Results 21 to 30 of about 80,448 (304)
Вавиловский журнал генетики и селекции, 2023 CHO cells are most commonly used for the synthesis of recombinant proteins in biopharmaceutical production. When stable producer cell lines are obtained, the locus of transgene integration into the genome has a great influence on the level of its ...
L. A. Yarinich +3 more
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A Fruitful Decade Using Synthetic Promoters in the Improvement of Transgenic Plants
Frontiers in Plant Science, 2019 Advances in plant biotechnology provide various means to improve crop productivity and greatly contributing to sustainable agriculture. A significant advance in plant biotechnology has been the availability of novel synthetic promoters for precise ...
Sajid Ali, Won-Chan Kim
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Germline transgenesis and insertional mutagenesis in Schistosoma mansoni mediated by murine leukemia virus. [PDF]
PLoS Pathogens, 2012 Functional studies will facilitate characterization of role and essentiality of newly available genome sequences of the human schistosomes, Schistosoma mansoni, S. japonicum and S. haematobium.
Gabriel Rinaldi +9 more
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Expression of RNA-interference/antisense transgenes by the cognate promoters of target genes is a better gene-silencing strategy to study gene functions in rice. [PDF]
PLoS ONE, 2011 Antisense and RNA interference (RNAi)-mediated gene silencing systems are powerful reverse genetic methods for studying gene function. Most RNAi and antisense experiments used constitutive promoters to drive the expression of RNAi/antisense transgenes ...
Jing Li +10 more
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Ecology and Society, 2005 Proponents of genetically engineered (GE) crops often assume that the risk management used in the industrial world is appropriate for small-scale, traditionally based agriculture in the Third World. Opponents of GE crops often assume that risk management
David A. Cleveland, Daniela Soleri
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Multi-locus assortment (MLA) for transgene dispersal and elimination in mosquito populations. [PDF]
PLoS ONE, 2009 BACKGROUND:Replacement of wild-type mosquito populations with genetically modified versions is being explored as a potential strategy to control vector-borne diseases.
Jason L Rasgon
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Nature Communications, 2022 Long hairpin RNA (hpRNA) transgenes are the most widely used RNAi technology in plants, but are potentially subject to self-induced transcriptional silencing.
Daai Zhang +7 more
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Nonviral Gold Nanoparticle-Mediated Delivery of CRISPR-Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells. [PDF]
Adv Nanobiomed ResCRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Cunningham RA +8 more
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Measurement of Transgenes Copy Number in Wheat Plants Using Droplet Digital PCR
Bio-Protocol, 2022 Genetic transformation is a powerful method for the investigation of gene function and improvement of crop plants. The transgenes copy number in the transgenic line is involved in gene expression level and phenotypes.
Peng Liu +4 more
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Synthetic mammalian transgene negative autoregulation
Molecular Systems Biology, 2013 Biological networks contain overrepresented small‐scale topologies, typically called motifs. A frequently appearing motif is the transcriptional negative‐feedback loop, where a gene product represses its own transcription.
Vinay Shimoga +4 more
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