Results 41 to 50 of about 932,327 (388)
Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells. [PDF]
, 2015 The treatment of myocardial infarction is a major challenge in medicine due to the inability of heart tissue to regenerate. Direct reprogramming of endogenous cardiac fibroblasts into functional cardiomyocytes via the delivery of transcription factor ...
Kim, Hyun Jin +5 more
core +3 more sources
Plate-based transfection and culturing technique for genetic manipulation of
Malaria Journal, 2012 Genetic manipulation of malaria parasites remains an inefficient, time-consuming and resource-intensive process. Presented here is a set of methods for 96-well plate-based transfection and culture that improve the efficiency of genetic manipulation of ...
Caro Florence +2 more
doaj +1 more source
Nature Communications, 2018 Effective transfection of genetic molecules such as DNA usually relies on vectors that can reversibly uptake and release these molecules, and protect them from digestion by nuclease.
Shuang Peng +9 more
semanticscholar +1 more source
MicroRNAs delivery into human cells grown on 3D-printed PLA scaffolds coated with a novel fluorescent PAMAM dendrimer for biomedical applications [PDF]
, 2018 Many advanced synthetic, natural, degradable or non-degradable materials have been employed to create scaffolds for cell culture for biomedical or tissue engineering applications. One of the most versatile material is poly-lactide (PLA), commonly used as
Abbaszadeh, Zeinab +7 more
core +2 more sources
Functional differences between protamine preparations for the transfection of mRNA
Drug Delivery, 2020 Protamine is a natural cationic peptide mixture used as a drug for the neutralization of heparin and in formulations of slow-release insulin. In addition, Protamine can be used for the stabilization and delivery of nucleic acids (antisense, small ...
Natalia Teresa Jarzebska +7 more
doaj +1 more source
pH-Sensitive Chitosan–Heparin Nanoparticles for Effective Delivery of Genetic Drugs into Epithelial Cells [PDF]
, 2019 Chitosan has been extensively studied as a genetic drug delivery platform. However, its efficiency is limited by the strength of DNA and RNA binding.
Korzhikov-Vlakh, Viktor +7 more
core +1 more source
Molecules, 2018 Non-viral vector-mediated transfection is a core technique for in vitro screening of oligonucleotides. Despite the growing interests in the development of oliogonucleotide-based drug molecules in recent years, a comprehensive comparison of the ...
Tao Wang +3 more
semanticscholar +1 more source
Molecular Therapy: Nucleic Acids, 2017 RNA interference (RNAi)-based strategies that mediate the specific knockdown of target genes by administration of small interfering RNAs (siRNAs) could be applied for treatment of presently incurable neurodegenerative diseases such as Parkinson’s disease.
Christin Helmschrodt +9 more
doaj +1 more source
Nucleofection as an Efficient Method for Alpha TC1-6 Cell Line Transfection
Applied Sciences, 2022 An efficient transfection is a crucial step for the introduction of epigenetic modification in host cells, and there is a need for an optimized transfection process for individual model systems separately.
Marija Đorđević +11 more
doaj +1 more source
Nature Communications, 2019 Current therapies for most neurodegenerative disorders are only symptomatic in nature and do not change the course of the disease. Gene therapy plays an important role in disease modifying therapeutic strategies.
Shuai Liu +11 more
semanticscholar +1 more source