Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs. [PDF]
BiomoleculesHormigo D +4 more
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A multi-dimensional engineering strategy integrating computational design, self-cyclization and whole cell biocatalysis for efficient epilactose production. [PDF]
Food Chem XSun S +8 more
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Specificity of glycosyl donors is a critical issue in transglycosylation of stevioside, the main methodology to improve edulcorant quality of stevioside. The most popular glucanotransferase applied in this reaction is cyclodextrin glucanotransferase (CGTase) that catalyses cyclisation, coupling, hydrolysis and disproportionation simultaneously; which ...
Yongmei Xia
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Fluorescence detection of the transglycosylation activity of amylosucrase
Analytical Biochemistry, 2017The purpose of this study was to investigate the novel fluorescence-based assay for the transglycosylation activity of amylosucrase (ASase). The transglycosylation activity of ASase from Deinococcus geothermalis (DGAS), ASase from Neisseria polysaccharea (NPAS), and DGAS-B (chimeric ASase wherein the B domain from DGAS was exchanged with the B domain ...
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Loop Protein Engineering for Improved Transglycosylation Activity of a β-N-Acetylhexosaminidase [PDF]
ChemBioChem, 2018 Certain enzymes of the glycoside hydrolase family 20 (GH20) exert transglycosylation activity and catalyze the transfer of β‐N‐acetylglucosamine (GlcNAc) from a chitobiose donor to lactose to produce lacto‐N‐triose II (LNT2), a key human milk ...
Shariza B Jamek +2 more
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An Enzymatic Transglycosylation of Purine Bases
Nucleosides, Nucleotides & Nucleic Acids, 2007An enzymatic transglycosylation of purine heterocyclic bases employing readily available natural nucleosides or sugar-modified nucleosides as donors of the pentofuranose fragment and recombinant nucleoside phosphorylases as biocatalysts has been investigated.
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Transglycosylation of N-acylethanolamines [PDF]
N-acylethanolamines (NAEs), including N-arachidonoylethanolamine (anandamide, AEA), N-palmitoylethanolamine (PEA) and N-oleoylethanolamine (OEA), are signaling lipids that play an important role in different physiological process.
Stevens, A.F.
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Enzymatic transglycosylation of xylose using a glycosynthase
Carbohydrate Research, 2005The application of the hyperactive glycosynthase derived from Agrobacterium sp. beta-glucosidase (AbgE358G-2F6) to the synthesis of xylo-oligosaccharides by using alpha-D-xylopyranosyl fluoride as donor represents the first successful use of glycosynthase technology for xylosyl transfer.
Young-Wan, Kim +2 more
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Transglycosylation of Guanine Nucleosides
Nucleosides and Nucleotides, 1987Abstract Tetraacetylguanosine was converted into fully acetylated derivatives of acyclovir and 9-β-D-xylofuranosyl guanine in a novel transglyco-sylation ...
Jeratzy Boryski, Bozenna Golankiewicz
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An Aspergillus nidulans β-mannanase with high transglycosylation capacity revealed through comparative studies within glycosidase family 5 [PDF]
Applied Microbiology and Biotechnology, 2014 β-Mannanases are involved in the conversion and modification of mannan-based saccharides. Using a retaining mechanism, they can, in addition to hydrolysis, also potentially perform transglycosylation reactions, synthesizing new glyco-conjugates ...
Henrik Stålbrand +2 more
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