marc Eloit (157053), Marion Szelechowski (653705), Alexandra Benchoua (46532)   +9 more
core   +1 more source

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

Lentiviral vectors for gene therapy of Gaucher disease [PDF]

, 2015
Gaucher disease (GD), a recessive disorder characterised by hepatosplenomegaly, pancytopenia and skeletal complications, is caused by deficiency of the enzyme glucocerebrosidase (GC).
Aitchison, KL
core  

Kupffer cells and not liver sinusoidal endothelial cells prevent lentiviral transduction of hepatocytes [PDF]

, 2005
Lentiviral vectors can stably transduce dividing and nondividing cells in vivo and are best suited to long-term correction of inherited liver diseases. Intraportal administration of lentiviral vectors expressing green fluorescent protein (Lenti-GFP) in ...
van der Rijt, Roos, Seppen, Jurgen, Kunne, Cindy, van Til, Niek P., Hiralall, Johan K., Frederiks, Wilma M., Oude-Elferink, Ronald P. J., Markusic, David M., Vreeling, Heleen   +8 more
core   +1 more source