Results 41 to 50 of about 8,066 (202)

N-Ribosyltransferase From Archaeoglobus veneficus: A Novel Halotolerant and Thermostable Biocatalyst for the Synthesis of Purine Ribonucleoside Analogs

Frontiers in Bioengineering and Biotechnology, 2020
Nucleoside-2′-deoxyribosyl-transferases (NDTs) catalyze a transglycosylation reaction consisting of the exchange of the 2′-deoxyribose moiety between a purine and/or pyrimidine nucleoside and a purine and/or pyrimidine base.
Javier Acosta, Jon Del Arco, Victor Pisabarro, Federico Gago, Jesús Fernández-Lucas, Jesús Fernández-Lucas   +5 more
doaj   +1 more source

The versatile enzyme Araf51 allowed efficient synthesis of rare pathogen-related β-d-galactofuranosyl-pyranoside disaccharides. [PDF]

, 2014
International audienceThe preparation of galactofuranosyl-containing disaccharidic parts of natural glycoconjugates was performed according to a chemo-enzymatic synthesis.
Chlubnová, Ilona, Daniellou, Richard, Dvoráková, Hana, Ferrières, Vincent, Filipp, Dominik, Hošek, Petr, Králová, Blanka, Nugier-Chauvin, Caroline, Spiwok, Vojtech   +8 more
core   +6 more sources

Heterologous Expression of a Thermostable α-Glucosidase from Geobacillus sp. Strain HTA-462 by Escherichia coli and Its Potential Application for Isomaltose–Oligosaccharide Synthesis

Molecules, 2019
Isomaltose–oligosaccharides (IMOs), as food ingredients with prebiotic functionality, can be prepared via enzymatic synthesis using α-glucosidase. In the present study, the α-glucosidase (GSJ) from Geobacillus sp.
Fan Zhang, Weiyang Wang, Fatoumata Binta Maci Bah, Chengcheng Song, Yifa Zhou, Li Ji, Ye Yuan   +6 more
doaj   +1 more source

A Novel Dimeric Exoglucanase (GH5_38): Biochemical and Structural Characterisation towards its Application in Alkyl Cellobioside Synthesis

Molecules, 2020
An exoglucanase (Exg-D) from the glycoside hydrolase family 5 subfamily 38 (GH5_38) was heterologously expressed and structurally and biochemically characterised at a molecular level for its application in alkyl glycoside synthesis.
Mpho S. Mafa, Heinrich W. Dirr, Samkelo Malgas, Rui W. M. Krause, Konanani Rashamuse, Brett I. Pletschke   +5 more
doaj   +1 more source

Reassessment of Acarbose as a Transition State Analogue Inhibitor of Cyclodextrin Glycosyltransferase [PDF]

, 1998
The binding of several different active site mutants of Bacillus circulans cyclodextrin glycosyltransferase to the inhibitor acarbose has been investigated through measurement of Ki values.
Dijkstra, Bauke W.,, Mosi, Renee,, Ruiterkamp, Richard,, Sham, Howard,, Uitdehaag, Joost C.M.,, Withers, Stephen G.,   +5 more
core   +2 more sources

Intermolecular Transglycosylation of Cyclodextrin Glycosyltansferase

Journal of the Japanese Society of Starch Science, 1979
The acceptor specificity in the intermolecular transglycosylation of cyclodextrin glycosyltransferase (CGT-ase) was investigated using 18 kinds of monosaccharides and their derivatives. It is concluded on the basis of the experimental results that the requirement for an acceptor of the intermolecular transfer reaction catalyzed by CGT-ase is the ...
Sumio KITAHATA, Shigetaka OKADA
openaire   +2 more sources

Structural Enzymology of Cellvibrio japonicus Agd31B Protein Reveals α-Transglucosylase Activity in Glycoside Hydrolase Family 31 [PDF]

, 2012
The metabolism of the storage polysaccharides glycogen and starch is of vital importance to organisms from all domains of life. In bacteria, utilization of these -glucans requires the concerted action of a variety of enzymes, including glycoside ...
Adams, Atsushi Izumi, Ball, Barends, Becker, Berezina, Boos, Burlingham, Cantarel, Cowtan, Davies, Davies, DeBoy, Dippel, Elbein, Emsley, Ernst, Gasteiger, Gibson, Gideon J. Davies, Glyn R. Hemsworth, Harry Brumer, Hazlewood, Hendlich, Humphry, Janecek, Johan Larsbrink, Jones, Jung, Kato, Kim, Kim, Kim, Larsbrink, Lee, Lee, Leemhuis, Leslie, Liebl, Lovering, Meissner, Mukai, Okuyama, Palmer, Perrakis, Przylas, Ren, Roach, Rossi, Roujeinikova, Roujeinikova, Schwartz, Seo, Sim, Sim, Sinnott, Stam, Stick, Sundqvist, Suzuki, Takaha, Takaha, Tan, Terada, Vagin, Vonrhein, Wang, Wilson, Winn, Withers, Yamamoto, Zhou   +71 more
core   +1 more source

Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase

Molecules, 2023
The enzymatic transglycosylation of steviol glycosides can improve the edulcorant quality of steviol glycosides. Cyclodextrin glucanotransferase (CGTase) is one of the most popular glucanotransferases applied in this reaction.
Ruiqin Zhang, Ruiqi Tang, Jiahua Bi, Shanshan Shen, Qin Wu, Qihe Chen, Yanjun Li   +6 more
doaj   +1 more source

Active Site Mapping of Xylan-Deconstructing Enzymes with Arabinoxylan Oligosaccharides Produced by Automated Glycan Assembly [PDF]

, 2017
Xylan-degrading enzymes are crucial for the deconstruction of hemicellulosic biomass, making the hydrolysis products available for various industrial applications such as the production of biofuel.
de Kruijff, Goswinus H. M., Pfrengle, Fabian, Ruprecht, Colin, Schuhmacher, Frank, Seeberger, Peter H., Senf, Deborah, Simonetti, Sebastián Osvaldo   +6 more
core   +1 more source

Introducing transglycosylation activity in a liquefying α‐amylase [PDF]

FEBS Letters, 1999
By mutating Ala‐289 by Phe or Tyr in the Bacillus stearothermophilus α‐amylase, we induced this enzyme to perform alcoholytic reactions, a function not present in the wild‐type enzyme. This residue was selected from homology analysis with neopullulanase, where the residue has been implicated in the control of transglycosylation [Kuriki et al. (1996) J.
Saab-Rincón, Gloria, del-Rı́o, Gabriel, Santamarı́a, Rosa I, López-Munguı́a, Agustı́n, Soberón, Xavier   +4 more
openaire   +2 more sources