Results 111 to 120 of about 368,855 (303)

Vectofusin-1 Improves Transduction of Primary Human Cells with Diverse Retroviral and Lentiviral Pseudotypes, Enabling Robust, Automated Closed-System Manufacturing

Human Gene Therapy, 2019
Cell and gene therapies are finally becoming viable patient treatment options, with both T cell- and hematopoietic stem cell (HSC)-based therapies being approved to market in Europe.
C. Radek, O. Bernadin, Katharina Drechsel, N. Cordes, Rita Pfeifer, Pia Sträßer, M. Mormin, A. Gutierrez-Guerrero, F. Cosset, A. Kaiser, Thomas Schaser, A. Galy, E. Verhoeyen, I. Johnston   +13 more
semanticscholar   +1 more source

Lentiviral vectors: optimization of packaging, transduction and gene expression [PDF]

The Journal of Gene Medicine, 2004
Gene transfer vectors based on retroviruses including oncogenic retroviruses and lentiviruses provide effective means for the delivery, integration and expression of exogenous genes in mammalian cells. Lentiviral (LV) vectors provide attractive gene delivery vehicles in the context of non-dividing cells.
openaire   +2 more sources

Encoded Cell‐Material Interactions to Reroute Cytokine Signaling for Regenerative Medicine

Advanced Healthcare Materials, EarlyView.
We present native MATRIX (Material Activated To Regulate Inducible gene eXpression), a co‐engineered material‐cell platform. Surfaces functionalized with antibodies for endogenous soluble ligands (e.g., IL‐1β or IL‐6) capture and immobilize ligands for presentation to a cognate, engineered synthetic Notch receptor, releasing a transcription factor (TF)
Zachary M. Eidman, Jhanvi Sharma, Joanne C. Lee, Nicholas F. Schulze, Hannah J. Brien, Kevin C. Corn, Marjan Rafat, Jonathan M. Brunger   +7 more
wiley   +1 more source

Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells

Retrovirology, 2004
Background Dendritic cells (DCs) are professional antigen-presenting cells that play important roles during human immunodeficiency virus type 1 (HIV-1) infection.
Chang Lung-Ji, He Jin, Chen Xiaochuan
doaj   +1 more source

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells

Advanced Materials, EarlyView.
This study demonstrates plasmonic enhancement of the function of fluorescent voltage sensing proteins (genetically encoded voltage indicators, (GEVIs), QuasAr6) in live mammalian cells. Coupling to plasmonic nanoparticles does not just increase fluorescence, but influences the protein photocycle, creating a hybrid sensor with its response speed to ...
Marco Locarno, Qiangrui Dong, Marco Post, Xin Meng, Cristiano Glessi, Nynke Marije Hettema, Nidas Brandsma, Sebbe Blokhuizen, Alejandro Castañeda Garcia, Srividya Ganapathy, Thieme Schmidt, Lars van Roemburg, Bing Xu, Chun‐Ting Cho, Liedewij Laan, Miao‐Ping Chien, Daan Brinks   +16 more
wiley   +1 more source

A Scalable Lentiviral Workflow for Laboratory-Scale Generation of BCMA/GPRC5D Co-Transduced CAR-T Cells in Multiple Myeloma

Current Issues in Molecular Biology
Efficient and reproducible lentiviral vector production and T-cell transduction remain important technical challenges in CAR-T (Chimeric Antigen Receptor T-cell) cell manufacturing.
Ewa Nowak, Emilia Morawiec, Adam Pudełko, Agnieszka Polak, Mateusz Broncel, Daria Matczyńska, Dawid Zamojski, Michał Czerwinski, Anna Bednarska-Czerwińska   +8 more
doaj   +1 more source

Evaluation of Epstein-Barr Virus Latent Membrane Protein 2 Specific T-Cell Receptors Driven by T-Cell Specific Promoters Using Lentiviral Vector

Clinical and Developmental Immunology, 2011
Transduction of latent membrane protein 2 (LMP2)-specific T-cell receptors into activated T lymphocytes may provide a universal, MHC-restricted mean to treat EBV-associated tumors in adoptive immunotherapy.
Dongchang Yang, Qing Shao, Hua Sun, Xiaoxin Mu, Yun Gao, Runqiu Jiang, Jiajie Hou, Kun Yao, Yun Chen, Beicheng Sun   +9 more
doaj   +1 more source

Aberrant Clonal Hematopoiesis following Lentiviral Vector Transduction of HSPCs in a Rhesus Macaque.

Molecular Therapy, 2019
Lentiviral vectors (LVs) are used for delivery of genes into hematopoietic stem and progenitor cells (HSPCs) in clinical trials worldwide. LVs, in contrast to retroviral vectors, are not associated with insertion site-associated malignant clonal ...
Diego A. Espinoza, Xing Fan, Didi Yang, Stefan Cordes, Lauren L. Truitt, K. Calvo, Idalia M. Yabe, Selami Demirci, Kristin J. Hope, S. Hong, Allen E. Krouse, M. Metzger, Aylin C. Bonifacino, Rong Lu, N. Uchida, J. Tisdale, Xiaolin Wu, S. DeRavin, H. Malech, R. Donahue, Chuanfeng Wu, C. Dunbar   +21 more
semanticscholar   +1 more source

Optimizing of the lentiviral transduction for CAR-lymphocytes

Immunopathology, Allergology, Infectology
The efficacy of CAR therapy is associated with the number of immune cells expressing CAR. Both viruses and non-viral vectors are used to introduce the CAR sequence into the lymphocyte genome, among which the lentiviral method of gene delivery is preferred.
P Fedorova, I Chikileva, N Persiyantseva, M Zamkova, M Kiselevskiy   +4 more
openaire   +1 more source

Malectin Alleviates Endoplasmic Reticulum Stress in Gestational Diabetes Mellitus via Glycoprotein Quality Control Mechanisms

Advanced Science, EarlyView.
Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu, Yumeng Zhang, Ye Wang, Xiaoyu Zhu, Ailin Yuan, Weijing Yin, Huangmin Yu, Xuemin Pang, Yufeng He, Yuchen Wang, Tong Wang, Yong Li, Yunlong Si   +12 more
wiley   +1 more source