Results 91 to 100 of about 361 (118)
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Studying the Translatome With Polysome Profiling
Methods in Molecular Biology, 2016Polysome fractionation by sucrose density gradient centrifugation followed by analysis of RNA and protein is a technique that allows to understand the changes in translation of individual mRNAs as well as genome-wide effects on the translatome. Here, we describe the polysome profiling technique and RNA as well as protein isolation procedures from ...
Angelika Modelska, Paola Zuccotti
exaly +3 more sources
A hidden translatome in tumors—the coding lncRNAs
Science China Life Sciences, 2023Long noncoding RNAs (lncRNAs) have been extensively identified in eukaryotic genomes and have been shown to play critical roles in the development of multiple cancers. Through the application and development of ribosome analysis and sequencing technologies, advanced studies have discovered the translation of lncRNAs.
Jinsong, Wang +3 more
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Data on Translatome Analysis of Mycoplasma gallisepticum
Data in Brief, 2016 Mycoplasma gallisepticum is a bacterium of class Mollicutes which encompasses wall-less bacteria with significantly reduced genomes. Due to their overall reduction and simplicity mycoplasmas serve as a model of minimal cell and are used for systems biology studies.
Daria V Evsyutina, V M Govorun
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Affinity Purification of Ribosomes to Access the Translatome
Methods, 2009We describe ribosome affinity purification (RAP), a method that allows rapid purification of ribosomes and associated messages from the yeast Saccharomyces cerevisiae. The method relies on the expression of protein A tagged versions of the ribosomal protein Rpl16, which is used to efficiently recover endogenously formed ribosomes and polysomes from ...
André P. Gerber, Tanja Scherrer
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Regulation of the Translatome in Cardiac Remodeling
The FASEB Journal, 2022Gene expression is determined by both transcriptional as well as multiple layers of post‐transcriptional controls, including protein translation. One of the molecular hallmark observed in diseased hearts is augmented protein synthesis and marked reprogramming of translatome (defined as ribosomal loaded mRNAs).
Yibin Wang +2 more
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Translational control of immune responses: from transcripts to translatomes
Nature Immunology, 2014Selective translational control of gene expression is emerging as a principal mechanism for the regulation of protein abundance that determines a variety of functions in both the adaptive immune system and the innate immune system. The translation-initiation factor eIF4E acts as a node for such regulation, but non-eIF4E mechanisms are also prevalent ...
Piccirillo, Ciriaco A. +4 more
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Sex Differences in Developmental Patterns of Neocortical Astroglia: A Mouse Translatome Database
Cell Reports, 2022Stephanie Simard +2 more
exaly