Results 171 to 180 of about 1,172,761 (182)
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2011 4th International Conference on Biomedical Engineering and Informatics (BMEI), 2011
This study established a SYBR Green I real-time quantitative PCR detection assay (RT-qPCR) for the detection of the porcine transmissible gastroenteritis virus (TGEV) using specific primers designed to amplify the highly conserved porcine TGEV S gene sequence. The threshold cycle (Ct) and the log plasmid copy numbers had a good linear relationship with
Li Cui, Congli Yuan, Xiuguo Hua
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This study established a SYBR Green I real-time quantitative PCR detection assay (RT-qPCR) for the detection of the porcine transmissible gastroenteritis virus (TGEV) using specific primers designed to amplify the highly conserved porcine TGEV S gene sequence. The threshold cycle (Ct) and the log plasmid copy numbers had a good linear relationship with
Li Cui, Congli Yuan, Xiuguo Hua
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Journal of Veterinary Diagnostic Investigation, 1999
The spike (S) glycoprotein of the Miller strain of transmissible gastroenteritis virus (TGEV) was recently cloned and expressed in baculovirus. The recombinant S protein was used as the coating antigen in a competition (blocking) enzyme-linked immunosorbent assay (ELISA) in combination with monoclonal antibodies to the S protein epitope A (conserved on
K, Sestak +3 more
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The spike (S) glycoprotein of the Miller strain of transmissible gastroenteritis virus (TGEV) was recently cloned and expressed in baculovirus. The recombinant S protein was used as the coating antigen in a competition (blocking) enzyme-linked immunosorbent assay (ELISA) in combination with monoclonal antibodies to the S protein epitope A (conserved on
K, Sestak +3 more
openaire +2 more sources
Monoclonal Antibodies in Immunodiagnosis & Immunotherapy, 2013
Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the ...
Qiong, Zhao +9 more
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Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the ...
Qiong, Zhao +9 more
openaire +2 more sources
Developmental & Comparative Immunology
Interferon-Induced protein 44-Like (IFI44L), a member of the interferon-stimulated genes (ISGs) family, plays a critical role in a variety of biological processes, particularly in antiviral defense and immune regulation. However, the role of human IFI44L in viral replication remains controversial.
Lilan Xie +3 more
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Interferon-Induced protein 44-Like (IFI44L), a member of the interferon-stimulated genes (ISGs) family, plays a critical role in a variety of biological processes, particularly in antiviral defense and immune regulation. However, the role of human IFI44L in viral replication remains controversial.
Lilan Xie +3 more
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Virology
TGEV mainly infects pig small intestinal epithelium, resulting in vomiting, diarrhea, dehydration, and death. IFIT3 is involved in resisting viral infection and is an innate immune regulator. In this study, we determined that TGEV infection could induce IFIT3 expression. The overexpression of IFIT3 inhibited TGEV infection, promoted the phosphorylation
Guohui Chen +13 more
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TGEV mainly infects pig small intestinal epithelium, resulting in vomiting, diarrhea, dehydration, and death. IFIT3 is involved in resisting viral infection and is an innate immune regulator. In this study, we determined that TGEV infection could induce IFIT3 expression. The overexpression of IFIT3 inhibited TGEV infection, promoted the phosphorylation
Guohui Chen +13 more
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2018
Oral inoculation of the porcine small intestine with live TGE virus has been shown to elicit a local cell-mediated response as well as a peripheral lymphocyte response. This has not been shown to occur following intramuscular (IM) administration of the pathogen.
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Oral inoculation of the porcine small intestine with live TGE virus has been shown to elicit a local cell-mediated response as well as a peripheral lymphocyte response. This has not been shown to occur following intramuscular (IM) administration of the pathogen.
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2003
Interferons are a family of molecules produced in response to a viral aggression. The author focuses here on alpha interferon, describing its biological properties (antiviral, immunomodulator, antitumoral and responsible for side effects such as fever), as well as its induction and production mechanisms.
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Interferons are a family of molecules produced in response to a viral aggression. The author focuses here on alpha interferon, describing its biological properties (antiviral, immunomodulator, antitumoral and responsible for side effects such as fever), as well as its induction and production mechanisms.
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Replication of Transmissible Gastroenteritis Coronavirus (TGEV) in Swine Alveolar Macrophages
Journal of General Virology, 1984H Laude, B Charley, J Gelfi
exaly
Antiviral Activity of Zinc Salts Against Transmissible Gastroenteritis Virus in Vitro
Veterinary Microbiology, 2012Zhanyong Wei +2 more
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