Results 11 to 20 of about 70,182 (263)

A P. falciparum NF54 Reporter Line Expressing mCherry-Luciferase in Gametocytes, Sporozoites, and Liver-Stages

Frontiers in Cellular and Infection Microbiology, 2019
Transgenic malaria parasites expressing fluorescent and bioluminescent proteins are valuable tools to interrogate malaria-parasite biology and to evaluate drugs and vaccines. Using CRISPR/Cas9 methodology a transgenic Plasmodium falciparum (Pf) NF54 line
Catherin Marin-Mogollon, Ahmed M. Salman, Karin M. J. Koolen, Judith M. Bolscher, Fiona J. A. van Pul, Shinya Miyazaki, Takashi Imai, Takashi Imai, Ahmad Syibli Othman, Ahmad Syibli Othman, Jai Ramesar, Geert-Jan van Gemert, Hans Kroeze, Severine Chevalley-Maurel, Blandine Franke-Fayard, Robert W. Sauerwein, Robert W. Sauerwein, Adrian V. S. Hill, Koen J. Dechering, Chris J. Janse, Shahid M. Khan   +20 more
doaj   +1 more source

DNA templates with blocked long 3ʹ end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci

G3: Genes, Genomes, Genetics, 2021
Knock-in of large transgenes by Cas9-mediated homology-directed repair (HDR) is an extremely inefficient process. Although the use of single-stranded oligonucleotides (ssODN) as an HDR donor has improved the integration of smaller transgenes, they do not
Saptaparni Bandyopadhyay, Joseph Douglass, Sebastian Kapell, Nazimuddin Khan, Fabiana Feitosa-Suntheimer, Jenny A Klein, Jasmine Temple, Jayce Brown-Culbertson, Alexander H Tavares, Mohsan Saeed, Nelson C Lau   +10 more
doaj   +1 more source

DNA Damage and Augmented Oxidative Stress in Bone Marrow Mononuclear Cells from Angiotensin-Dependent Hypertensive Mice

International Journal of Hypertension, 2013
It has been proposed that the nonhemodynamic effects of angiotensin II are important for the damage observed in the two-kidney, one-clip (2K1C) renovascular hypertension model.
Bianca P. Campagnaro, Clarissa L. Tonini, Breno V. Nogueira, Dulce E. Casarini, Elisardo C. Vasquez, Silvana S. Meyrelles   +5 more
doaj   +1 more source

Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells

Journal of Biological Engineering, 2023
Background Human mesenchymal stem cells (hMSCs) are being researched for cell-based therapies due to a host of unique properties, however, genetic modification of hMSCs, accomplished through nonviral gene delivery, could greatly advance their therapeutic
Tyler Kozisek, Luke Samuelson, Andrew Hamann, Angela K. Pannier   +3 more
doaj   +1 more source

Analysis of the transcriptional activity of model piggyBac transgenes stably integrated into different loci of the genome of CHO cells in the absence of selection pressure

Вавиловский журнал генетики и селекции, 2023
CHO cells are most commonly used for the synthesis of recombinant proteins in biopharmaceutical production. When stable producer cell lines are obtained, the locus of transgene integration into the genome has a great influence on the level of its ...
L. A. Yarinich, A. A. Ogienko, A. V. Pindyurin, E. S. Omelina   +3 more
doaj   +1 more source

When crop transgenes wander in California, should we worry?

California Agriculture, 2006
The movement of transgenes into populations for which they are not intended remains a primary concern for genetically engineered crops. Such gene flow in itself is not a risk.
Norman Ellstrand
doaj   +2 more sources

A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants

Frontiers in Plant Science, 2019
Advances in plant biotechnology provide various means to improve crop productivity and greatly contributing to sustainable agriculture. A significant advance in plant biotechnology has been the availability of novel synthetic promoters for precise ...
Sajid Ali, Won-Chan Kim
doaj   +1 more source

Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus. [PDF]

PLoS Pathogens, 2012
Functional studies will facilitate characterization of role and essentiality of newly available genome sequences of the human schistosomes, Schistosoma mansoni, S. japonicum and S. haematobium.
Gabriel Rinaldi, Sabine E Eckert, Isheng J Tsai, Sutas Suttiprapa, Kristine J Kines, José F Tort, Victoria H Mann, Daniel J Turner, Matthew Berriman, Paul J Brindley   +9 more
doaj   +1 more source

Expression of RNA-interference/antisense transgenes by the cognate promoters of target genes is a better gene-silencing strategy to study gene functions in rice. [PDF]

PLoS ONE, 2011
Antisense and RNA interference (RNAi)-mediated gene silencing systems are powerful reverse genetic methods for studying gene function. Most RNAi and antisense experiments used constitutive promoters to drive the expression of RNAi/antisense transgenes ...
Jing Li, Dagang Jiang, Hai Zhou, Feng Li, Jiawei Yang, Laifa Hong, Xiao Fu, Zhibin Li, Zhenlan Liu, Jianming Li, Chuxiong Zhuang   +10 more
doaj   +1 more source

Rethinking the Risk Management Process for Genetically Engineered Crop Varieties in Small-scale, Traditionally Based Agriculture

Ecology and Society, 2005
Proponents of genetically engineered (GE) crops often assume that the risk management used in the industrial world is appropriate for small-scale, traditionally based agriculture in the Third World. Opponents of GE crops often assume that risk management
David A. Cleveland, Daniela Soleri
doaj   +1 more source