Results 61 to 70 of about 49,276 (295)
Investigation into the mobile genetic elements of Clostridium difficile [PDF]
, 2013 Clostridium difficile is a pathogenic bacterium that can colonise both humans and various animals. Toxin production leads to clinical symptoms ranging from mild to severe diarrhoea and can result in potentially fatal pseudomembranous colitis.
Brouwer, MSM
core
Mobilization of giant piggyBac transposons in the mouse genome [PDF]
, 2011 The development of technologies that allow the stable delivery of large genomic DNA fragments in mammalian systems is important for genetic studies as well as for applications in gene therapy.
Zemin Ning +9 more
core +2 more sources
Engineering Microbial Particles for Next‐Generation Biomedical Platforms
Advanced Science, EarlyView.Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li +7 more
wiley +1 more source
PlantGFM: A Genomic Foundation Model for Discovery and Creation of Plant Genes
Advanced Science, EarlyView.A plant genomic foundation model pre‐trained on 12 species enables both accurate gene prediction and de novo gene design. Through AI‐human knowledge screening, seven designed sequences showed transcriptional activity in plants, with two expressing stable proteins—demonstrating the first DNA‐RNA‐protein expression of LLM‐generated genes in plants and ...
Changhao Li +10 more
wiley +1 more source
A virophage at the origin of large DNA transposons [PDF]
, 2011 DNA transposons are mobile genetic elements that have shaped the genomes of eukaryotes for millions of years, yet their origins remain obscure. We discovered a virophage that, on the basis of genetic homology, likely represents an evolutionary link ...
Fischer, M. +3 more
core +1 more source
Additional file 1 of Identification of transcriptionally active transposons in Barley [PDF]
, 2023 Supplementary Information: Table S1. The list of FLcDNAs related to transposons. Table S2. Seven intact barley transposons identified in this study. Table S3. Summary of primers used for RT-PCR analysis. Table S4.
Dongying Gao (123792) +1 more
core +1 more source
Frontiers in Microbiology, 2019 Acquisition of the blaNDM–1 gene by Proteus mirabilis is a concern because it already has intrinsic resistance to polymyxin E and tigecycline antibiotics. Here, we describe a P.
Dandan Dong +17 more
doaj +1 more source
Advanced Science, EarlyView.We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma +22 more
wiley +1 more source
mBio, 2019 Despite intensive study for 50 years, the biochemical and genetic links between lysine metabolism and central metabolism in Pseudomonas putida remain unresolved.
Mitchell G. Thompson +19 more
doaj +1 more source
Transposon for protein engineering [PDF]
Mobile Genetic Elements, 2016 Protein insertional fusion and circular permutation are 2 promising protein engineering techniques for creating integrated functionalities and sequence diversity of a protein, respectively. Finding insertion locations for protein insertional fusion and new termini for circular permutation through a rational approach is not always straightforward ...
Vandan, Shah, Jin Ryoun, Kim
openaire +2 more sources