Results 181 to 190 of about 8,066 (202)

Enzymatic transglycosylation

2009
The most important functions of carbohydrates are structural roles and energy storage, but carbohydrate parts of glycoproteins and glycolipids are involved in biological functions mainly related to cell recognition events. Carbohydrates are also important molecules in the technological domain.
A Tramice, G Andreotti, A Giordano, A Trincone   +3 more
openaire   +1 more source

β-Galactosidase-catalyzed intramolecular transglycosylation

Tetrahedron Letters, 2001
Abstract A novel transglycosylation strategy for efficient preparation of N -acetyllactosamine (Galβ1→4GlcNAc, LacNAc) and sialyl LacNAc was developed using β-galactosidase from Bacillus circulans . In order to minimize the competing hydrolysis by forcing the enzymatic transglycosylation to proceed in an intramolecular manner, a novel substrate 9
Shiro Komba, Yukishige Ito
openaire   +1 more source

[Transglycosylation of benzo[h]quinazolines].

Prikladnaia biokhimiia i mikrobiologiia, 2009
This was the first study to apply cyclodextrin glucanotransferases (CGTase, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli as well as pullulanase, beta-amylase, beta-galactosidase, and beta-fructofuranosidase for transglycosylation of benzo[h]quinazolines.
A A, Markosian, L A, Abelian, A I, Markosian, V A, Abelian   +3 more
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Transglycosylation to Ginseng Saponins by Cyclomaltodextrin Glucanotransferases

Bioscience, Biotechnology, and Biochemistry, 2001
Ten new alpha-glucosylginsenosides were found to be synthesized from dextrin and four ginsenosides, -Rb1, -Rc, -Re, and -Rg1, by the successive actions of B. stearothermophilus cyclomaltodextrin glucanotransferase and Rhizopus glucoamylase. Seven of them were isolated in the pure state by extraction with n-butanol saturated with water, silica gel ...
Y H, Kim, Y G, Lee, K J, Choi, K, Uchida, Y, Suzuki   +4 more
openaire   +2 more sources

Transglycosylation Activity of Endoglycoceramidase from Corynebacterium sp

Archives of Biochemistry and Biophysics, 1993
Endoglycoceramidase (EGCase) catalyzes the hydrolysis of the linkage between oligosaccharides and ceramides of various glycosphingolipids (GSLs). We found that the EGCase from Corynebacterium sp. had transglycosylation activity. Digesting GSLs with the enzyme in the presence of a suitable acceptor gave a mixture of hydrolytic and transglycosylic ...
H, Ashida, Y, Tsuji, K, Yamamoto, H, Kumagai, T, Tochikura   +4 more
openaire   +2 more sources

Enzymatic transglycosylation of PEG brushes by β-galactosidase

Chemical Communications, 2012
Enzymatic transglycosylation has been successfully implemented by β-galactosidase (β-Gal) on PEG brushes. Kinetic parameters validate this kind of chemoenzymatic strategy which is promising for construction of biomimetic surfaces with glycocalyx-like structures.
Yan, Fang, Wei, Xu, Jian, Wu, Zhi-Kang, Xu   +3 more
openaire   +2 more sources

Estimation of selectivities in transglycosylation systems with multiple transglycosylation products

Biochemical Engineering Journal, 2022
David Alexander Mitchell, Nadia Krieger
openaire   +1 more source

Transglycosylation of engineered cyclodextrin glucanotransferases as O-glycoligases

Carbohydrate Polymers, 2014
An O-glycoligase is a hydrolytically impaired mutant of a retaining α-glycosidase in which the catalytic acid/base has been removed, but which can still perform transglycosylation when incubated with activated glycosyl fluoride donor sugars. In this paper, we describe another example, wherein a cyclodextrin glucanotransferase mutant (CGT-E284A) with an
Chao, Li, Hee-Jeong, Ahn, Jin-Hyo, Kim, Young-Wan, Kim   +3 more
openaire   +2 more sources

Synthesis of Bioactive Glycopeptides Through Endoglycosidase‐Catalyzed Transglycosylation

ChemInform, 2004
AbstractFor Abstract see ChemInform Abstract in Full Text.
Lai-Xi Wang, Suddham Singh, Jiahong Ni
openaire   +1 more source

High-Yield Synthesis ofN-Acetyllactosamine by Regioselective Transglycosylation

Biochemical and Biophysical Research Communications, 1996
The synthesis of N-acetyllactosamine (D-Galp beta 1-4D-GlcpNAc) with very low contamination of its isomer N-acetyllactosamine (D-Galp beta 1-6D-GlcpNAc) was obtained by use of regioselective transglycosylation activity of beta-galactosidase from Bacillus circulans using lactose as the donor of D-Galp and D-GlcpNAc as the acceptor.
A. VETERE, PAOLETTI, SERGIO
openaire   +3 more sources