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Midkine expression in transient retinal ischemia in the rat
Current Eye Research, 1998Midkine (MK), a 13-kDa heparin-binding growth factor, is known to exert neurotrophic activities on various nerve cells including retinal cells. To initiate studies toward determining the physiological role of endogenous MK, we investigated the spatial and temporal expression profile of MK before and after intraocular pressure-induced retinal ischemia ...
M, Miyashiro +7 more
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Transient Gene Expression in Human Expi293 Cells
2022Transient gene expression (TGE) is an important tool for generating recombinant proteins in a short period of time. The human cell line HEK293 is widely used for this purpose since it can grow in suspension to a high cell density in serum-free media.
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Improved Transient Protein Expression by pFluNS1 Plasmid
Molecular Biotechnology, 2013Influenza virus nonstructural protein-1 (NS1) is abundantly expressed in influenza virus infected cells. NS1 is well recognized for counteracting host antiviral activities and regulating host and viral protein expression. When used as a plasmid component in DNA transfection, NS1 was shown to significantly increase expression levels of a cotransfected ...
Jarin, Kramyu +3 more
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Variation in enzymatic transient gene expression assays
Analytical Biochemistry, 1989We examined causes for high variability in data from enzymatic transient gene expression assays. Our results strongly suggest that variation in transfection efficiency is the major cause of data variation and can seriously compromise valid interpretation of data.
T, Hollon, F K, Yoshimura
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Transient Expression of GFP in Immature Seed Tissues
2009Transient expression is the nonstable expression of a transgene whereby the transgene does not integrate into the host's genomic DNA. Transient expression assays have 20 years of history in plant molecular biology research, being used to answer a variety of questions.
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Transient and Stable Expression of Antibodies in Nicotiana Species
2012Expression and purification of recombinant proteins produced in plants is emerging as an affordable alternative to using more costly mammalian bioreactors since plants are capable of producing mammalian proteins at high concentrations. There are two general methods of expressing foreign proteins in plants, namely, transient expression and stable ...
Freydoun, Garabagi +2 more
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Coupled enzyme assays: A general expression for the transient
Biochimica et Biophysica Acta (BBA) - Enzymology, 1973Abstract The rate of formation of product in a coupled enzyme reaction is subject to a transient phase prior to the attainment of the steady-state. General expressions are presented to describe the transient in a multi-enzyme sequence in which the initial enzyme is rate-limiting.
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A simple and efficient agroinfiltration method for transient gene expression in Citrus
Plant Cell Reports, 2021Stacy Welker +2 more
exaly
Transient Gene Transfer and Expression in the Lung
Chest, 1997P J, Sime +4 more
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