Results 251 to 260 of about 270,633 (290)

Transient transfection of Theileria annulata

Molecular and Biochemical Parasitology, 2001
We have developed a method to transiently transfect infective, uninucleate, Theileria annulata sporozoites. Transfection vectors have been constructed using a number of T. annulata 5' gene flanking sequences linked to the enhanced green fluorescence protein (eGFP) reporter gene.
R, Adamson, K, Lyons, M, Sharrard, J, Kinnaird, D, Swan, S, Graham, B, Shiels, R, Hall   +7 more
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Retroviral Vector Production by Transient Transfection

Cold Spring Harbor Protocols, 2008
INTRODUCTIONThis protocol describes vector production by transient transfection. The production of retroviral vectors requires a full-length copy of the vector RNA to be incorporated into virions. This is accomplished by coexpressing vector RNA and the viral proteins required for virion formation from expression plasmids.
Kenneth, Cornetta, Karen E, Pollok, A Dusty, Miller   +2 more
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Transient transfection of DT40

2006
Transient transfection of cell lines can be extremely useful in a number of applications. However, until recently, obtaining useful and reproducible percentages of transiently expressing DT40 was almost impossible. Standard techniques such as electroporation usually give low transfection efficiencies (can be up to 40%, but ususally less than 5%) while ...
Roger, Franklin, Julian E, Sale
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Transient Transfection of Oligodendrocyte Progenitors by Electroporation

Neurochemical Research, 1998
The transient transfection of transgenes into oligodendrocytes offers an important tool for studying the function of proteins during myelin formation. Currently established procedures, however, have generally resulted in low survival rates and low levels of uptake of the transgene into primary oligodendrocyte progenitors. We describe an electroporation
W H, Krueger, D L, Madison, S E, Pfeiffer   +2 more
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Keratinocyte Transient Transfections

2004
The goal of this chapter is to give the reader a concise and easy-to-follow guide to proven transient transfection techniques for primary strains and continuous lines of keratinocytes. The emphasis is on readily available and inexpensive resources that also allow for repeatability and adaptability to scale, experimental conditions, and sample ...
Anthony M, Flores, Brian J, Aneskievich
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Transient transfection of purified Babesia bovis merozoites

Experimental Parasitology, 2008
Transient transfection of intraerythrocytic Babesia bovis parasites has been previously reported. In this study, we describe the development and optimization of methods for transfection of purified B. bovis merozoites using either nucleofection (Amaxa) or conventional electroporation (Gene Pulser II, BioRad).
Carlos E, Suarez, Terry F, McElwain
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In trans promoter activation by enhancers in transient transfection

Gene, 2017
Earlier, it was reported that the strong cytomegalovirus enhancer can activate the cytomegalovirus promoter in trans, i.e. as a separate plasmid co-transfected with a promoter-reporter gene construct. Here we demonstrate that the ability of enhancers to activate promoters in trans in transient transfection experiments is a property of not only viral ...
N A, Smirnov, S B, Akopov, D A, Didych, L G, Nikolaev   +3 more
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Survival assay of transiently transfected dopaminergic neurons

Journal of Neuroscience Methods, 2008
Death pathways in the apoptotic neurons are mostly studied by manipulating the levels of apoptosis-related proteins and counting the survival/death of affected neurons. Such assays are, however, technically complicated. We developed a transfection-survival assay for cultured embryonic dopaminergic (DA) neurons induced to die by deprivation of glial ...
Li-Ying, Yu, Urmas, Arumäe
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Transient transfection of human peripheral blood basophils

Journal of Immunological Methods, 2005
The human basophil has resisted previous attempts at transient transfection. Basophils were transfected by nucleoporation and to test whether there was sufficient expression to modify cell function, the cells were transfected with a syk kinase tandem SH2 construct linked to GFP.
Natalia, Vilariño, Donald, MacGlashan
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Transient Transfection in Mammalian Cells

2006
The aim of the presented work was to develop a cost-effective and easily scaleable transient transfection system with mammalian cells grown in serum-free suspension culture. For this purpose the cationic polyethylenimine (PEI) and the novel hybrid molecule dioleoyl-melittin (DOM) were used. Both substances are highly efficient transfection reagents for
E. -J. Schlaeger, J. Y. Legendre, A. Trzeciak, E. A. Kitas, K. Christensen, U. Deuschle, A. Supersaxo   +6 more
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