Results 31 to 40 of about 118,253 (302)
New approaches for characterization of the genetic stability of vaccine cell lines
Human Vaccines & Immunotherapeutics, 2017 The genetic stability of cell lines is a critical analytical attribute required to demonstrate the quality of cells over time. During cell passage, mutations can arise in the genomic DNA, potentially leading to changes in the final vaccine product.
Siemon Ng, Lucy Gisonni-Lex, Ali Azizi
doaj +1 more source
Nature Communications, 2021 Accumulating evidences point to a discrepancy in the epigenetic behaviour of transgenes and endogenous genes. Here, via characterization of mutants impaired in histone demethylases JMJ14 and IBM1, the authors show that transgenes and endogenous genes are
Nicolas Butel +11 more
doaj +1 more source
Frontiers in Genome Editing, 2023 Increased understanding of plant genetics and the development of powerful and easier-to-use gene editing tools over the past century have revolutionized humankind’s ability to deliver precise genotypes in crops.
Zuzana Kocsisova, Viktoriya Coneva
doaj +1 more source
Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling [PDF]
, 2008 In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other ...
Appiah, A.A. +7 more
core +3 more sources
Effects of flanking sequences and cellular context on subcellular behavior and pathology of mutant HTT [PDF]
, 2020 Huntington’s disease (HD) is caused by an expansion of a poly glutamine (polyQ) stretch in the huntingtin protein (HTT) that is necessary to cause pathology and formation of HTT aggregates.
Agrawal, Namita +10 more
core
A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants
Frontiers in Plant Science, 2019 Advances in plant biotechnology provide various means to improve crop productivity and greatly contributing to sustainable agriculture. A significant advance in plant biotechnology has been the availability of novel synthetic promoters for precise ...
Sajid Ali, Won-Chan Kim
doaj +1 more source
Matrix metalloproteinase-20 over-expression is detrimental to enamel development: a Mus musculus model. [PDF]
PLoS ONE, 2014 Matrix metalloproteinase-20 (Mmp20) ablated mice have enamel that is thin and soft with an abnormal rod pattern that abrades from the underlying dentin.
Masashi Shin +8 more
doaj +1 more source
Measurement of Transgenes Copy Number in Wheat Plants Using Droplet Digital PCR
Bio-Protocol, 2022 Genetic transformation is a powerful method for the investigation of gene function and improvement of crop plants. The transgenes copy number in the transgenic line is involved in gene expression level and phenotypes.
Peng Liu +4 more
doaj +1 more source
eLife, 2016 Changes in chromosome number impair fitness by disrupting the balance of gene expression. Here we analyze mechanisms to compensate for changes in gene dose that accompanied the evolution of sex chromosomes from autosomes.
Bayly S Wheeler +5 more
doaj +1 more source
Biochemistry and Biophysics Reports, 2022 Human primary cells, including urine-derived cells (UCs), are an excellent source for generation of pluripotent stem cells (iPSCs) to model disease. However, replicative senescence starts early and shortens the time window for generation of iPSCs.
Matthias Weiand +4 more
doaj +1 more source