Results 51 to 60 of about 260,635 (281)

Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk [PDF]

, 2012
We have previously shown that injury-induced neointima formation was rescued by adenoviral-Nogo-B gene delivery. Integrase-competent lentiviral vectors (ICLV) are efficient at gene delivery to vascular cells but present a risk of insertional mutagenesis.
Sessa, W.C., Chick, H.E., Kane, N.M., Baker, A.H., Thrasher, A.J., Alba, R., Fronza, R., McDonald, R.A., Delles, C., Schmidt, M., Nowrouzi, A.   +10 more
core   +1 more source

A role for eIF5A in translational control of stress‐inducible proteins in multiple myeloma cell lines

Molecular Oncology, EarlyView.
Loss of eIF5A rewires thapsigargin‐mediated endoplasmic reticulum stress responses in multiple myeloma cells by selective translation of mRNA transcripts, consequently dampening thapsigargin‐induced unfolded protein response (UPR) activation and shifting the balance from induction of apoptosis toward cell survival and proliferation.
Demi Both, Ingrid A. M. Derks, Raphaël Vanleyssem, Marine Leclercq, Najla El Hachem, Pierre Close, Eric Eldering, Marco Vincent Haselager   +7 more
wiley   +1 more source

Selective transduction of astrocytic and neuronal CNS subpopulations by lentiviral vectors pseudotyped with Chikungunya virus envelope [PDF]

, 2017
Lentiviral vectors are gene delivery vehicles that integrate into the host genome of dividing and non-dividing mammalian cells facilitating long-term transgene expression.
Mazarakis, ND, Sgourou, A, Simmons, LE, Sanchez -Garrido, J, Gao, Y, Eleftheriadou, I, Poh, XY, Dieringer, M   +7 more
core   +1 more source

p190A/ARHGAP35 and p190B/ARHGAP5 proteins in endometrial cancer: a novel cancer‐relevant paralog interplay

Molecular Oncology, EarlyView.
This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault, Capucine Heraud, Mélissa Correia de Oliveira, Véronique Neaud, Valérie Valesco, Valérie Prouzet‐Mauleon, Béatrice Turcq, Jean‐William Dupuy, Anne‐Aurélie Raymond, Sabrina Croce, Frédéric Saltel, Valérie Lagrée, Violaine Moreau   +12 more
wiley   +1 more source

Microscale characterisation of a manufacturing route for lentiviral vectors [PDF]

, 2015
Lentiviral vectors used in clinical trials are currently produced by transient transfection of adherent human embryonic kidney (HEK)293(T) cells. However, this approach is not scalable and for commercialisation the development of alternative strategies ...
Guy, HM
core  

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival

Molecular Oncology, EarlyView.
CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna, Jodie Hay, Michael W. Moles, Lucy Clarke, Jennifer Cassels, Alison Sefcick, Alison M. McCaig, Heather G. Jørgensen, Alison M. Michie   +8 more
wiley   +1 more source

Efficient Transduction and Expansion of Ovine Macrophages for Gene Therapy Implementations

Veterinary Sciences, 2018
A number of bacteria provoking zoonotic diseases present intracellular survival and a host cell tropism limited to the monocyte/macrophage lineage. Thus, infection is rendered difficult to eradicate, causing chronic inflammatory reactions to the host and
Garyfalia Karponi, Spyridon Kritas, Evanthia Petridou, Eleni Papanikolaou   +3 more
doaj   +1 more source

Improved in-vivo airway gene transfer via magnetic-guidance, with protocol development informed by synchrotron imaging

Scientific Reports, 2022
Gene vectors to treat cystic fibrosis lung disease should be targeted to the conducting airways, as peripheral lung transduction does not offer therapeutic benefit. Viral transduction efficiency is directly related to the vector residence time.
Martin Donnelley, Patricia Cmielewski, Kaye Morgan, Juliette Delhove, Nicole Reyne, Alexandra McCarron, Nathan Rout-Pitt, Victoria Drysdale, Chantelle Carpentieri, Kathryn Spiers, Akihisa Takeuchi, Kentaro Uesugi, Naoto Yagi, David Parsons   +13 more
doaj   +1 more source

Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction [PDF]

, 2011
This chapter provides a method for reprogramming human dermal fibroblasts into induced pluripotent stem cells (iPSCs) using three lentiviruses containing cDNAs for OCT4 and SOX2, KLF4 and C-MYC, and NANOG and LIN28, respectively. Lentiviral vectors are based on the human immunodeficiency virus (HIV) and provide an effective means for the delivery ...
Hubert E, Nethercott, David J, Brick, Philip H, Schwartz   +2 more
openaire   +2 more sources