Results 11 to 20 of about 80,448 (304)

Local and Regional Dynamics of Native Maize Seed Lot Use by Small-Scale Producers and Their Impact on Transgene Presence in Three Mexican States

Plants, 2023
Mexico harbors over 50% of maize’s genetic diversity in the Americas. Native maize varieties are actively managed by small-scale producers within a diverse array of cultivation systems.
Mariana Ayala-Angulo, Edgar J. González, Carolina Ureta, José Luis Chávez-Servia, Emmanuel González-Ortega, Remy Vandame, Alejandro de Ávila-Bloomberg, Geovanni Martínez-Guerra, Said González-Díaz, Rosey Obet Ruíz-González, Prisciliano Diego-Flores, Elena R. Álvarez-Buylla, Alma Piñeyro-Nelson   +12 more
doaj   +1 more source

Transgene Design [PDF]

, 2010
Transgenics are powerful mouse models to understand the biological functions of genes. This chapter gives a short overview of the requirements and considerations in designing a transgene. In addition, potential important choices that have to be made in advance for the successful designing and generating a transgenic mouse model are discussed.
van de Sluis, Bart, Voncken, Jan Willem
openaire   +3 more sources

Monitoring the Efficacy of Oncolytic Viruses via Gene Expression

Frontiers in Oncology, 2017
With the recent success of oncolytic viruses in clinical trials, efforts toward improved monitoring of the viruses and their mechanism have intensified.
Ashley Ansel, Joshua P. Rosenzweig, Philip D. Zisman, Beni Gesundheit   +3 more
doaj   +1 more source

ESI mutagenesis: a one-step method for introducing mutations into bacterial artificial chromosomes

Life Science Alliance, 2021
A simple and efficient recombineering-based method for introducing point mutations into bacterial artificial chromosomes using an artificial intron cassette.
Arnaud Rondelet, Andrei Pozniakovsky, Devika Namboodiri, Richard Cardoso da Silva, Divya Singh, Marit Leuschner, Ina Poser, Andrea Ssykor, Julian Berlitz, Nadine Schmidt, Lea Röhder, Gerben Vader, Anthony A Hyman, Alexander W Bird   +13 more
doaj   +1 more source

A P. falciparum NF54 Reporter Line Expressing mCherry-Luciferase in Gametocytes, Sporozoites, and Liver-Stages

Frontiers in Cellular and Infection Microbiology, 2019
Transgenic malaria parasites expressing fluorescent and bioluminescent proteins are valuable tools to interrogate malaria-parasite biology and to evaluate drugs and vaccines. Using CRISPR/Cas9 methodology a transgenic Plasmodium falciparum (Pf) NF54 line
Catherin Marin-Mogollon, Ahmed M. Salman, Karin M. J. Koolen, Judith M. Bolscher, Fiona J. A. van Pul, Shinya Miyazaki, Takashi Imai, Takashi Imai, Ahmad Syibli Othman, Ahmad Syibli Othman, Jai Ramesar, Geert-Jan van Gemert, Hans Kroeze, Severine Chevalley-Maurel, Blandine Franke-Fayard, Robert W. Sauerwein, Robert W. Sauerwein, Adrian V. S. Hill, Koen J. Dechering, Chris J. Janse, Shahid M. Khan   +20 more
doaj   +1 more source

DNA templates with blocked long 3ʹ end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci

G3: Genes, Genomes, Genetics, 2021
Knock-in of large transgenes by Cas9-mediated homology-directed repair (HDR) is an extremely inefficient process. Although the use of single-stranded oligonucleotides (ssODN) as an HDR donor has improved the integration of smaller transgenes, they do not
Saptaparni Bandyopadhyay, Joseph Douglass, Sebastian Kapell, Nazimuddin Khan, Fabiana Feitosa-Suntheimer, Jenny A Klein, Jasmine Temple, Jayce Brown-Culbertson, Alexander H Tavares, Mohsan Saeed, Nelson C Lau   +10 more
doaj   +1 more source

Genomic integration of transgenes using UV irradiation in Pristionchus pacificus

, 2022
Transgenes are widely used throughout molecular biology for numerous applications. In Caenorhabditis elegans, stable transgenes are usually generated by microinjection into the germline establishing extrachromosomal arrays.
Eren, G., Han, Z., Lightfoot, J. ; https://orcid.org/, Han, Ziduan, Lightfoot, James W, Lightfoot, J., Eren, Güniz Göze, Roca, Marianne, Roca, M. ; https://orcid.org/, Roca, M., Eren, G. ; https://orcid.org/   +10 more
core   +1 more source

DNA Damage and Augmented Oxidative Stress in Bone Marrow Mononuclear Cells from Angiotensin-Dependent Hypertensive Mice

International Journal of Hypertension, 2013
It has been proposed that the nonhemodynamic effects of angiotensin II are important for the damage observed in the two-kidney, one-clip (2K1C) renovascular hypertension model.
Bianca P. Campagnaro, Clarissa L. Tonini, Breno V. Nogueira, Dulce E. Casarini, Elisardo C. Vasquez, Silvana S. Meyrelles   +5 more
doaj   +1 more source

Lentiviral manipulation of gene expression in human adult and embryonic stem cells [PDF]

, 2006
Human stem cells could revolutionize the field of medicine by providing a diverse range of cell types for tissue replacement therapies and drug discovery.
Joanne Crossley, Crossley, J., Andrew Godfrey, Takeuchi, Y., Clements, M.O., Boshoff, C., Godfrey, A., Mark O. Clements, Sam J. Wilson, Wilson, S.J., Yasu Takeuchi, Chris Boshoff   +11 more
core   +1 more source

Systematic comparison of nonviral gene delivery strategies for efficient co-expression of two transgenes in human mesenchymal stem cells

Journal of Biological Engineering, 2023
Background Human mesenchymal stem cells (hMSCs) are being researched for cell-based therapies due to a host of unique properties, however, genetic modification of hMSCs, accomplished through nonviral gene delivery, could greatly advance their therapeutic
Tyler Kozisek, Luke Samuelson, Andrew Hamann, Angela K. Pannier   +3 more
doaj   +1 more source