Results 61 to 70 of about 3,685 (305)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Kompetencje zawodowe tłumacza audiowizualnego – między teorią a praktyką

Między Oryginałem a Przekładem, 2020
Professional Competences of an Audiovisual Translator – between Theory and Practice The article aims to outline the subject of specialized translation, including audiovisual translation (AV).
Magdalena Szpilman, Aleksandra Hasior
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

An evaluation of the EMT: Compatibility with the professional translator’s needs

Cogent Arts & Humanities, 2019
Appeal towards training translators as a sector in translation studies has had a dramatic increase throughout the past decade due to the needs for intercultural communication.
Mohammad Reza Esfandiari, Nasrin Shokrpour, Forough Rahimi   +2 more
doaj   +1 more source

The online computer-assisted translation classroom

Translation and Interpreting : the International Journal of Translation and Interpreting Research, 2020
In the modern translation industry, all actors involved in the translation process communicate and collaborate increasingly – and oftentimes entirely – online using modern computer-assisted translation and project management software solutions.New ...
Viktorija Bilic
doaj   +1 more source

Prospective translators' responsibilities in the transition from translation competence to translator competence and contributions of translation education in this process

, 2021
This study focuses on the concept of translation competence, which develops further with professionalization following the translation education and evolves into the concept of translator competence in practice. For this purpose, the word “competence” was first defined from different perspectives and the synonyms of the term were explained. After that,
Odacıoğlu, Mehmet Cem, Odacıoğlu, Mehmet Cem   +1 more
openaire   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

FREE OPEN SOURCE AND SOFTWARE IN THE TEACHING OF CAT TOOLS: OMEGAT, A VIABLE ALTERNATIVE

Texto Livre: Linguagem e Tecnologia, 2016
The teaching of Computer Aided Translation (CAT) tools is essential to all courses aimed at preparing students for the effective exercise of this profession, in particular those students who will translate technical texts, especially in the field of ...
Adauto Lúcio Caetano Villela
doaj   +1 more source