Results 171 to 180 of about 13,603 (194)
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Journal of Virological Methods, 2016
This research aimed to develop an oral vaccine for transmissible gastroenteritis virus (TGEV) based on the TGEV N gene expressed in an attenuated Salmonella typhimurium vector and aimed to evaluate the vaccine's immune response in piglets. Recombinant plasmid pVAX-N was transformed into competent cells of attenuated S.
Dan, Zhang +7 more
openaire +4 more sources
This research aimed to develop an oral vaccine for transmissible gastroenteritis virus (TGEV) based on the TGEV N gene expressed in an attenuated Salmonella typhimurium vector and aimed to evaluate the vaccine's immune response in piglets. Recombinant plasmid pVAX-N was transformed into competent cells of attenuated S.
Dan, Zhang +7 more
openaire +4 more sources
Veterinary Microbiology, 2021
Our previous research obtained purified recombinant porcine interferon-α (rPoIFN-α) containing thioredoxin (Trx) fusion tag in E. coli Rosetta (DE3). Here, we evaluate the efficacy of this rPoIFN-α to prevent piglets from the infection of the transmissible gastroenteritis virus (TGEV) attack.
Dong-Mei Gao +6 more
openaire +2 more sources
Our previous research obtained purified recombinant porcine interferon-α (rPoIFN-α) containing thioredoxin (Trx) fusion tag in E. coli Rosetta (DE3). Here, we evaluate the efficacy of this rPoIFN-α to prevent piglets from the infection of the transmissible gastroenteritis virus (TGEV) attack.
Dong-Mei Gao +6 more
openaire +2 more sources
Characterization of the Transmissible Gastroenteritis Virus (TGEV) Transcription Initiation Sequence
1995The ability of the TGEV transcription initiation sequence (TIS) to produce subgenomic RNAs was investigated by placing a reporter gene, chloramphenicol acetyltransferase (CAT) under the control of either the mRNA 6 or the mRNA 7 TISs. Both constructs only produced CAT in TGEV infected cells and the amount of CAT produced from the mRNA 7 TIS was less ...
J A, Hiscox +3 more
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American Journal of Veterinary Research, 1997
Abstract Objective Baculovirus-expressed transmissible gastroenteritis virus (TGEV) spike (S) glycoprotein vaccines were inoculated parenterally in swine to determine whether such vaccines could induce serum and whey virus-neutralizing (VN) antibodies and protective lactogenic immunity for TGEV-challenge-exposed pigs.
D I, Shoup, D J, Jackwood, L J, Saif
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Abstract Objective Baculovirus-expressed transmissible gastroenteritis virus (TGEV) spike (S) glycoprotein vaccines were inoculated parenterally in swine to determine whether such vaccines could induce serum and whey virus-neutralizing (VN) antibodies and protective lactogenic immunity for TGEV-challenge-exposed pigs.
D I, Shoup, D J, Jackwood, L J, Saif
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American Journal of Veterinary Research, 1992
Summary Five nonneutralizing monoclonal antibodies (MAb) generated to the virulent Miller strain of transmissible gastroenteritis virus (tgev) and specific for the S protein were characterized. Competition assays between purified and biotinylated MAb indicated that MAb 75B10 and 8G11 mapped near a new subsite, designated V and 2 MAb, 44C11 and 45A8 ...
R A, Simkins +3 more
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Summary Five nonneutralizing monoclonal antibodies (MAb) generated to the virulent Miller strain of transmissible gastroenteritis virus (tgev) and specific for the S protein were characterized. Competition assays between purified and biotinylated MAb indicated that MAb 75B10 and 8G11 mapped near a new subsite, designated V and 2 MAb, 44C11 and 45A8 ...
R A, Simkins +3 more
openaire +2 more sources
Antibody Response in Swine to Individual Transmissible Gastroenteritis Virus (TGEV) Proteins
1987Transmissible gastroenteritis virus (TGEV) is a coronavirus that produces a life-threatening diarrhea in neonatal pigs. Whereas sows infected with virulent virus produce protective colostral antibody, sows vaccinated with attenuated virus are significantly less effective in passively protecting baby pigs.
R, Wesley, R, Woods, P, Kapke
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The Genome of Transmissible Gastroenteritis Virus (TGEV)
1984Lysis of isolated TGE virions with sodium dodecyl sulphate (SDS) at room temperature followed by electrophoresis, yields predominantly a single band of RNA running at about molecular weight 6.8 × 106. Heating the SDS lysate at 80°C for 15 min prior to electrophoresis results in a cluster of bands of between 0.2–6.8 × 106.
Lynne Bountiff +3 more
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American Journal of Veterinary Research, 1998
Abstract Objective To compare recombinant transmissible gastroenteritis virus (TGEV) spike protein, (SP) R2-2, with attenuated live virus (ALV) vaccine in sows during late pregnancy. Animals 13 TGEV-seronegative sows and their pigs. Procedure At prepartum weeks (PPW) 6 and 4, sows of groups 1 and 2 received ALV via the oral/intranasal (O/IN) route.
S, Park +6 more
openaire +2 more sources
Abstract Objective To compare recombinant transmissible gastroenteritis virus (TGEV) spike protein, (SP) R2-2, with attenuated live virus (ALV) vaccine in sows during late pregnancy. Animals 13 TGEV-seronegative sows and their pigs. Procedure At prepartum weeks (PPW) 6 and 4, sows of groups 1 and 2 received ALV via the oral/intranasal (O/IN) route.
S, Park +6 more
openaire +2 more sources
American Journal of Veterinary Research, 1993
SUMMARY Monoclonal antibodies (MAB) to subsite A (25C9) and subsite D (44C11) of the S protein of transmissible gastroenteritis virus (tgev) were used in a blocking elisa on fixed tgev-infected swine testis cells to differentiate sera from pigs experimentally inoculated with either tgev or porcine respiratory coronavirus (prcv).
R A, Simkins +4 more
openaire +2 more sources
SUMMARY Monoclonal antibodies (MAB) to subsite A (25C9) and subsite D (44C11) of the S protein of transmissible gastroenteritis virus (tgev) were used in a blocking elisa on fixed tgev-infected swine testis cells to differentiate sera from pigs experimentally inoculated with either tgev or porcine respiratory coronavirus (prcv).
R A, Simkins +4 more
openaire +2 more sources
Monoclonal Antibodies in Immunodiagnosis & Immunotherapy, 2013
Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the ...
Qiong, Zhao +9 more
openaire +2 more sources
Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the ...
Qiong, Zhao +9 more
openaire +2 more sources