Results 11 to 20 of about 7,843,232 (348)
Multiplex genomic recording of enhancer and signal transduction activity in mammalian cells
bioRxiv, 2021 Measurements of gene expression and signal transduction activity are conventionally performed with methods that require either the destruction or live imaging of a biological sample within the timeframe of interest.
Wei Chen +8 more
semanticscholar +1 more source
Dasatinib is a potent enhancer for CAR T cell generation by CD3-targeted lentiviral vectors
Molecular Therapy: Methods & Clinical Development, 2023 CD3-targeted lentiviral vectors (CD3-LVs) mediate selective transduction of human T lymphocytes in vitro and in vivo while simultaneously activating the targeted cells.
Angela H. Braun +3 more
doaj +1 more source
Frontiers in Immunology, 2023 Chimeric antigen receptor (CAR)-T cell therapy is a groundbreaking immunotherapy for cancer. However, the intricate and costly manufacturing process remains a hurdle.
Lena Rauch-Wirth +13 more
doaj +1 more source
Advanced Science, 2023 Lentiviral vectors (LV) have become the dominant tool for stable gene transfer into lymphocytes including chimeric antigen receptor (CAR) gene delivery to T cells, a major breakthrough in cancer therapy.
Filippos T Charitidis +10 more
doaj +1 more source
Identification of a small molecule for enhancing lentiviral transduction of T cells
Molecular Therapy: Methods & Clinical Development, 2023 Genetic modification of cells using viral vectors has shown huge therapeutic benefit in multiple diseases. However, inefficient transduction contributes to the high cost of these therapies.
Paulina Malach +10 more
doaj +1 more source
Scientific Reports, 2022 Transduction of endothelial cells (EC) with a vector that expresses apolipoprotein A-I (APOAI) reduces atherosclerosis in arteries of fat-fed rabbits. However, the effects on atherosclerosis are partial and might be enhanced if APOAI expression could be ...
Meena Sethuraman +6 more
doaj +1 more source
Gene Therapy, 2020 Adeno-associated viral vectors are widely used as vehicles for gene transfer to the nervous system. The promoter and viral vector serotype are two key factors that determine the expression dynamics of the transgene.
Bart Nieuwenhuis +6 more
semanticscholar +1 more source
The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells. [PDF]
, 2015 Lentiviral vectors designed for the treatment of the hemoglobinopathies require the inclusion of regulatory and strong enhancer elements to achieve sufficient expression of the β-globin transgene.
Baldwin, Kismet M +12 more
core +8 more sources
Co-transduction of dual-adeno-associated virus vectors in the neonatal and adult mouse utricles
Frontiers in Molecular Neuroscience, 2022 Adeno-associated virus (AAV)-mediated gene transfer is an efficient method of gene over-expression in the vestibular end organs. However, AAV has limited usefulness for delivering a large gene, or multiple genes, due to its small packaging capacity (<
Zhong-Rui Chen +19 more
doaj +1 more source
In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. [PDF]
PLoS ONE, 2016 The murine Cebpa gene contains a +37 kb, evolutionarily conserved 440 bp enhancer that directs high-level expression to myeloid progenitors in transgenic mice.
Hong Guo, Stacy Cooper, Alan D Friedman
doaj +1 more source