Results 31 to 40 of about 84,438 (207)

Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration

open access: yesFrontiers in Genome Editing, 2023
Increased understanding of plant genetics and the development of powerful and easier-to-use gene editing tools over the past century have revolutionized humankind’s ability to deliver precise genotypes in crops.
Zuzana Kocsisova, Viktoriya Coneva
doaj   +1 more source

Insertion of PATC-rich C. elegans introns into synthetic transgenes by golden-gate-based cloning [PDF]

open access: yes, 2021
Transgenes are particularly prone to epigenetic silencing in the C. elegans germline. Here, we describe a protocol to insert introns containing a class of non-coding DNA named Periodic An/Tn Clusters (PATCs) into synthetic transgenes.
Frøkjær-Jensen, Christian
core   +1 more source

New approaches for characterization of the genetic stability of vaccine cell lines

open access: yesHuman Vaccines & Immunotherapeutics, 2017
The genetic stability of cell lines is a critical analytical attribute required to demonstrate the quality of cells over time. During cell passage, mutations can arise in the genomic DNA, potentially leading to changes in the final vaccine product.
Siemon Ng, Lucy Gisonni-Lex, Ali Azizi
doaj   +1 more source

Transgenes used for Double RNAi.

open access: yes, 2019
Transgenes used for Double RNAi.
Kim S. McKim (243877)   +2 more
core   +1 more source

Chromosome-wide mechanisms to decouple gene expression from gene dose during sex-chromosome evolution

open access: yeseLife, 2016
Changes in chromosome number impair fitness by disrupting the balance of gene expression. Here we analyze mechanisms to compensate for changes in gene dose that accompanied the evolution of sex chromosomes from autosomes.
Bayly S Wheeler   +5 more
doaj   +1 more source

Combined transgene immortalized urothelial cells capable of reprogramming and hepatic differentiation

open access: yesBiochemistry and Biophysics Reports, 2022
Human primary cells, including urine-derived cells (UCs), are an excellent source for generation of pluripotent stem cells (iPSCs) to model disease. However, replicative senescence starts early and shortens the time window for generation of iPSCs.
Matthias Weiand   +4 more
doaj   +1 more source

Gene flow from weedy rice to T1c-19 transgenic rice stacked with cry1C*/bar genes and fitness of F1 hybrids

open access: yesFrontiers in Plant Science
IntroductionBidirectional gene flow via pollen between transgenic rice and weedy rice could occur in natural fields. Gene flow from transgenic rice to weedy rice has been confirmed in many studies, and thus results showed that F1 hybrids could persist in
Guang-Le Xie   +7 more
doaj   +1 more source

A built-in mechanism to mitigate the spread of insect-resistance and herbicide-tolerance transgenes into weedy rice populations. [PDF]

open access: yesPLoS ONE, 2012
BACKGROUND: The major challenge of cultivating genetically modified (GM) rice (Oryza sativa) at the commercial scale is to prevent the spread of transgenes from GM cultivated rice to its coexisting weedy rice (O. sativa f. spontanea).
Chengyi Liu   +5 more
doaj   +1 more source

Relação entre a presença de proteínas recombinantes de milho OGM e a frequência de fenótipos anormais nas variedades de milho nativo, na região Vales Centrais, Oaxaca, México [PDF]

open access: yes, 2012
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Curso de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2011México é o centro de origem e diversidade do milho e, portanto, seu cultivo tem grande
López, Flor Rivera
core  

Inheritance of GFP-Bt transgenes from

open access: yes, 2004
Transgenes from transgenic oilseed rape, Brassica napus (AACC genome), can introgress into populations of wild B. rapa (AA genome), but little is known about the long-term persistence of transgenes from different transformation events.
Matthew D. Halfhill   +6 more
core   +1 more source

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