Results 1 to 10 of about 289,359 (292)

Filament flexibility enhances power transduction of F-actin bundles [PDF]

The Journal of Chemical Physics, 2019
The dynamic behavior of bundles of actin filaments growing against a loaded obstacle is investigated through a generalized version of the standard multifilament Brownian Ratchet model in which the (de)polymerizing filaments are treated not as rigid rods but as semiflexible discrete wormlike chains with a realistic value of the persistence length.
Alessia Perilli, Carlo Pierleoni, Jean-Paul Ryckaert   +2 more
openaire   +5 more sources

Targeting Adeno-Associated Virus Vectors for Local Delivery to Fractures and Systemic Delivery to the Skeleton

Molecular Therapy: Methods & Clinical Development, 2019
A panel of 18 recombinant adeno-associated virus (rAAV) variants, both natural and engineered, constitutively expressing Cre recombinase under the cytomegalovirus early enhancer/chicken β actin (CAG) promoter, were screened for their ability to transduce
Lucinda R. Lee, Lauren Peacock, Leszek Lisowski, David G. Little, Craig F. Munns, Aaron Schindeler   +5 more
doaj   +1 more source

High-throughput screening identifies compounds that enhance lentiviral transduction [PDF]

Gene Therapy, 2014
A difficulty in the field of gene therapy is the need to increase the susceptibility of hematopoietic stem cells (HSCs) to ex vivo genetic manipulation. To overcome this obstacle a high-throughput screen was performed to identify compounds that could enhance the transduction of target cells by lentiviral vectors.
J M, Johnston, G, Denning, R, Moot, D, Whitehead, J, Shields, J M, Le Doux, C B, Doering, H T, Spencer   +7 more
openaire   +2 more sources

T cell transduction and suicide with an enhanced mutant thymidine kinase [PDF]

Gene Therapy, 2002
Retroviral transfer of Herpes simplex virus thymidine kinase to T cells has been used to confer sensitivity to the antiviral agent ganciclovir. This has allowed therapeutic approaches to be developed in which T cells mediating graft-versus-host disease after bone marrow transplantation can be selectively eliminated by the administration of ganciclovir.
W, Qasim, A J, Thrasher, J, Buddle, C, Kinnon, M E, Black, H B, Gaspar   +5 more
openaire   +2 more sources

Analysis of the Effect of Methylation Alterations on Enhancer Activity in Cancer

Guoji laonian yixue zazhi
Objective To analyze and identify the impact of methylation changes on enhancer activity in cancer. Methods Obtain enhancers, transcription factor binding sites, and molecular pathway data from the human enhancer database FANTOM5, methylated DNA binding ...
Yunxiao Xie, Na Ding
doaj   +1 more source

Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons

Stem Cells International, 2019
Recombinant adeno-associated virus (rAAV), produced from a nonpathogenic parvovirus, has become an increasing popular vector for gene therapy applications in human clinical trials. However, transduction and transgene expression of rAAVs can differ across
Thu T. Duong, James Lim, Vidyullatha Vasireddy, Tyler Papp, Hung Nguyen, Lanfranco Leo, Jieyan Pan, Shangzhen Zhou, H. Isaac Chen, Jean Bennett, Jason A. Mills   +10 more
doaj   +1 more source

Enhancing the potency of in vivo lentiviral vector mediated gene therapy to hepatocytes

Nature Communications
In vivo gene therapy to the liver using lentiviral vectors (LV) may represent a one-and-done therapeutic approach for monogenic diseases. Increasing LV gene therapy potency is crucial for reducing the effective doses, thus alleviating dose-dependent ...
Cesare Canepari, Michela Milani, Chiara Simoni, Francesco Starinieri, Monica Volpin, Anna Fabiano, Mauro Biffi, Fabio Russo, Rossana Norata, Martina Rocchi, Chiara Brombin, Federica Cugnata, Eugenio Montini, Francesca Sanvito, Markus Grompe, Alessio Cantore   +15 more
doaj   +1 more source

An in vivo systemic massively parallel platform for deciphering animal tissue-specific regulatory function

Frontiers in Genetics
Introduction: Transcriptional regulation is an important process wherein non-protein coding enhancer sequences play a key role in determining cell type identity and phenotypic diversity.
Ashley R. Brown, Ashley R. Brown, Grant A. Fox, Grant A. Fox, Irene M. Kaplow, Irene M. Kaplow, Irene M. Kaplow, Alyssa J. Lawler, Alyssa J. Lawler, BaDoi N. Phan, BaDoi N. Phan, BaDoi N. Phan, Lahari Gadey, Lahari Gadey, Morgan E. Wirthlin, Morgan E. Wirthlin, Easwaran Ramamurthy, Easwaran Ramamurthy, Gemma E. May, Ziheng Chen, Qiao Su, Qiao Su, C. Joel McManus, Robert van de Weerd, Robert van de Weerd, Andreas R. Pfenning, Andreas R. Pfenning, Andreas R. Pfenning   +27 more
doaj   +1 more source

Near-field optomechanical transduction enhanced by Raman gain

Optics Express
Raman-gain-enhanced near-field optomechanical transduction between a movable optical cavity and SiN-membrane resonator is demonstrated. The Raman gain compensates for the intrinsic loss of the cavity and amplifies the optomechanical transduction, through which the membrane vibration is sensed using a high-Q whispering-gallery-mode optical cavity ...
Ryoko Sakuma, Motoki Asano, Hiroshi Yamaguchi, Hajime Okamoto   +3 more
openaire   +3 more sources

Lentiviral vectors for precise expression to treat X-linked lymphoproliferative disease

Molecular Therapy: Methods & Clinical Development
X-linked lymphoproliferative disease (XLP1) results from SH2D1A gene mutations affecting the SLAM-associated protein (SAP). A regulated lentiviral vector (LV), XLP-SMART LV, designed to express SAP at therapeutic levels in T, NK, and NKT cells, is ...
Paul G. Ayoub, Julia Gensheimer, Lindsay Lathrop, Colin Juett, Jason Quintos, Kevin Tam, Jack Reid, Feiyang Ma, Curtis Tam, Grace E. McAuley, Devin Brown, Xiaomeng Wu, Ruixue Zhang, Kathryn Bradford, Roger P. Hollis, Gay M. Crooks, Donald B. Kohn   +16 more
doaj   +1 more source