Results 71 to 80 of about 80,448 (304)
A multiplexable TALE-based binary expression system for in vivo cellular interaction studies
Nature Communications, 2017 Binary expression systems enable researchers to deliver loss-of-function or gain-of-function transgenes with spatial-temporal resolution in vivo.
Markus Toegel +6 more
doaj +1 more source
Expression capability of UAS-endoA* transgenes.
, 2013 A, Western blots of extracts from late-stage embryos, probed simultaneously with anti-EndoA and anti-Elav primary antibodies. Shown are genotypes without transgenes (w1118 and elav-GAL4), EndoAΔ4 null mutants, and null mutants carrying the indicated ...
Klemens Wild (366673) +5 more
core +1 more source
Stability and inheritance of endosperm-specific expression of two transgenes in progeny from crossing independently transformed barley plants [PDF]
, 2009 To study stability and inheritance of two different transgenes in barley, we crossed a homozygous T8 plant, having uidA (or gus) driven by the barley endosperm-specific B1-hordein promoter (localized in the near centromeric region of chromosome 7H) with ...
Yu, Xiao-Hong +7 more
core +1 more source
Advanced Science, EarlyView.Understanding how glomeruli, the kidney's filtration units, function in intact tissue remains challenging. Glomage enables rapid 3D imaging and quantitative analysis of entire glomeruli from zebrafish larvae and mice. This approach allows scalable cell quantification and detection of structural changes, advancing kidney disease research and ...
Maximilian Schindler +4 more
wiley +1 more source
Expression of transgenes targeted to the Gt(ROSA)26Sor locus is orientation dependent. [PDF]
PLoS ONE, 2006 Targeting transgenes to a chosen location in the genome has a number of advantages. A single copy of the DNA construct can be inserted by targeting into regions of chromatin that allow the desired developmental and tissue-specific expression of the ...
Douglas Strathdee +2 more
doaj +1 more source
Interaction of recipient genome and transgenes in eukaryotes. Integrative transgenes
, 2002 Представлено огляд досліджень з проблеми трансгенозу в еукаріотів. Розглядаються питання, пов'язані з формами існування трансгенів, механізмами їхньої інтеграції та модифікації.
Крисан, К.В. +1 more
core +1 more source
The Effects of Transcription Directions of Transgenes and the gypsy Insulators on the Transcript Levels of Transgenes in Transgenic Arabidopsis [PDF]
, 2017 Manipulation of a single abiotic stress-related gene could improve plant performance under abiotic stress conditions. To simultaneously increase plant tolerance to multiple stresses, it is usually required to overexpress two (or more) genes in transgenic
Esmaeili, Nardana +17 more
core +1 more source
Optimization of B Cell Responses in Human Immune System Mice Through Organoid Based Screening
Advanced Science, EarlyView.Human immune system (HIS) mice show limited B cell responses to immunization. Using HIS mouse spleen organoids, this study identifies cytokine combinations and innate immune signals that drive human B cell expansion, differentiation, and class‐switch. Applying these signals in vivo through temporal separation enables antigen‐specific IgG responses and ...
Haiqiao Sun +6 more
wiley +1 more source
, 2015 PCR genotyping was performed for all four transgenes as described in materials and methods. Sizes of the amplified products obtained are: 240 bp for Hif-1α (wild type); 274 bp for Hif-1αflox/flox; 410 bp for Hif-2α (wild type); 444bp for Hif-2αflox/flox;
Krista K. Greenwood (801688) +11 more
core +1 more source