Results 131 to 140 of about 368,855 (303)

Selective transduction of astrocytic and neuronal CNS subpopulations by lentiviral vectors pseudotyped with Chikungunya virus envelope [PDF]

, 2017
Lentiviral vectors are gene delivery vehicles that integrate into the host genome of dividing and non-dividing mammalian cells facilitating long-term transgene expression.
Mazarakis, ND, Sgourou, A, Simmons, LE, Sanchez -Garrido, J, Gao, Y, Eleftheriadou, I, Poh, XY, Dieringer, M   +7 more
core   +1 more source

LC3B Mediated SETDB1‐Accounted Alcoholic Steatohepatitis via Lipidation‐Dependent LAP and Lipidation‐Independent Nuclear Stabilization

Advanced Science, EarlyView.
SETDB1 is progressively downregulated in ALD, correlating with disease severity. SETDB1 deficiency impairs LAP by disrupting Rubicon membrane localization, leading to defective lipid droplet clearance. Concurrently, loss of SETDB1 reduces nuclear LC3B, causing R‐loop accumulation and cGAS‐STING‐driven inflammation. Lipidated LC3B mediates LAP‐dependent
Yi Zhang, Tan Wei, Jiahang Wu, Chuixu Lin, Dongbo Zhu, Yanhui Li, Shuting Shi, Shishun Huang, Leiming Jiang, Hongzhi Wang, Meiqi Song, Pengfei Gao, Xu Wu, Mingjian Fan, Chaofeng Wei, Qian Wang, Lihui Qu, Zhigang Wang   +17 more
wiley   +1 more source

Serum-free Erythroid Differentiation for Efficient Genetic Modification and High-Level Adult Hemoglobin Production

Molecular Therapy: Methods & Clinical Development, 2018
In vitro erythroid differentiation from primary human cells is valuable to develop genetic strategies for hemoglobin disorders. However, current erythroid differentiation methods are encumbered by modest transduction rates and high baseline fetal ...
Naoya Uchida, Selami Demirci, Juan J. Haro-Mora, Atsushi Fujita, Lydia N. Raines, Matthew M. Hsieh, John F. Tisdale   +6 more
doaj   +1 more source

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

Analysis of Hepatic Lentiviral Vector Transduction: Implications for Preclinical Studies and Clinical Gene Therapy Protocols

Viruses
Lentiviral vector-transduced T cells were approved by the FDA as gene therapy anti-cancer medications. Little is known about the effects of host genetic variation on the safety and efficacy of the lentiviral vector gene delivery system.
Peirong Hu, Yajing Hao, Wei Tang, Graham H. Diering, Fei Zou, Tal Kafri   +5 more
doaj   +1 more source

Intravital Multimodal Imaging of Human Cortical Organoid Transplantation in a Mouse Model of Chronic Stroke

Advanced Science, EarlyView.
A multimodal intravital imaging platform enables longitudinal tracking of human cortical organoids transplanted into chronic stroke lesions. By combining surgical microscopy, MRI, bioluminescence imaging, and two‐photon fluorescence microscopy, the platform captures graft placement, viability dynamics, and cellular‐scale morphology in vivo, offering a ...
Jinghui Wang, Guanda Qiao, Honglin Tan, Colleen Russel, Baixuan Yang, Mengyang Jacky Li, Kexin Wang, Jiadi Xu, Chengyan Chu, Miroslaw Janowski, Tian‐Ming Fu, Piotr Walczak, Yajie Liang   +12 more
wiley   +1 more source

Development and validation of a droplet digital PCR method for quantifying lentiviral vector infectious titer

Heliyon
Lentiviruses, with their high transduction efficiency and gene expression levels, are widely used as gene delivery vectors in the development of chimeric antigen receptor T cells (CAR-T) and other genetically modified cell therapies.
Xueling Wu, Xiaoya Zhou, Yueming Wang, Jian Wu, Qian Liang, Xu Yang, Kehua Zhang, Shufang Meng   +7 more
doaj   +1 more source

Agnuside Stabilizes the Complex I Assembly Factor NDUFAF6 to Reinforce Mitochondrial Efficiency and Thermogenic Responsiveness

Advanced Science, EarlyView.
ABSTRACT Brown and beige adipocytes dissipate energy as heat, yet effective strategies to enhance their mitochondrial efficiency remain limited. Here, we identify Agnuside (AGN) as a selective stabilizer of the complex I assembly factor NDUFAF6. AGN directly binds cytosolic NDUFAF6, suppresses its ubiquitination, prolongs its half‐life, and facilitates
Qingwen Zhao, Li Xie, Qianzhuo Wang, Shuying Dai, Bei Li, Yingjuan Zhang, Zhe Sun, Yue Gao   +7 more
wiley   +1 more source

Influence of recombinant histone H1.3 on the efficiency of lentiviral transduction of human cells in vitro [PDF]

, 2012
Lentiviral vectors are widely used in genetic modification of human and animal cells (lentiviral transduction) to enhance their therapeutic potential by expression of recombinant protective and trophic factors.
Solovyeva V., Genkin D., Isaev A., Rizvanov A.   +3 more
core  

Unexpected evolutionarily conserved rapid effects of viral infection on oxytocin receptor and TGF-β/pSmad3

Skeletal Muscle, 2017
Background shRNA lentiviral vectors are extensively used for gene knockdowns in mammalian cells, and non-target shRNAs typically are considered the proper experimental control for general changes caused by RNAi.
Yutong Liu, Irina Conboy
doaj   +1 more source