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An Aspergillus nidulans β-mannanase with high transglycosylation capacity revealed through comparative studies within glycosidase family 5 [PDF]

Applied Microbiology and Biotechnology, 2014
β-Mannanases are involved in the conversion and modification of mannan-based saccharides. Using a retaining mechanism, they can, in addition to hydrolysis, also potentially perform transglycosylation reactions, synthesizing new glyco-conjugates ...
Anna Rosengren, Derek T Logan, Henrik Stålbrand   +2 more
exaly   +2 more sources

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Enzymatic transglycosylation of xylose using a glycosynthase

Carbohydrate Research, 2005
The application of the hyperactive glycosynthase derived from Agrobacterium sp. beta-glucosidase (AbgE358G-2F6) to the synthesis of xylo-oligosaccharides by using alpha-D-xylopyranosyl fluoride as donor represents the first successful use of glycosynthase technology for xylosyl transfer.
Young-Wan, Kim, Hongming, Chen, Stephen G, Withers   +2 more
openaire   +2 more sources

Transglycosylation Reactions of 6-Thioguanine Acyclonucleosides

Nucleosides, Nucleotides and Nucleic Acids, 2000
9-(2-Acetoxyethoxy)methyl-N2-acetyl-6-thoguanine (2) undergoes two different transglycosylation reactions: i) the 79 isomerization, which gives the respective 7-regioisomer (3) as the major product, ii) the 9S6 glycosyl migration, which leads to a 9,S6-disubstituted product (4). S6-Methylation completely stopped the reversibility of transglycosylation.
A, Manikowski, J, Boryski
openaire   +2 more sources

Transglycosylation of L-ascorbic acid

Applied Biochemistry and Microbiology, 2007
Cyclodextrin glucanotransferases (CGTs, EC 2.4.1.19) from mesophilic, thermophilic, and halophilic bacteria and maltase (EC 3.2.1.20) from the yeast Saccharomyces cerevisiae were used for transglycosylation of ascorbic acid with starch, maltodextrin, γ-cyclodextrin, and maltose. These compounds served as donors of glucosyl residues.
A. A. Markosyan, L. A. Abelyan, M. O. Adamyan, Zh. I. Akopyan, V. A. Abelyan   +4 more
openaire   +3 more sources

Benzimidazoles in the Reaction of Enzymatic Transglycosylation

Nucleosides, Nucleotides and Nucleic Acids, 1995
Abstract Substrate activity of a broad spectrum of derivatives of benzimidazole in the reaction of enzymatic ribo- and 2-deoxyribosylation catalyzed by purine nucleoside phosphorylase of whole cells of E. coli BMT-1D/1A has been studied. Guanosine or 2′-deoxyguanosine were used as glycosyl donors.
Igor Mikhailopulo, Zygmunt Kazimierczuk, Anatoli Zinchenko, Vladimir Barai, Valeria Romanova, Ludmilla Eroshevskaya   +5 more
openaire   +1 more source

Enzymatic transglycosylation

2009
The most important functions of carbohydrates are structural roles and energy storage, but carbohydrate parts of glycoproteins and glycolipids are involved in biological functions mainly related to cell recognition events. Carbohydrates are also important molecules in the technological domain.
A Tramice, G Andreotti, A Giordano, A Trincone   +3 more
openaire   +1 more source

Oligosaccharide synthesis by enzymatic transglycosylation

Glycoconjugate Journal, 1990
Agricultural Research Service, United States Department of Agriculture, Northern Regional Research Center, 1815 N. University St., Peoria, IL 61604, U.S.A. 2 Biochemical and Food Process Engineering, Agricultural Engineering Department, Purdue University, West Lafayette, IN 47907, U.S.A.
Gregory L Cote, Bernard Y Tao
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[Transglycosylation of benzo[h]quinazolines].

Prikladnaia biokhimiia i mikrobiologiia, 2009
This was the first study to apply cyclodextrin glucanotransferases (CGTase, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli as well as pullulanase, beta-amylase, beta-galactosidase, and beta-fructofuranosidase for transglycosylation of benzo[h]quinazolines.
A A, Markosian, L A, Abelian, A I, Markosian, V A, Abelian   +3 more
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Transglycosylation Reaction of 6-Thioguanosine

Nucleosides and Nucleotides, 1999
Abstract Peracetylated 6-thioguanosine (2) undergoes the so far unknown N→S transglycosylation reaction to form a stable 9,S6 -bis(ribosyl) derivative (3) and N2-acetyl-6-thioguanine (4). The reaction takes place at elevated temperatures with no catalyst, or in solution in the presence of acidic catalysts.
Andrzej Manikowski, Jerzy Boryski
openaire   +1 more source

Transglycosylation in the Modification and Isotope Labeling of Pyrimidine Nucleosides

Organic Letters, 2020
Transglycosylation of pyrimidine nucleosides is demonstrated in a one-pot synthesis of uridine derivatives under microwave irradiation. Inductive activation of 2',3',5'-tri-O-acetyl uridine with a 5-nitro group produces a more-reactive glycosyl donor. Under optimized Vorbrüggen conditions, the 5-nitrouridine facilitates a reversible nucleobase exchange
Yong Gong, Lu Chen, Wei Zhang, Rhys Salter   +3 more
openaire   +2 more sources