Results 51 to 60 of about 70,182 (263)

Encoded Cell‐Material Interactions to Reroute Cytokine Signaling for Regenerative Medicine

Advanced Healthcare Materials, EarlyView.
We present native MATRIX (Material Activated To Regulate Inducible gene eXpression), a co‐engineered material‐cell platform. Surfaces functionalized with antibodies for endogenous soluble ligands (e.g., IL‐1β or IL‐6) capture and immobilize ligands for presentation to a cognate, engineered synthetic Notch receptor, releasing a transcription factor (TF)
Zachary M. Eidman, Jhanvi Sharma, Joanne C. Lee, Nicholas F. Schulze, Hannah J. Brien, Kevin C. Corn, Marjan Rafat, Jonathan M. Brunger   +7 more
wiley   +1 more source

A multiplexable TALE-based binary expression system for in vivo cellular interaction studies

Nature Communications, 2017
Binary expression systems enable researchers to deliver loss-of-function or gain-of-function transgenes with spatial-temporal resolution in vivo.
Markus Toegel, Ghows Azzam, Eunice Y. Lee, David J. H. F. Knapp, Ying Tan, Ming Fa, Tudor A. Fulga   +6 more
doaj   +1 more source

Nanomaterial Strategies for Pulmonary Delivery of Immunotherapeutics in Lung Cancer Treatment

Advanced Healthcare Materials, EarlyView.
Inhalable immunotherapeutic nanomedicines enable organ‐selective immune modulation by overcoming pulmonary delivery barriers and concentrating therapy within lung tumors. This Review defines how nanomaterial properties govern airway deposition, retention, cellular partitioning, and immune activation across vaccines, checkpoint blockade, STING agonists,
Han Zhang, Wei Tang
wiley   +1 more source

Expression of transgenes targeted to the Gt(ROSA)26Sor locus is orientation dependent. [PDF]

PLoS ONE, 2006
Targeting transgenes to a chosen location in the genome has a number of advantages. A single copy of the DNA construct can be inserted by targeting into regions of chromatin that allow the desired developmental and tissue-specific expression of the ...
Douglas Strathdee, Helen Ibbotson, Seth G N Grant   +2 more
doaj   +1 more source

Inhibition of VEGF‐Induced Angiogenesis and Vascular Leakage by Bicistronic Co‐Expression of Aflibercept and COMP‐Ang1

Advanced Healthcare Materials, EarlyView.
A bicistronic rAAV8 vector encoding Aflibercept (Afb) and COMP‐cAng1 simultaneously coordinates VEGF clearance and Tie2‐mediated vessel maturation. In a 3D angiogenesis‐on‐a‐chip model, rAAV8‐Afb/cAng1 effectively reverses pathological barrier breakdown under clinical disease‐mimicking challenges.
Bong‐Kyu Kim, Jaehong Min, Sang‐Hee Park, Subeen Shin, Sangbin Yu, Jeong‐Eun Park, Sujin Kim, Wooju Park, Jihoon Ko, Sunjong Kwon   +9 more
wiley   +1 more source

Transgenes are revolutionizing crop production

California Agriculture, 2000
The ability to cut and join DNA to create a new molecule and to insert into the crop plant the new DNA molecule as a new gene, a “transgene,” forms the basis of the most revolutionary crop improvement technology of the 20th century.
G Bruening
doaj   +2 more sources

Cellularized Skin Substitute Bioengineering for Regenerative Medicine: Cell Sources, Culture Strategies, and Transition Toward Defined, Xeno‐Free Culture Systems

Advanced Healthcare Materials, EarlyView.
Advances in cell sources, bioengineering, and manufacturing are reshaping the design of skin substitutes. This review highlights emerging strategies driving skin tissue engineering and discusses their potential to enable safer, reproducible, and clinically accessible skin substitutes for regenerative medicine.
Gilles Lemaître, Marina Trouillas, Tatiana Vinasco‐Sandoval, Cécile Giordano, Alexandre G. Lellouch, Walid Rachidi, Jean‐Jacques Lataillade, Nicolas O. Fortunel   +7 more
wiley   +1 more source

5′ Transgenes drive leaky expression of 3′ transgenes in Cre-inducible bi-cistronic vectors

Molecular Therapy: Methods & Clinical Development
Molecular cloning techniques enabling contemporaneous expression of two or more protein-coding sequences provide an invaluable tool for understanding the molecular regulation of cellular functions.
Yasuyuki Osanai, Yao Lulu Xing, Shinya Mochizuki, Kenta Kobayashi, Jihane Homman-Ludiye, Amali Cooray, Jasmine Poh, Ayumu Inutsuka, Nobuhiko Ohno, Tobias D. Merson   +9 more
doaj   +1 more source

Membrane‐Bound Multimodal Plasmonic Transducers for Noninvasive, In Situ Monitoring and Control of CAR T Cells Against Heterogeneous Solid Tumors

Advanced Materials, EarlyView.
Membrane‐bound multimodal transducers enable longitudinal ultrasound‐guided photoacoustic monitoring and in situ thermal control of CAR T cells in heterogeneous solid tumors. Imaging correlates T cell trafficking with tumor response, distinguishing responders from nonresponders.
Myeongsoo Kim, Ali Zamat, Melissa Cadena, Chloé Thiveaud, Riya Sen, Jeungyoon Lee, Elif Kulaksizoglu, S. Abbey Oliver, Don VanderLaan, Sydney Fábrega, Zizhen Zha, Kelsey P. Kubelick, Jinhwan Kim, Gabriel A. Kwong, Stanislav Y. Emelianov   +14 more
wiley   +1 more source

Transgenic livestock

Bioscientifica Proceedings, 2019
Single genes can now be added routinely to the genome of mice by molecular manipulation as simple Mendelian dominants; this complements the normal process of reproduction to give 'transgenic' animals. Success in ruminants is limited to a few examples in sheep and although gene expression has yet to be documented, there is every reason to expect that it
J P, Simons, R B, Land
openaire   +2 more sources