Results 21 to 30 of about 304,117 (294)

Multiple sequence-specific transcription factors modulate cytomegalovirus enhancer activity in vitro [PDF]

, 1988
The possibility of DNA-binding proteins interacting in vitro with the polymerase II transcriptional machinery was explored by using a competition assay with individual target sequences for enhancer-binding factors.
Lubon, H, Lubon, Henryk, Hennighausen, L, Hennighausen, Lothar, Ghazal, Peter, Ghazal, P   +5 more
core   +1 more source

Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences

Molecular Therapy: Methods & Clinical Development, 2020
Hematopoietic stem cell gene therapy is a promising approach for treating disorders of the hematopoietic system. Identifying combinations of cis-regulatory elements that do not impede packaging or transduction efficiency when included in lentiviral ...
Richard A. Morgan, Feiyang Ma, Mildred J. Unti, Devin Brown, Paul George Ayoub, Curtis Tam, Lindsay Lathrop, Bamidele Aleshe, Ryo Kurita, Yukio Nakamura, Shantha Senadheera, Ryan L. Wong, Roger P. Hollis, Matteo Pellegrini, Donald B. Kohn   +14 more
doaj   +1 more source

STCDB: Signal Transduction Classification Database [PDF]

, 2003
Chen M, Lin S, Hofestädt R. STCDB: Signal Transduction Classification Database. Nucleic Acids Research. 2004;32(90001):D456-D458.The Signal Transduction Classification Database (STCDB) is a database of information relative to the classification of signal
Chen, Ming, Susana Lin, Hofestädt, Ralf, Ralf Hofestaedt, Lin, Susana, Ming Chen   +5 more
core   +1 more source

Tetrameric architecture of an active phenol-bound form of the AAA+ transcriptional regulator DmpR

Nature Communications, 2020
DmpR is a bacterial enhancer binding protein from the AAA+ family of ATPases that binds aromatic compounds and controls the transcription of genes involved in the degradation of toxic pollutants.
Kwang-Hyun Park, Sungchul Kim, Su-Jin Lee, Jee-Eun Cho, Vinod Vikas Patil, Arti Baban Dumbrepatil, Hyung-Nam Song, Woo-Chan Ahn, Chirlmin Joo, Seung-Goo Lee, Victoria Shingler, Eui-Jeon Woo   +11 more
doaj   +1 more source

The strong enhancer element in the immediate early region of the human cytomegalovirus genome [PDF]

, 1985
The human cytomegalovirus (HCMV), a member of the herpesvirus group, was found to possess a strong transcription enhancer in the immediate early gene region. Co-transfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-
Boshart, Michael, Schaffner, Walter, Weber, Frank, Dorsch-Häsler, Karoline, Jahn, Gerhard, Rüger, Rüdiger, Fleckenstein, Bernhard, Stoerker, Jay, Calendar, R., Gold, L.   +9 more
core   +1 more source

Efficient lentiviral transduction method to gene modify cord blood CD8+ T cells for cancer therapy applications

Molecular Therapy: Methods & Clinical Development, 2021
Adoptive T cell therapy utilizing tumor-specific autologous T cells has shown promising results for cancer treatment. However, the limited numbers of autologous tumor-associated antigen (TAA)-specific T cells and the functional aberrancies, due to ...
Vania Lo Presti, Annelisa M. Cornel, Maud Plantinga, Ester Dünnebach, Jurgen Kuball, Jaap Jan Boelens, Stefan Nierkens, Niek P. van Til   +7 more
doaj   +1 more source

Fusogenic effects of murine retroviruses and cationic enhancers of transduction [PDF]

Cancer Gene Therapy, 2000
The maturation of retrovirus particles involves proteolytic cleavage of the envelope glycoprotein transmembrane component, resulting in conversion of the virus particle to a fusogenic or infectious state. Susceptible murine cells exposed to virus-containing supernatants from ecotropic retroviral helper cells occasionally fused to neighboring cells ...
G, Xu, F, Solaiman, M A, Zink, C P, Hodgson   +3 more
openaire   +2 more sources

The Optimized γ-Globin Lentiviral Vector GGHI-mB-3D Leads to Nearly Therapeutic HbF Levels In Vitro in CD34⁺ Cells from Sickle Cell Disease Patients

Viruses, 2022
We have previously demonstrated that both the original γ-globin lentiviral vector (LV) GGHI and the optimized GGHI-mB-3D LV, carrying the novel regulatory elements of the 3D HPFH-1 enhancer and the 3’ β-globin UTR, can significantly increase HbF ...
Ekati Drakopoulou, Maria Georgomanoli, Carsten W. Lederer, Fottes Panetsos, Marina Kleanthous, Ersi Voskaridou, Dimitrios Valakos, Eleni Papanikolaou, Nicholas P. Anagnou   +8 more
doaj   +1 more source

GradinaruLab/in-vitro-transduction-assay: Science Advances Publication

, 2023
This code contains notebooks and files to analyze in vitro images for transduction and ...
David Goertsen
core   +1 more source

Elucidating Enhancer Function of Human Chromosome 19 Fragments in AAV Vectors for Hemophilia Treatment [PDF]

, 2021
Adeno-associated viruses (AAV) are a common gene therapy tool used to deliver custom transgenes to specific targets. Utilizing AAV, researchers engineer non-pathogenic viral vectors to supplement insufficient gene products.
Workley, Luke
core   +1 more source