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Cold Spring Harbor Protocols, 2016
Identifying the genomic targets of transcription factors is an important step in understanding the regulatory networks of gene transcription in yeast. We have developed a method that utilizes what we refer to as transposon “calling cards,” in which a transcription factor directs the Ty5 retrotransposase to insert transposons into the genome adjacent to
David, Mayhew, Robi D, Mitra
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Identifying the genomic targets of transcription factors is an important step in understanding the regulatory networks of gene transcription in yeast. We have developed a method that utilizes what we refer to as transposon “calling cards,” in which a transcription factor directs the Ty5 retrotransposase to insert transposons into the genome adjacent to
David, Mayhew, Robi D, Mitra
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Trends in Ecology & Evolution, 1998
In his interesting commentary on transposable elements (TEs), gene silencing and macroevolution, John McDonald[1xMcDonald, J. Trends Ecol. Evol. 1998; 13: 94–95Abstract | Full Text PDF | PubMed | Scopus (52)See all References[1]omitted a major player that has acted in concert with TEs to drive vertebrate and plant evolution, namely, polyploidy.
M A, Matzke, A J, Matzke
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In his interesting commentary on transposable elements (TEs), gene silencing and macroevolution, John McDonald[1xMcDonald, J. Trends Ecol. Evol. 1998; 13: 94–95Abstract | Full Text PDF | PubMed | Scopus (52)See all References[1]omitted a major player that has acted in concert with TEs to drive vertebrate and plant evolution, namely, polyploidy.
M A, Matzke, A J, Matzke
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2009
Phage transduction is an attractive method of genetic manipulation in mycobacteria. PhiMycoMarT7 is well suited for transposon mutagenesis as it is temperature sensitive for replication and contains T7 promoters that promote transcription, a highly active transposase gene, and an Escherichia coli oriR6 K origin of replication.
M Sloan, Siegrist, Eric J, Rubin
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Phage transduction is an attractive method of genetic manipulation in mycobacteria. PhiMycoMarT7 is well suited for transposon mutagenesis as it is temperature sensitive for replication and contains T7 promoters that promote transcription, a highly active transposase gene, and an Escherichia coli oriR6 K origin of replication.
M Sloan, Siegrist, Eric J, Rubin
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Bacterial catabolic transposons
Applied Microbiology and Biotechnology, 1999The introduction of foreign organic hydrocarbons into the environment in recent years, as in the widespread use of antibiotics, has resulted in the evolution of novel adaptive mechanisms by bacteria for the biodegradation of the organic pollutants. Plasmids have been implicated in the catabolism of many of these complex xenobiotics. The catabolic genes
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Annual Review of Genetics, 2008
Tn5 was one of the first transposons to be identified ( 10 ). As a result of Tn5's early discovery and its simple macromolecular requirements for transposition, the Tn5 system has been a very productive tool for studying the molecular mechanism of DNA transposition. These studies are of broad value because they offer insights into DNA transposition in
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Tn5 was one of the first transposons to be identified ( 10 ). As a result of Tn5's early discovery and its simple macromolecular requirements for transposition, the Tn5 system has been a very productive tool for studying the molecular mechanism of DNA transposition. These studies are of broad value because they offer insights into DNA transposition in
openaire +2 more sources