Results 11 to 20 of about 84,438 (207)

When crop transgenes wander in California, should we worry? [PDF]

California Agriculture, 2006
The movement of transgenes into populations for which they are not intended remains a primary concern for genetically engineered crops. Such gene flow in itself is not a risk.
Norman Ellstrand
doaj   +3 more sources

Transgenes are revolutionizing crop production

California Agriculture, 2000
The ability to cut and join DNA to create a new molecule and to insert into the crop plant the new DNA molecule as a new gene, a “transgene,” forms the basis of the most revolutionary crop improvement technology of the 20th century.
G Bruening
doaj   +3 more sources

Contrasting epigenetic control of transgenes and endogenous genes promotes post-transcriptional transgene silencing in Arabidopsis

Nature Communications, 2021
Accumulating evidences point to a discrepancy in the epigenetic behaviour of transgenes and endogenous genes. Here, via characterization of mutants impaired in histone demethylases JMJ14 and IBM1, the authors show that transgenes and endogenous genes are
Nicolas Butel, Agnès Yu, Ivan Le Masson, Filipe Borges, Taline Elmayan, Christelle Taochy, Nial R. Gursanscky, Jiangling Cao, Shengnan Bi, Anne Sawyer, Bernard J. Carroll, Hervé Vaucheret   +11 more
doaj   +2 more sources

Genomic integration of transgenes using UV irradiation in Pristionchus pacificus [PDF]

, 2022
Transgenes are widely used throughout molecular biology for numerous applications. In Caenorhabditis elegans, stable transgenes are usually generated by microinjection into the germline establishing extrachromosomal arrays.
Eren, G., Han, Z., Lightfoot, J. ; https://orcid.org/, Han, Ziduan, Lightfoot, James W, Lightfoot, J., Eren, Güniz Göze, Roca, Marianne, Roca, M. ; https://orcid.org/, Roca, M., Eren, G. ; https://orcid.org/   +10 more
core   +1 more source

Stable transgenes bear fruit

, 2008
Analysis of the transgenic papaya genome sequence suggests that transgenes generally stay put following integration and can achieve stable expression level from generation to generation.
Kohli A, Christou P
core   +5 more sources

DNA templates with blocked long 3ʹ end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci

G3: Genes, Genomes, Genetics, 2021
Knock-in of large transgenes by Cas9-mediated homology-directed repair (HDR) is an extremely inefficient process. Although the use of single-stranded oligonucleotides (ssODN) as an HDR donor has improved the integration of smaller transgenes, they do not
Saptaparni Bandyopadhyay, Joseph Douglass, Sebastian Kapell, Nazimuddin Khan, Fabiana Feitosa-Suntheimer, Jenny A Klein, Jasmine Temple, Jayce Brown-Culbertson, Alexander H Tavares, Mohsan Saeed, Nelson C Lau   +10 more
doaj   +1 more source

DNA Damage and Augmented Oxidative Stress in Bone Marrow Mononuclear Cells from Angiotensin-Dependent Hypertensive Mice

International Journal of Hypertension, 2013
It has been proposed that the nonhemodynamic effects of angiotensin II are important for the damage observed in the two-kidney, one-clip (2K1C) renovascular hypertension model.
Bianca P. Campagnaro, Clarissa L. Tonini, Breno V. Nogueira, Dulce E. Casarini, Elisardo C. Vasquez, Silvana S. Meyrelles   +5 more
doaj   +1 more source

Lentiviral manipulation of gene expression in human adult and embryonic stem cells [PDF]

, 2006
Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery.
Joanne Crossley, Crossley, J., Andrew Godfrey, Takeuchi, Y., Clements, M.O., Boshoff, C., Godfrey, A., Mark O. Clements, Sam J. Wilson, Wilson, S.J., Yasu Takeuchi, Chris Boshoff   +11 more
core   +1 more source

Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells

Journal of Biological Engineering, 2023
Background Human mesenchymal stem cells (hMSCs) are being researched for cell-based therapies due to a host of unique properties, however, genetic modification of hMSCs, accomplished through nonviral gene delivery, could greatly advance their therapeutic
Tyler Kozisek, Luke Samuelson, Andrew Hamann, Angela K. Pannier   +3 more
doaj   +1 more source

Analysis of the transcriptional activity of model piggyBac transgenes stably integrated into different loci of the genome of CHO cells in the absence of selection pressure

Вавиловский журнал генетики и селекции, 2023
CHO cells are most commonly used for the synthesis of recombinant proteins in biopharmaceutical production. When stable producer cell lines are obtained, the locus of transgene integration into the genome has a great influence on the level of its ...
L. A. Yarinich, A. A. Ogienko, A. V. Pindyurin, E. S. Omelina   +3 more
doaj   +1 more source