Results 101 to 110 of about 368,855 (303)

Prospects for transgenesis in the chick [PDF]

, 2004
Research to develop a useful method for genetic modification of the chick has been on-going since the first demonstrations in the mouse in the 1980s that genetic modification is an invaluable tool for the study of gene function. Manipulation of the chick
Sang, Helen
core   +1 more source

PGE2 and Poloxamer Synperonic F108 Enhance Transduction of Human HSPCs with a β-Globin Lentiviral Vector

Molecular Therapy: Methods & Clinical Development, 2019
Lentiviral vector (LV)-based hematopoietic stem and progenitor cell (HSPC) gene therapy is becoming a promising alternative to allogeneic stem cell transplantation for curing genetic diseases.
Katelyn E. Masiuk, Ruixue Zhang, Kyle Osborne, R. Hollis, Beatriz Campo-Fernández, D. Kohn   +5 more
semanticscholar   +1 more source

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

High Cytotoxic Efficiency of Lentivirally and Alpharetrovirally Engineered CD19-Specific Chimeric Antigen Receptor Natural Killer Cells Against Acute Lymphoblastic Leukemia

Frontiers in Immunology, 2020
Autologous chimeric antigen receptor-modified (CAR) T cells with specificity for CD19 showed potent antitumor efficacy in clinical trials against relapsed and refractory B-cell acute lymphoblastic leukemia (B-ALL). Contrary to T cells, natural killer (NK)
Stephan Müller, Stephan Müller, Tobias Bexte, Tobias Bexte, Tobias Bexte, Veronika Gebel, Veronika Gebel, Franziska Kalensee, Franziska Kalensee, Eva Stolzenberg, Eva Stolzenberg, Jessica Hartmann, Ulrike Koehl, Ulrike Koehl, Ulrike Koehl, Axel Schambach, Axel Schambach, Winfried S. Wels, Winfried S. Wels, Winfried S. Wels, Ute Modlich, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich, Evelyn Ullrich   +24 more
doaj   +1 more source

Affinity‐Tuned Albumin Hitchhiking Extends the Bioorthogonal Capture Window in Pretargeting Radiotheranostics

Advanced Healthcare Materials, EarlyView.
Pharmacokinetic engineering of clickable radioligands was achieved via albumin hitchhiking approach within a pretargeted radiotheranostics delivery platform. This approach reduced rapid renal elimination, expanded bioorthogonal capture window, boosted tumor uptake by ∼3.5‐fold, and improved the tumor‐to‐liver ratio by ∼6‐fold.
Xie He, Nicholas L. Fletcher, Gayathri R. Ediriweera, Yifei Zhu, James Humphries, Christopher B. Howard, Jiazheng Wang, Craig A. Bell, Kristian Kempe, Kristofer J. Thurecht   +9 more
wiley   +1 more source

Influence of recombinant histone H1.3 on the efficiency of lentiviral transduction of human cells in vitro [PDF]

, 2020
Lentiviral vectors are widely used in genetic modification of human and animal cells (lentiviral transduction) to enhance their therapeutic potential by expression of recombinant protective and trophic factors.
Solovyeva V., Genkin D., Isaev A., Rizvanov A.   +3 more
core  

Genomic insertion of lentiviral DNA circles directed by the yeast Flp recombinase

BMC Biotechnology, 2008
Background Circular forms of viral genomic DNA are generated during infection of cells with retroviruses like HIV-1. Such circles are unable to replicate and are eventually lost as a result of cell division, lending support to the prevalent notion that ...
Yáñez-Muñoz Rafael J, Jakobsen Maria, Staunstrup Nicklas H, Moldt Brian, Mikkelsen Jacob G   +4 more
doaj   +1 more source

A Lightweight, 3D‐Printable, Low‐Cost, “One‐Click” Dorsal Skin Window for High‐Quality Long‐Term Multimodal Subcutaneous Tumor Imaging

Advanced Healthcare Materials, EarlyView.
An innovative, lightweight 3D‐printed skinfold chamber system is presented for long‐term intravital imaging. This affordable, biocompatible platform simplifies surgical implantation and allows high‐resolution, multimodal visualization of the tumor microenvironment for up to four weeks.
Iván Cortés Domínguez, Xabier Morales Urteaga, Maider Esparza, Lucía Grande, Ainhoa Urbiola, Cristina Ederra, Tomas Muñoz Santoro, Beatrice Pinci, Alvaro Teijeira, Carlos Ortiz de Solórzano   +9 more
wiley   +1 more source

Development and application of non-integrating lentiviral vectors for gene therapy [PDF]

, 2009
Lentiviruses stably integrate their genome into the host genome. Although this feature can be advantageous for long term transgene expression, it also has the potential to cause mutagenesis and cell transformation.
Apolonia, L.F.S.
core  

Apoptosis mediated by lentiviral TRAIL transfer involves transduction-dependent and -independent effects [PDF]

, 2007
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent, which selectively induces apoptosis in many transformed cells without apparent toxic side effects in normal tissue.
Haas, T. L.
core   +1 more source