Results 61 to 70 of about 5,654 (195)
Translation control can shape TP53-dependent cell fate
Molecular & Cellular Oncology, 2020 The search for mechanisms underlying different cellular responses to the treatment with Nutlin-3, an MDM2 inhibitor that unleashes p53, revealed a translational control mechanism involving the RNA binding proteins PCBP2 and, particularly, DHX30.
Dario Rizzotto +4 more
doaj +1 more source
Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion [PDF]
, 2017 Recombinant protein production coopts the host cell machinery to provide high protein yields of industrial enzymes or biotherapeutics. However, since protein translation is energetically expensive and tightly controlled, it is unclear if highly expressed
Andersen, Mikael Rørdam +8 more
core +3 more sources
A spatiotemporal translatome of mouse tissue development [PDF]
, 2020 AbstractThe precise regulation of gene expression in mammalian tissues during development results in their functional specification. Although previous transcriptomic and proteomic analyses have provided great biological insights into tissue-specific gene expression and the physiological relevance of these tissues in development, our understanding of ...
Wang, Hongwei +6 more
openaire +1 more source
Unbiased translation proteomics upon cell stress
Molecular & Cellular Oncology, 2020 The mammalian target of rapamycin and the integrated stress response are central cellular hubs regulating translation upon stress. The precise proteins and pathway specificity of translation targets of these pathways remained largely unclear. We recently
Kevin Klann, Christian Münch
doaj +1 more source
Generation and characterization of a mouse line for monitoring translation in dopaminergic neurons [PDF]
, 2017 We developed a mouse line targeting midbrain dopamine neurons for Translating Ribosome Affinity Purification(TRAP). Here, we briefly report on the basic characterization of this mouse line including confirmation of expression of the transgene in midbrain
Dougherty, Joseph D
core +2 more sources
Predicting the Impact of Alternative Splicing on Plant MADS Domain Protein Function [PDF]
Several genome-wide studies demonstrated that alternative splicing (AS) significantly increases the transcriptome complexity in plants. However, the impact of AS on the functional diversity of proteins is difficult to assess using genome-wide approaches.
Busscher-Lange, J. +5 more
core +2 more sources
What Is the Impact of mRNA 5′ TL Heterogeneity on Translational Start Site Selection and the Mammalian Cellular Phenotype? [PDF]
, 2016 A major determinant in the efficiency of ribosome loading onto mRNAs is the 5′ TL (transcript leader or 5′ UTR). In addition, elements within this region also impact on start site selection demonstrating that it can modulate the protein readout at both ...
Benjamin Weiss, Joseph A. Curran
core +2 more sources
Identification of neurodegenerative factors using translatome–regulatory network analysis [PDF]
Nature Neuroscience, 2015 For degenerative disorders of the CNS, the main obstacle to therapeutic advancement has been the challenge of identifying the key molecular mechanisms underlying neuronal loss. We developed a combinatorial approach including translational profiling and brain regulatory network analysis to search for key determinants of neuronal survival or death ...
Lars Brichta +11 more
openaire +2 more sources
Recent advances in malaria genomics and epigenomics [PDF]
, 2016 Malaria continues to impose a significant disease burden on low- and middle-income countries in the tropics. However, revolutionary progress over the last 3 years in nucleic acid sequencing, reverse genetics, and post-genome analyses has generated step ...
Kirchner, Sebastian +2 more
core +1 more source
Nature Communications, 2022 Alzheimer’s disease is associated with changes in astrocytes. Here the authors investigated the astrocyte translatome associated with amyloid-ß and tau pathology.
Zoeb Jiwaji +30 more
doaj +1 more source