Plant chloroplasts enable high-yield and cost-effective production of the engineered bacterial enzyme CueO-MacHis as a technical protein via transient expression in <i>Nicotiana benthamiana</i>. [PDF]
Nausch H +3 more
europepmc +1 more source
USP7 inhibits FOXO1 activity in chronic lymphocytic leukaemia cells enhancing cell survival
CLL cells rely on BCR‐mediated signalling for survival and proliferation in part through suppression of FOXO1. FOXO1 activity is regulated by a plethora of post‐translational modifications including phosphorylation and ubiquitination. We demonstrated that dual inhibition of BCR signalling and the deubiquitinase USP7 activates FOXO1, modulating its ...
Hassan N.B. Almuhanna +8 more
wiley +1 more source
Transient Expression and Immunogenicity Assessment of the <i>Dermatophagoides pteronyssinus</i> Der p 2 Allergen Produced in <i>Nicotiana benthamiana</i>. [PDF]
Jirananon K +4 more
europepmc +1 more source
Production and stably maintenance of strigolactone by transient expression of biosynthetic enzymes in Nicotiana benthamiana. [PDF]
Yata A +4 more
europepmc +1 more source
The impact of leukocyte‐associated RNA retention on platelet RNA‐based cancer detection
Platelets carry RNA signals that may help detect cancer, but platelet samples can also retain RNA from white blood cells. We found that this retained white blood cell signal did not significantly affect machine learning cancer classification, suggesting that the cancer‐related RNA signal in platelets is largely independent of leukocyte‐associated RNA ...
Michał Sieczczyński +12 more
wiley +1 more source
Comparing hormone dynamics in cereal crops via transient expression of hormone sensors. [PDF]
Dao TQ, Drapek C, Jones A, Leiboff S.
europepmc +1 more source
Protoplast-Based Transient Expression and Gene Editing in Shrub Willow (Salix purpurea L.). [PDF]
Hyden B +5 more
europepmc +1 more source
Vascular normalization confers an endothelial immune suppressive gene expression profile
We compare the immunoregulatory gene expression profiles of healthy endothelial cells or endothelial cells from tumors having a normalized endothelium (low leakage) with that of wild‐type tumors (high leakage) and observe that the wild‐type tumor endothelium exhibits immunostimulatory properties.
Liqun He, Michael Welsh
wiley +1 more source
The Impacts of Environmental Factors and Culture Conditions on the Transient Expression of Recombinant Protein in Nicotiana benthamiana Under Controlled Environments. [PDF]
Matsuda R.
europepmc +1 more source
Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera. [PDF]
Li S +8 more
europepmc +1 more source

