Results 121 to 130 of about 368,855 (303)

Optimized lentiviral transduction of human amniotic mesenchymal stromal cells [PDF]

, 2018
Mesenchymal stromal cells are excellent candidates for regenerative medicine since they are multipotent, easy to isolate, can be expanded to obtain clinically relevant numbers and are immunoprivileged.
Ciuffreda, Maria Chiara, Maria Chiara Ciuffreda, Pisano, Federica, Federica Calabrò, Massimiliano Gnecchi, Calabrò, Federica, Gnecchi, Massimiliano, Manuela Mura, Nicola Lanzo, Mura, Manuela, Lanzo, Nicola, Federica Pisano   +11 more
core   +1 more source

A Distinct Subset of Highly Proliferative and Lentiviral Vector (LV)-Transducible NK Cells Define a Readily Engineered Subset for Adoptive Cellular Therapy

Frontiers in Immunology, 2019
Genetic engineering is an important tool for redirecting the function of various types of immune cells and their use for therapeutic purpose. Although NK cells have many beneficial therapeutic features, genetic engineering of immune cells for targeted ...
Rafijul Bari, Markus Granzin, Kam Sze Tsang, Andre Roy, Winfried Krueger, Rimas Orentas, Dina Schneider, Rita Pfeifer, Nina Moeker, Els Verhoeyen, Els Verhoeyen, Boro Dropulic, Wing Leung   +12 more
doaj   +1 more source

Prostaglandin E2 Increases Lentiviral Vector Transduction Efficiency of Adult Human Hematopoietic Stem and Progenitor Cells

Molecular Therapy, 2017
Gene therapy currently in development for hemoglobinopathies utilizes ex vivo lentiviral transduction of CD34+ hematopoietic stem and progenitor cells (HSPCs).
Garrett C. Heffner, M. Bonner, Lauryn M Christiansen, F. J. Pierciey, D. Campbell, Y. Smurnyy, Wenlian Zhang, A. Hamel, S. Shaw, Gretchen Lewis, Kendrick A. Goss, Olivia Garijo, B. Torbett, H. Horton, M. Finer, P. Gregory, G. Veres   +16 more
semanticscholar   +1 more source

Inhibition of IGFBP4 in Granulosa Cells Improves Reproductive Performance and Maintains Fertility With Age via YAP Signaling

Advanced Science, EarlyView.
IGFBP4 is upregulated in granulosa cells of aged ovaries across monkeys, mice, and humans. It inhibits YAP signaling, thereby suppressing cell proliferation and contributing to follicular dysfunction. Deletion of Igfbp4 in granulosa cells enhances ovulatory output, improves hormone profiles, and reproductive performance in aged female mice, suggesting ...
Qianhui Hu, Ajun Geng, Ziyuan Li, Fanghao Guo, Jingqiang Wang, Xinyi Chen, Meiling Zhang, Yi Arial Zeng, Wen Li   +8 more
wiley   +1 more source

Protein overexpression following lentiviral infection of primary mature neutrophils is due to pseudotransduction [PDF]

, 2011
Neutrophils are terminally differentiated cells with a short life-span due to constitutive apoptosis. Because of these characteristics, genetic manipulation of neutrophils has been difficult, although it is highly desired given the importance of ...
Simon, Hans-Uwe, Hans-Uwe Simon, Federzoni, Elena, Schmidt-Mende, Jan, Barbara Geering, Elena Federzoni, Stoeckle, Christina, Jan Schmidt-Mende, Christina Stoeckle, Geering Truffer, Barbara   +9 more
core   +1 more source

The virion-associated incoming HIV-1 RNA genome is not targeted by RNA interference

Retrovirology, 2006
Background RNA interference (RNAi) has proven to be a powerful tool to suppress gene expression and can be used as a therapeutic strategy against human pathogenic viruses such as human immunodeficiency virus type 1 (HIV-1).
Berkhout Ben, ter Brake Olivier, Westerhout Ellen M   +2 more
doaj   +1 more source

Generation of a caged lentiviral vector through an unnatural amino acid for photo-switchable transduction

Nucleic Acids Research, 2019
Application of viral vectors in gene delivery is attracting widespread attention but is hampered by the absence of control over transduction, which may lead to non-selective transduction with adverse side effects.
Yan Wang, Shuai Li, Zhenyu Tian, Jiaqi Sun, Shuobin Liang, Bo Zhang, Lu Bai, Yuanjie Zhang, Xueying Zhou, Sulong Xiao, Qiang Zhang, Lihe Zhang, Chuanling Zhang, Demin Zhou   +13 more
semanticscholar   +1 more source

Perivascular Matrix Densification Dysregulates Angiogenesis and Activates Pro‐Inflammatory Endothelial Cells

Advanced Science, EarlyView.
Perivascular matrix densification promotes the emergence of aberrant endothelial tip cells (ATECs) that invade and persist within fibrotic microenvironments. Using in vivo lineage tracing and a human microvessel model, this study shows that fibrous matrix cues destabilize VE‐cadherin–mediated junctions to gate TGF‐β signaling, inducing a pro ...
Jingyi Xia, William Y. Wang, Kyle A. Jacobs, Kairav Maniar, Daphne Lin, Evan H. Jarman, Daniel L. Matera, Kristen Loesel, Christopher D. Davidson, Harrison L. Hiraki, Xiaotian Tan, Eve H. Shikanov, Robert N. Kent, Carole Parent, Xudong Fan, Ariella Shikanov, Matthew L. Kutys, Brendon M. Baker   +17 more
wiley   +1 more source

Production of Lentiviral Vector Expressing MicroRNA-148b

مجله دانشکده پزشکی اصفهان, 2017
Background: Micro (mi)RNAs are non-coding endogenous RNAs which regulate gene expression by hybridization to specific binding sites in target mRNA sequences.
Samaneh Mollazadeh, Vajiheh Neshati, Bibi Sedigheh Fazly Bazzaz, Majid Mojarrad, Mohammad Amin Kerachian   +4 more
doaj  

Targeting UXS1‐Dependent Glucuronate Detoxification Potentiates Metformin's Anti‐Tumor Efficacy in Lung Adenocarcinoma

Advanced Science, EarlyView.
This study reveals that metformin promotes glucuronic acid metabolism in lung adenocarcinoma by activating UGDH S476 phosphorylation and enhancing the conversion of UDPG to UDPGA based on metabolomics analysis. Through compound virtual screening, it is found that plantainoside targeting UGDH downstream UXS1 leads to UDPGA toxicity accumulation ...
Qihai Sui, Zhencong Chen, Guangyao Shan, Zhengyang Hu, Xing Jin, Jiaqi Liang, Yanjun Yi, Jiacheng Yin, Haochun Shi, Xifei Jiang, Junjie Xi, Zongwu Lin, Cheng Zhan, Fenghao Sun, Wei Jiang   +14 more
wiley   +1 more source