Results 51 to 60 of about 8,066 (202)

Transglycosylation reactions catalysed by two β-mannanases [PDF]

open access: yesBiochemical Journal, 1981
By using [3H]mannobiose as a labelled acceptor, it was possible to demonstrate transfer reactions catalysed by two beta-mannanases, with mannotetraose and mannopentaose as substrates. The enzyme from Streptomyces transfers one mannose unit from the oligosaccharides, whereas the enzyme from fenugreek (Trigonella foenum-graecum) seeds is able to transfer
C, Coulombel   +3 more
openaire   +2 more sources

Sequence‐Based and Functional Analysis for the Discovery of N‐Glycan Degrading Glycosidases From the Microbial Metagenome of the Infant Gut

open access: yesMicrobiologyOpen, Volume 15, Issue 2, April 2026.
This research offers valuable understanding of how N‐glycans are broken down by the gut microbiota of infants, focusing on glycoside hydrolase families GH2, GH20, and GH18. It reveals that the gut microbiota of breastfed infants has a diverse array of genes coding for these enzymes.
Irene Boscá‐Sánchez   +2 more
wiley   +1 more source

Non-Proteinogenic Amino Acids in Lacticin 481 Analogues Result in More Potent Inhibition of Peptidoglycan Transglycosylation [PDF]

open access: yes, 2012
Lantibiotics are ribosomally synthesized and post-translationally modified peptide natural products that contain the thioether structures lanthionine and methyllanthionine and exert potent antimicrobial activity against Gram-positive bacteria. At present,
Garcia De Gonzalo, Chantal V.   +5 more
core   +1 more source

Antimicrobial Effect of Spices and Their Phytochemicals: A Novel Approach to Overcoming Antibiotic Resistance

open access: yesMedComm, Volume 7, Issue 4, April 2026.
This graphical abstract shows the antibacterial potential of phytochemicals from 15 spices, showcasing key compounds, their extraction methods, and mechanisms of action. It highlights membrane disruption, ROS generation, enzyme inhibition, and biofilm prevention as core antibacterial pathways.
Hettiyahandi Binodh De Silva   +3 more
wiley   +1 more source

Transglycosylation products generated by Talaromyces amestolkiae GH3 β-glucosidases: effect of hydroxytyrosol, vanillin and its glucosides on breast cancer cells

open access: yesMicrobial Cell Factories, 2019
Background Transglycosylation represents one of the most promising approaches for obtaining novel glycosides, and plant phenols and polyphenols are emerging as one of the best targets for creating new molecules with enhanced capacities.
Juan Antonio Méndez-Líter   +10 more
doaj   +1 more source

Computer‐Based Design to Improve Bacillus thuringiensis Chitinase for Industrial Applications

open access: yesChemistryOpen, Volume 15, Issue 4, April 2026.
Hotspot‐guided mutagenesis identifies Gly291 as a key catalytic determinant in Bacillus thuringiensis chitinase. Substitution with alanine improves substrate accommodation and lowers the activation barrier by ∼47%, reducing it from 94.66 to 49.83 kcal/mol. Structural integrity is retained while catalytic efficiency is enhanced, demonstrating a rational
S. G. Sree Agash   +5 more
wiley   +1 more source

Effects of monovalent cations (Na+ and K+) on galacto-oligosaccharides production during lactose hydrolysis by kluyveromyces lactis β-galactosidase [PDF]

open access: yes, 2014
The influence of cations, Na+ and K+ on the oligosaccharides produced in the process of transglycosylation during the hydrolysis of lactose using β-galactosidase from Kluyveromyces (K.) lactis has been investigated. The enzyme exhibited higher hydrolytic
Corzo, Nieves   +2 more
core   +1 more source

Synthesis of Human Milk Oligosaccharides: Protein Engineering Strategies for Improved Enzymatic Transglycosylation

open access: yesMolecules, 2019
Human milk oligosaccharides (HMOs) signify a unique group of oligosaccharides in breast milk, which is of major importance for infant health and development.
Birgitte Zeuner   +3 more
doaj   +1 more source

Transglycosylating β‐d‐galactosidase and α‐l‐fucosidase from Paenibacillus sp. 3179 from a hot spring in East Greenland

open access: yesMicrobiologyOpen, 2020
Thermal springs are excellent locations for discovery of thermostable microorganisms and enzymes. In this study, we identify a novel thermotolerant bacterial strain related to Paenibacillus dendritiformis, denoted Paenibacillus sp.
Mariane S. Thøgersen   +4 more
doaj   +1 more source

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