Results 41 to 50 of about 851,785 (340)

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

Nucleofection as an Efficient Method for Alpha TC1-6 Cell Line Transfection

Applied Sciences, 2022
An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there is a need for an optimized transfection process for individual model systems separately.
Marija Đorđević, Verica Paunović, Maja Jovanović Tucović, Anja Tolić, Jovana Rajić, Svetlana Dinić, Aleksandra Uskoković, Nevena Grdović, Mirjana Mihailović, Ivanka Marković, Jelena Arambašić Jovanović, Melita Vidaković   +11 more
doaj   +1 more source

Headgroup modification of cholesterol-based cationic lipids: Synthesis, transfection efficiency evaluation, and serum compatibility [PDF]

Songklanakarin Journal of Science and Technology (SJST), 2020
There were two objectives for this study. First, it focused on eight lipids having cholesterol as hydrophobic tails, carbamate linkers, and different polar headgroups.
Chopaka Thongbamrer, Nattisa Niyomtham, Chayutra Chaiwut, Charoen Posa, Nuttapon Apiratikul, Ek Sangvichien, Praneet Opanasopit, Uthai Sakee, Boon-ek Yingyongnarongkul, Widchaya Radchatawedchakoon   +9 more
doaj   +1 more source

Inhibiting stearoyl‐CoA desaturase suppresses bone metastatic prostate cancer by modulating cellular stress, mTOR signaling, and DNA damage response

FEBS Letters, EarlyView.
Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson, Mackenzie K Herroon, Shane Mecca, Laimar C Garmo, Jacob Lindquist, Shrila Rajendran, Steve M. Patrick, Izabela Podgorski   +7 more
wiley   +1 more source

High-Throughput Microfluidic Electroporation (HTME): A Scalable, 384-Well Platform for Multiplexed Cell Engineering

Bioengineering
Electroporation-mediated gene delivery is a cornerstone of synthetic biology, offering several advantages over other methods: higher efficiencies, broader applicability, and simpler sample preparation. Yet, electroporation protocols are often challenging
William R. Gaillard, Jess Sustarich, Yuerong Li, David N. Carruthers, Kshitiz Gupta, Yan Liang, Rita Kuo, Stephen Tan, Sam Yoder, Paul D. Adams, Hector Garcia Martin, Nathan J. Hillson, Anup K. Singh   +12 more
doaj   +1 more source

The role of miR-24 as a race related genetic factor in prostate cancer. [PDF]

, 2017
The incidence of prostate cancer (PCa) among African-Americans (AfA) is significantly higher than Caucasian-Americans (CaA) but the genetic basis for this disparity is not known.
Dahiya, Rajvir, Dasgupta, Pritha, Deng, Guoren, Hashimoto, Yutaka, Kato, Taku, Kulkarni, Priyanka, Kumar, Deepak, Majid, Shahana, Mitsui, Yozo, Saini, Sharanjot, Shahryari, Varahram, Shiina, Marisa, Sumida, Mitsuho, Tabatabai, Laura, Tanaka, Yuichiro, Yamamura, Soichiro   +15 more
core   +1 more source

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Gene Therapy with Cytosine Deaminase and Endostatin Fusion Gene Mediated by Endothelial Progenitor Cells in Hepatomas

Cancer Management and Research, 2020
Yue-Lin Zhang,1,2,* Tan-Yang Zhou,1,2,* Jing Ai,3,* Sheng-Qun Chen,1,2,* Feng Chen,4 Chun-Hui Nie,1,2 Xin-Hua Chen,1,2 Guan-Hui Zhou,1,2 Hong-Liang Wang,1,2 Tong-Yin Zhu,1,2 Bao-Quan Wang,1,2 Zi-Niu Yu,1,2 Li Jing,1,2 Li-Ming Wu,1,2 Shu-Sen Zheng,1,2 Jun-
Zhang YL, Zhou TY, Ai J, Chen SQ, Chen F, Nie CH, Chen XH, Zhou GH, Wang HL, Zhu TY, Wang BQ, Yu ZN, Jing L, Wu LM, Zheng SS, Sun JH   +15 more
doaj  

Nicotinamide N‐methyltransferase promotes drug resistance in lung cancer, as revealed by nascent proteomic profiling

Molecular Oncology, EarlyView.
AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou, Zhen Wang, Fei Yang, Xiao Han, Lei Li, Huadong Liu   +5 more
wiley   +1 more source

Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation

Frontiers in Cellular Neuroscience, 2018
Microglia, the resident immune cells of the brain, have multiple functions in physiological and pathological conditions, including Alzheimer’s disease (AD).
Alejandro Carrillo-Jimenez, Alejandro Carrillo-Jimenez, Mar Puigdellívol, Anna Vilalta, Jose Luis Venero, Jose Luis Venero, Guy Charles Brown, Peter StGeorge-Hyslop, Miguel Angel Burguillos   +8 more
doaj   +1 more source