Results 61 to 70 of about 8,066 (202)

Alteration of Substrate Specificity and Transglucosylation Activity of GH13_31 α-Glucosidase from Bacillus sp. AHU2216 through Site-Directed Mutagenesis of Asn258 on β→α Loop 5

open access: yesMolecules, 2023
α-Glucosidase catalyzes the hydrolysis of α-d-glucosides and transglucosylation. Bacillus sp. AHU2216 α-glucosidase (BspAG13_31A), belonging to the glycoside hydrolase family 13 subfamily 31, specifically cleaves α-(1→4)-glucosidic linkages and shows ...
Waraporn Auiewiriyanukul   +6 more
doaj   +1 more source

The Formation of Covalent Linkages in Lignocellulosic Biomass via the Oxocarbenium Intermediate

open access: yesChemPhysChem, Volume 27, Issue 4, 25 February 2026.
Density functional theory models show that the hydrated and acidic hemicellulose matrix in lignocellulosic biomass can generate oxocarbenium ions. These reactive intermediates can participate in the stable formation of glycosidic lignin‐carbohydrate covalent linkages, promoting recalcitrance in plant biomass.
Eduardo Romero‐Montalvo   +1 more
wiley   +1 more source

The mucin-degradation strategy of Ruminococcus gnavus:The importance of intramolecular trans-sialidases [PDF]

open access: yes, 2016
We previously identified and characterized an intramolecular trans-sialidase (IT-sialidase) in the gut symbiont Ruminococcus gnavus ATCC 29149, which is associated to the ability of the strain to grow on mucins. In this work we have obtained and analyzed
Crossman, Lisa C.   +6 more
core   +2 more sources

Characterization of a Novel Ginsenoside MT1 Produced by an Enzymatic Transrhamnosylation of Protopanaxatriol-Type Ginsenosides Re

open access: yesBiomolecules, 2020
Background: Ginsenosides, triterpene saponins of Panax species, are considered the main active ingredients responsible for various pharmacological activities.
Byeong-Min Jeon   +4 more
doaj   +1 more source

Transglycosylation Activity of Glycosynthase Mutants of Endo-β-N-Acetylglucosaminidase from Coprinopsis cinerea. [PDF]

open access: yesPLoS ONE, 2015
Endo-β-N-acetylglucosaminidase (ENGase), which catalyzes hydrolysis of N-linked oligosaccharides, is a useful tool for analyzing oligosaccharide contents of glycoproteins.
Yasunari Eshima   +4 more
doaj   +1 more source

Biochemical characterization of a novel β-galactosidase from Lacticaseibacillus zeae and its application in synthesis of lacto-N-tetraose

open access: yesJournal of Dairy Science, 2023
: Lacto-N-tetraose (LNT) is one of the most important components of human milk oligosaccharides, which has various beneficial health effects. β-Galactosidase is an important enzyme used in dairy processing.
Ting Li   +4 more
doaj   +1 more source

Microbial β-Glucosidases: screening, characterization, cloning and applications [PDF]

open access: yes, 2017
Cellulose is the most abundant biomaterial in the biosphere and the major component of plant biomass. Cellulase is an enzymatic system required for conversion of renewable cellulose biomass into free sugar for subsequent use in different applications ...
Ahmed, Amer   +5 more
core  

Channel-forming activity of syringopeptin 25 A in mercury-supported phospholipid monolayers and negatively charged bilayers [PDF]

open access: yes, 2016
Interactions of the cationic lipodepsipeptide syringopeptin 25 A (SP25A) with mercury-supported dioleoylphosphatidylcholine (DOPC), dioleoylphosphatidylserine (DOPS) and dioeleoylphosphatidic acid (DOPA) self-assembled monolayers (SAMs) were investigated
Becucci, Lucia   +4 more
core   +2 more sources

Screening Systems for Detecting Inhibitors of Cell Wall Transglycosylation in Enterococcus.Cell Wall Transglycosylation Inhibitors in Enterococcus.

open access: yesThe Journal of Antibiotics, 1998
We devised two screening systems to detect cell wall transglycosylation inhibitors. One screen utilizes a mutant of Enterococcus faecalis strain A256 that is dependent on vancomycin or moenomycin for growth. In the absence of transglycosylation inhibitors the strain fails to grow, while in the presence of inhibitors, cells are rescued.
N, Mani   +10 more
openaire   +3 more sources

Transglycosylation: A mechanism for RNA modification (and editing?) [PDF]

open access: yesBioorganic Chemistry, 2005
The vast majority of the ca. 100 chemically distinct modified nucleosides in RNA appear to arise via the chemical transformation of a genetically encoded nucleoside. Two notable exceptions are queuosine and pseudouridine, which are incorporated into tRNA via transglycosylation.
George A, Garcia, Jeffrey D, Kittendorf
openaire   +2 more sources

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