Results 51 to 60 of about 84,438 (207)
CellsThe stable and safe integration of exogenous DNA into the genome is crucial to both genetic engineering and gene therapy. Traditional transgenesis approaches, such as those using retroviral vectors, result in random genomic integration, posing the risk ...
Amer Ahmed +9 more
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Frontiers in Neural Circuits, 2013 The fruit fly Drosophila melanogaster represents a key model organism for analyzing how neuronal circuits regulate behavior. The mushroom body in the central brain is a particularly prominent brain region that has been intensely studied in several insect
Ulrike ePech +7 more
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Genetically Modified Plants in Agriculture
BiologyGenetically modified (GM) plants have revolutionized agriculture for more than three decades. The production of a GM plants is a complex, multi-stage process. Several key methods are available for generating GM plants. The choice of transformation method
Anna A. Ogienko +2 more
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A multiplexable TALE-based binary expression system for in vivo cellular interaction studies
Nature Communications, 2017 Binary expression systems enable researchers to deliver loss-of-function or gain-of-function transgenes with spatial-temporal resolution in vivo.
Markus Toegel +6 more
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Expression of transgenes targeted to the Gt(ROSA)26Sor locus is orientation dependent. [PDF]
PLoS ONE, 2006 Targeting transgenes to a chosen location in the genome has a number of advantages. A single copy of the DNA construct can be inserted by targeting into regions of chromatin that allow the desired developmental and tissue-specific expression of the ...
Douglas Strathdee +2 more
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mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish
, 2011 Macrophages and neutrophils play important roles during the innate immune response, phagocytosing invading microbes and delivering antimicrobial compounds to the site of injury.
Felix Ellett +4 more
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Maize Transgenes Containing Zein Promoters are Regulated by opaque2 [PDF]
, 2011 Understanding epistatic interactions between transgenes and native genes is critical for predicting the response of transgenes to different genetic backgrounds and environments.
Scott, M. Paul +2 more
core
Single-copy insertion of transgenes in Caenorhabditis elegans
, 2008 Udgivelsesdato: 2008-NovAt present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA.
Christian Frøkjær-Jensen +15 more
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5′ Transgenes drive leaky expression of 3′ transgenes in Cre-inducible bi-cistronic vectors
Molecular Therapy: Methods & Clinical DevelopmentMolecular cloning techniques enabling contemporaneous expression of two or more protein-coding sequences provide an invaluable tool for understanding the molecular regulation of cellular functions.
Yasuyuki Osanai +9 more
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