Results 11 to 20 of about 145,799 (355)

Homeostatic enhancement of sensory transduction [PDF]

Proceedings of the National Academy of Sciences, 2017
Significance How do biological systems ensure robustness of function despite developmental and environmental variation? Although the operation of some systems appears to require precise control over parameter values, we describe how the function of the ear might instead be made robust to parameter perturbation.
Andrew R, Milewski, Dáibhid, Ó Maoiléidigh, Joshua D, Salvi, A J, Hudspeth   +3 more
openaire   +2 more sources

Enhancement of adenoviral transduction with polycationic liposomes in vivo [PDF]

Cancer Gene Therapy, 2000
Although the high transfection efficiency with adenovirus in vitro is well documented, it is still not clear whether adenoviral vectors are effective in vivo in solid tumor models. In our preliminary experiment, transduction of tumor tissue was limited to just around the injection site after intratumoral injection of the adenoviral vector.
S G, Lee, S J, Yoon, C D, Kim, K, Kim, D S, Lim, Y I, Yeom, M W, Sung, D S, Heo, N K, Kim   +8 more
openaire   +2 more sources

Optimized peptide nanofibrils as efficient transduction enhancers for in vitro and ex vivo gene transfer

Frontiers in Immunology, 2023
Chimeric antigen receptor (CAR)-T cell therapy is a groundbreaking immunotherapy for cancer. However, the intricate and costly manufacturing process remains a hurdle.
Lena Rauch-Wirth, Alexander Renner, Kübra Kaygisiz, Tatjana Weil, Laura Zimmermann, Armando A. Rodriguez-Alfonso, Armando A. Rodriguez-Alfonso, Desiree Schütz, Sebastian Wiese, Ludger Ständker, Tanja Weil, Dominik Schmiedel, Jan Münch, Jan Münch   +13 more
doaj   +1 more source

Approaches to enhancing the retroviral transduction of human synoviocytes [PDF]

Arthritis Research & Therapy, 2001
This report concerns a clinical trial for rheumatoid arthritis (RA), approved by the US National Institutes of Health and the Food and Drug Administration. An amphotropic retrovirus (MFG-IRAP) was used ex vivo to transfer a cDNA encoding human interleukin-1 receptor antagonist (IL-1Ra) to synovium.
M A, Del Vecchio, H I, Georgescu, J E, McCormack, P D, Robbins, C H, Evans   +4 more
openaire   +2 more sources

Novel expression cassettes for increasing apolipoprotein AI transgene expression in vascular endothelial cells

Scientific Reports, 2022
Transduction of endothelial cells (EC) with a vector that expresses apolipoprotein A-I (APOAI) reduces atherosclerosis in arteries of fat-fed rabbits. However, the effects on atherosclerosis are partial and might be enhanced if APOAI expression could be ...
Meena Sethuraman, Nagadhara Dronadula, Lianxiang Bi, Bradley K. Wacker, Ethan Knight, Pieter De Bleser, David A. Dichek   +6 more
doaj   +1 more source

The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells. [PDF]

, 2015
Lentiviral vectors designed for the treatment of the hemoglobinopathies require the inclusion of regulatory and strong enhancer elements to achieve sufficient expression of the β-globin transgene.
Baldwin, Kismet M, Campo-Fernandez, Beatriz, Cooper, Aaron R, Hoban, Megan D, Hollis, Roger P, Kaufman, Michael L, Kohn, Donald B, Lumaquin, Dianne, Romero, Zulema, Senadheera, Shantha, Urbinati, Fabrizia, Wang, Xiaoyan, Wherley, Jennifer   +12 more
core   +8 more sources

Fusogenic effects of murine retroviruses and cationic enhancers of transduction [PDF]

Cancer Gene Therapy, 2000
The maturation of retrovirus particles involves proteolytic cleavage of the envelope glycoprotein transmembrane component, resulting in conversion of the virus particle to a fusogenic or infectious state. Susceptible murine cells exposed to virus-containing supernatants from ecotropic retroviral helper cells occasionally fused to neighboring cells ...
G, Xu, F, Solaiman, M A, Zink, C P, Hodgson   +3 more
openaire   +2 more sources

Co-transduction of dual-adeno-associated virus vectors in the neonatal and adult mouse utricles

Frontiers in Molecular Neuroscience, 2022
Adeno-associated virus (AAV)-mediated gene transfer is an efficient method of gene over-expression in the vestibular end organs. However, AAV has limited usefulness for delivering a large gene, or multiple genes, due to its small packaging capacity (<
Zhong-Rui Chen, Zhong-Rui Chen, Jing-Ying Guo, Jing-Ying Guo, Lu He, Lu He, Shan Liu, Shan Liu, Jun-Yi Xu, Jun-Yi Xu, Zi-Jing Yang, Zi-Jing Yang, Wei Su, Wei Su, Ke Liu, Ke Liu, Shu-Sheng Gong, Shu-Sheng Gong, Guo-Peng Wang, Guo-Peng Wang   +19 more
doaj   +1 more source

In Vivo Deletion of the Cebpa +37 kb Enhancer Markedly Reduces Cebpa mRNA in Myeloid Progenitors but Not in Non-Hematopoietic Tissues to Impair Granulopoiesis. [PDF]

PLoS ONE, 2016
The murine Cebpa gene contains a +37 kb, evolutionarily conserved 440 bp enhancer that directs high-level expression to myeloid progenitors in transgenic mice.
Hong Guo, Stacy Cooper, Alan D Friedman
doaj   +1 more source

T cell transduction and suicide with an enhanced mutant thymidine kinase [PDF]

Gene Therapy, 2002
Retroviral transfer of Herpes simplex virus thymidine kinase to T cells has been used to confer sensitivity to the antiviral agent ganciclovir. This has allowed therapeutic approaches to be developed in which T cells mediating graft-versus-host disease after bone marrow transplantation can be selectively eliminated by the administration of ganciclovir.
W, Qasim, A J, Thrasher, J, Buddle, C, Kinnon, M E, Black, H B, Gaspar   +5 more
openaire   +2 more sources