Results 51 to 60 of about 80,448 (304)

Transgenes and their contributions to epigenetic research

, 2013
Shortly after gene transfer technologies had been established for different plant species, the first reports emerged about transgenes showing unexpected segregation patterns due to unstable expression.
Meyer, P
core   +1 more source

Insertion of PATC-rich C. elegans introns into synthetic transgenes by golden-gate-based cloning

, 2021
Transgenes are particularly prone to epigenetic silencing in the C. elegans germline. Here, we describe a protocol to insert introns containing a class of non-coding DNA named Periodic An/Tn Clusters (PATCs) into synthetic transgenes.
Frøkjær-Jensen, Christian
core   +1 more source

Microenvironmental Reprogramming by 3D Anisotropic Cardiac Extracellular Matrix Induces Nuclear Remodeling and Epigenetic Maturation of Chemically Induced Cardiomyocytes

Advanced Functional Materials, EarlyView.
A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo, Mi Jeong Lee, Hyun Wook Kang, Seonhee Byeon, Soo‐Kyoung Choi, Nakwon Choi, Seung‐Woo Cho, Yoonhee Jin   +7 more
wiley   +1 more source

Zasp52 transgenes localize to IFM Z-discs.

, 2016
(A) Cartoon of Zasp52 transgenes. PK and PR refer to the Zasp52 isoform the transgenes are based on (see FlyBase). Flag, Flag-tag; FSH, Flag/Streptavidin/His-tag; ZM, Zasp-like motif; a.a., amino acids.
Kuo An Liao (386444), Nicanor González-Morales (772830), Frieder Schöck (386446)   +2 more
core   +1 more source

Adipocyte‐Derived Leptolin Enhances Energy Expenditure and Prevents Obesity

Advanced Science, EarlyView.
We identified a novel adipokine, which we named leptolin. In humans, leptolin levels in white adipose tissue were positively correlated with exercise and negatively associated with body mass index. We observed elevated leptolin in serum from athletes and lower leptolin in serum from obese individuals.
Jiarui Liu, Bingwei Wang, Zhijie Su, Xiaoxv Han, Miao He, Yun Zhao, Yujia Hou, Daotong Li, Weiguang Zhang, Lihua Qin, Ke Wang, Yanchun Li, Yi Yan, Siwang Yu, Xiaoshuai Huang, Tairan Yuwen, Jianyuan Luo, Ruimao Zheng   +17 more
wiley   +1 more source

A Rare Allele of ST5 From Wild Rice Enhances Salt Tolerance in Rice

Advanced Science, EarlyView.
A novel salt‐tolerance gene ST5W, characterized by a unique 36‐bp promoter insertion, is found exclusively in a small subset of Oryza rufipogon and is absent in cultivated rice. Field trials confirm ST5W significantly enhances rice yield across diverse genetic backgrounds under saline conditions.
Meng Xing, Jingfen Huang, Qiaoling Yuan, Ziyi Yang, Yanyan Wang, Mingchao Zhao, Yamin Nie, Rui Xu, Hongge Qian, Wenxi Chen, Qiaoling Zhang, Qi Du, Leiyue Geng, Yapeng Li, Ziyi Chen, Shizhuang Wang, Like Lou, Haiyuan Peng, Chongke Zheng, Xianzhi Xie, Xiaoming Zheng, Lifang Zhang, Lianguang Shang, Jiaqiang Sun, Qian Qian, Qingwen Yang, Weihua Qiao   +26 more
wiley   +1 more source

High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes

BMC Genomics, 2022
Background Genetic engineering of crop plants has been successful in transferring traits into elite lines beyond what can be achieved with breeding techniques.
Brianne Edwards, Eli D. Hornstein, Nathan J. Wilson, Heike Sederoff   +3 more
doaj   +1 more source

Single-copy insertion of transgenes in Caenorhabditis elegans

, 2008
Udgivelsesdato: 2008-NovAt present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA.
Christian Frøkjær-Jensen, Frøkjaer-Jensen, Christian, Olesen, Søren-Peter, Søren-Peter Olesen, Thummel, Jason M, Jorgensen, Erik M, Davis, M Wayne, Christopher E Hopkins, Erik M Jorgensen, Grunnet, Morten, M Wayne Davis, Morten Grunnet, Hopkins, Christopher E, Newman, Blake J, Blake J Newman, Jason M Thummel   +15 more
core   +1 more source

A Toolkit for Targeted Neuromodulation of Striatal Direct Pathway Neurons Rescues Parkinsonian Motor Deficits in Mice

Advanced Science, EarlyView.
An adeno‐associated virus (AAV) toolkit enables selective anatomical and functional targeting of striatal D1‐MSNs through retrograde transduction. Enhanced capsids and engineered enhancers drive robust transgene expression across murine and primate models.
Zexuan Hong, Yujing Zhang, Junjiao Zhang, Hanhe Liu, Qiwei Liu, Yanglei Li, Lixin Yang, Lixia Li, Zhongjie Liu, Zhen Yuan, Zhonghua Lu, Yefei Chen, Yuantao Li, Yuwu Jiang, Taian Liu   +14 more
wiley   +1 more source

mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish

, 2011
Macrophages and neutrophils play important roles during the innate immune response, phagocytosing invading microbes and delivering antimicrobial compounds to the site of injury.
Felix Ellett, Luke Pase, John W. Hayman, Alex Andrianopoulos, Graham J. Lieschke   +4 more
core   +1 more source