Results 111 to 120 of about 260,635 (281)
Advanced Science, EarlyView.A mechanically activated SynNotch reporter platform enables detection of force transmission through receptors as they mediate immune cell interactions. Targeting CD40 on B cells and TCR on T cells records force‐induced reporter activation across 2D coculture, 3D organoid, and implanted in mice, which also reveal force‐amplification of immune receptor ...
Menglan Li +9 more
wiley +1 more source
Characterization of CAR-T transduction parameters using a lentiviral vector [PDF]
, 2019 Production of chimeric antigen receptor T cells (CAR-T) can be accomplished using a viral vector to deliver the transgene. Lentiviral vectors (LVV) are commonly used for transduction due to their safety profile, stable transduction, ease of use, and ...
Shorr, Ryan +3 more
core +1 more source
Skeletal Muscle, 2017 Background shRNA lentiviral vectors are extensively used for gene knockdowns in mammalian cells, and non-target shRNAs typically are considered the proper experimental control for general changes caused by RNAi.
Yutong Liu, Irina Conboy
doaj +1 more source
, 2011 Neutrophils are terminally differentiated cells with a short life-span due to constitutive apoptosis. Because of these characteristics, genetic manipulation of neutrophils has been difficult, although it is highly desired given the importance of ...
Simon, Hans-Uwe +15 more
core +1 more source
Efficient transduction of cytotoxic and anti-HIV-1 genes by a gene-regulatable lentiviral vector. [PDF]
, 2009 Lentiviral vectors modified from human immunodeficiency virus type 1 (HIV-1) offer a promising approach for gene therapy, facilitating transduction of genes into non-dividing cells both in vitro and in vivo.
Hieda, Kuniko +7 more
core +1 more source
Macrophage PABPC4‐SPP1 Axis Orchestrates Immunosuppression in Colorectal Cancer
Advanced Science, EarlyView.In the CRC microenvironment, macrophage PABPC4 binds to the 3′UTR of SPP1 mRNA to stabilize its expression, thereby sustaining M2‐like immunosuppressive macrophage polarization. Concurrently, this axis suppresses CD8+ T cell effector functions via CD44 signaling, collectively fostering an immunosuppressive niche that drives tumor progression.
Meng Wang +14 more
wiley +1 more source
Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic modifications.
PLoS ONE, 2012 Epigenetic modifications may occur during in vitro manipulations of stem cells but these effects have remained unexplored in the context of cell and gene therapy protocols.
Yoshiaki Yamagata +10 more
doaj +1 more source
Effective CD44 lentiviral transduction of NPCs :
, 2013 (A) FACS analysis and (B) immunoblot of CD44-c-myc transduced cells with an antibody revealing CD44-c-myc and endogenous cm-myc showing c-myc (and thus CD44) overexpression in transduced cells.
Pierre Olivier Couraud (383879) +7 more
core +1 more source
Advanced Science, EarlyView.In this study, we identify a novel functional role of ZBTB18 in regulating trigeminal‐mediated neuropathic pain. Nerve injury reduces ZBTB18 in trigeminal ganglion neurons, impairing CHD4/NuRD recruitment and de‐repressing Clic1. Elevated CLIC1 enhances chloride channel activity and neuronal hyperexcitability, thereby driving pain.
Shoupeng Wang +11 more
wiley +1 more source
Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
Molecular Therapy: Nucleic Acids, 2018 Delivery of genes to mouse liver is routinely accomplished by tail-vein injections of viral vectors or naked plasmid DNA. While viral vectors are typically injected in a low-pressure and -volume fashion, uptake of naked plasmid DNA to hepatocytes is ...
Trine Dalsgaard +10 more
doaj +1 more source