Results 71 to 80 of about 260,635 (281)

Lentiviral transduction v1

Lentiviral transduction of primary neurons
Bishal Basak, Erika Holzbaur
openaire   +1 more source

Remote Activation of Wnt Signaling and Cell Proliferation by E‐Cadherin Magnetomechanical Stimulation

Advanced Functional Materials, EarlyView.
A pioneering magnetogenetic toolkit enables non‐invasive, precise spatiotemporal modulation of intracellular Wnt/β‐catenin signaling. Functionalized magnetic nanoparticles (MNPs@E/EC15) target cellular E‐cadherins to generate localized magnetomechanical forces under low‐intensity magnetic fields.
Christian Castro‐Hinojosa, Pablo Martínez‐Vicente, Lucía G. Recaredo, Susel Del Sol‐Fernández, Pilar Gomollón‐Zueco, Yilian Fernández‐Afonso, Raluca M. Fratila, María Moros   +7 more
wiley   +1 more source

High efficiency closed-system gene transfer using automated spinoculation

Journal of Translational Medicine, 2021
Background Gene transfer is an important tool for cellular therapies. Lentiviral vectors are most effectively transferred into lymphocytes or hematopoietic progenitor cells using spinoculation.
Victoria Ann Remley, Jianjian Jin, Sarmila Sarkar, Larry Moses, Michaela Prochazkova, Yihua Cai, Lipei Shao, Hui Liu, Tatyana Fuksenko, Ping Jin, David F. Stroncek, Steven L. Highfill   +11 more
doaj   +1 more source

Lentiviral vectors for gene therapy of Gaucher disease [PDF]

, 2015
Gaucher disease (GD), a recessive disorder characterised by hepatosplenomegaly, pancytopenia and skeletal complications, is caused by deficiency of the enzyme glucocerebrosidase (GC).
Aitchison, KL
core  

Rescue of lentiviral vector transduction by artificial LEDGF-hybrids.

, 2016
LEDGF-fusions were evaluated for their ability to support lentiviral vector transduction. LEDGF-depleted HelaP4-based cell lines stably complemented with LEDGF-hybrids were challenged with a VSV-G pseudo-typed lentiviral reporter vector encoding enhanced
Lenard S. Vranckx (3278832), Jonas Demeulemeester (180282), Zeger Debyser (20227), Rik Gijsbers (20228)   +3 more
core   +1 more source

Apoptosis mediated by lentiviral TRAIL transfer involves transduction-dependent and -independent effects [PDF]

, 2007
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising anticancer agent, which selectively induces apoptosis in many transformed cells without apparent toxic side effects in normal tissue.
Haas, T. L.
core   +1 more source

A methodological scheme illustrates the culture of CD34⁺ cells, lentiviral transduction conditions, erythroid differentiation protocols, and timeframes for gene expression, morphology, and HPLC studies.

, 2023
A methodological scheme illustrates the culture of CD34+ cells, lentiviral transduction conditions, erythroid differentiation protocols, and timeframes for gene expression, morphology, and HPLC studies.
Sukanya Chumchuen (14761903), Orapan Sripichai (479296), Natee Jearawiriyapaisarn (14761906), Suthat Fucharoen (164423), Chayanon Peerapittayamongkol (246613)   +4 more
core   +1 more source

Dual Role of Tamoxifen in Enhancing STING and CEACAM1 Expression to Prime a Favorable Tumor Microenvironment for Anti‐TIM3 Immunotherapy

Advanced Science, EarlyView.
Anti‐PD‐1/PD‐L1 blockade has revolutionized cancer immunotherapy, but is ineffective against endocrine‐treated (i.e., Tamoxifen), relapsed ER+ breast cancer (BC) patients. This study provides insight into the sub‐optimal response of ER+BCs to anti‐PD‐1/PD‐L1 blockade – highlighting the induction of STING and the CEACAM1/TIM3 axis after chronic ...
Marvin Angelo E Aberin, Saurabh Singh, Soumya Chatterjee, Guang‐Zhi Sui, Yi‐Fu Wang, Ya‐Ting Lu, Yu‐Ling Lee, Kun‐Yuan Lin, Joy Khag, Ta‐Yu Liu, Shao‐Han Chang, Wai‐Mui Cheung, Hsiao‐Chin Hong, Ren‐Jun Hsu, Chen‐Yang Shen, Chia‐Wei Li, Weng‐Lang Yang, Yao‐Ming Chang, Shih‐Yu Chen, Chandan Guha, Shu‐Ping Wang   +20 more
wiley   +1 more source

Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction

Viruses, 2020
Lentiviral vectors are versatile tools for gene delivery purposes. While in the earlier versions of retroviral vectors, transgene expression was controlled by the long terminal repeats (LTRs), the latter generations of vectors, including those derived ...
Audrey Page, Floriane Fusil, François-Loïc Cosset   +2 more
doaj   +1 more source

Lentiviral transduction and establishment of transgenic hNPCs.

, 2015
(A) Schematic representation of the experimental procedure. (B) Immunofluorescence labeling of undifferentiated (day 0) and differentiated (day 14) hNPCs, following lentiviral transduction.
Jennifer Richardson (125536), Muriel Coulpier (499103), Alexandra Huttin (731618), Aurélie Poulet (731620), Daniel Gonzalez-Dunia (61852), Chloé Scordel (731617), Marielle Cochet-Bernoin (731619), Marc Eloit (157053), Marion Szelechowski (653705), Alexandra Benchoua (46532)   +9 more
core   +1 more source