Results 261 to 270 of about 270,633 (290)
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Transient and stable transfection of Leishmania by particle bombardment
Molecular and Biochemical Parasitology, 1998Transfection of trypanosomatids, particularly the transfection of their mitochondrion and different developmental stages, is still a limiting step in many experiments using recombinant DNA technology. Two types of trypanosomatid transfection protocols have been described so far.
Sbicego S, Schnaufer A, Blum B
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Transient transfection of chick-embryo hepatocytes
The Journal of Nutritional Biochemistry, 1993Abstract Nutritional state regulates the expression of many genes via alterations in the plasma levels of hormones or metabolic fuels. In many cases, transcription has been identified as the regulated step. The next objective in the experimental analysis of these transcriptionally regulated genes is to identify the cis -acting sequence elements that
Rebecca A. Baillie +2 more
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100 Liter Transient Transfection
2001Reference LBTC-CONF-2001-001View record in Web of Science Record created on 2007-07-20, modified on 2016-08 ...
Philippe Girard +5 more
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Transient Transfection Assay of Cell Death Genes
2000In conclusion, transient transfection is an efficient and powerful method to determine quickly whether a gene has a detrimental effect on cell survival. We have described a variety of assay systems from which to choose. Each system has its own advantages and disadvantages.
M, Miura, J, Yuan
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Brugia malayi: transient transfection by microinjection and particle bombardment
Experimental Parasitology, 2002To develop a method for the introduction of DNA into filarial parasites, several methods that have proven successful in other organisms were evaluated for their ability to transform Brugia malayi. Luciferase activity was detectable in embryos bombarded with gold particles coated with a construct consisting of a luciferase reporter gene under the ...
Tarig B, Higazi +4 more
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Transient Transfection of Plasmodium vivax Blood-Stage Parasites
2012Here, we describe the methodology for transient transfection of Plasmodium vivax. The ability to genetically manipulate P. vivax has rendered this important human malaria parasite more amenable to molecular investigation. However, a systematic analysis of this parasite and its disease-mediating interactions with the human host still awaits further ...
Cecilia P, Sanchez +3 more
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A new reporter gene for transient transfection of Plasmodium falciparum
Parasitology Research, 2003Transfection of Plasmodium falciparum has remained difficult and laborious due to a lack of suitable reporter genes and low transfection efficiency. Therefore, the luciferase gene of Renilla reniformis, a sensitive mammalian reporter gene, was evaluated as a reporter gene in this system. Our studies indicate that the R.
Kevin T, Militello, Dyann F, Wirth
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Transient transfection of HeLa cells for analysis of splicing reaction
1990Publisher Summary This chapter describes methods that analyze the transient transfection of HeLa cells and the isolation of the RNA from these cells. Mutations are introduced in the large intron of the rabbit β-globin gene, and the resulting constructs are used for the transient transformation of HeLa cells.
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Mammalian Transient Transfection System
Genetic Engineering & Biotechnology News, 2012Meredith B. Jones +5 more
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