Results 81 to 90 of about 49,131 (302)
mBio, 2018 Knowing the full set of essential genes for a given organism provides important information about ways to promote, and to limit, its growth and survival. For many non-model organisms, the lack of a stable haploid state and low transformation efficiencies
Ella Shtifman Segal +12 more
doaj +1 more source
Transposon for protein engineering [PDF]
Mobile Genetic Elements, 2016 Protein insertional fusion and circular permutation are 2 promising protein engineering techniques for creating integrated functionalities and sequence diversity of a protein, respectively. Finding insertion locations for protein insertional fusion and new termini for circular permutation through a rational approach is not always straightforward ...
Vandan, Shah, Jin Ryoun, Kim
openaire +2 more sources
Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants
Advanced Science, EarlyView.A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei +8 more
wiley +1 more source
Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics
Plants, 2019 Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated ...
Hasthi Ram +6 more
doaj +1 more source
Frequency of isolates having different transposons.
, 2016 Frequency of isolates having different transposons.
Malihe Talebi (834974) +7 more
core +1 more source
Advanced Science, EarlyView.IGF2BP1‐mediated m6A stabilization sustains SMC1A expression, enabling cohesin‐associated chromatin regulation of Nestin in hepatocellular carcinoma. This work reveals an epitranscriptomic‐chromatin‐cytoskeletal regulatory axis linked to malignant phenotypes and identifies SMC1A as a biologically relevant vulnerability in HCC.
Zhenxiang Peng +7 more
wiley +1 more source
Mobilome of Environmental Isolates of Clostridioides difficile
AntibioticsBackground/Objectives: Clostridioides difficile is a “One Health” pathogen and a cause of antibiotics-associated diarrhea and pseudomembranous colitis. Mobile genetic elements (MGEs) have been documented in the genomes of clinical C.
Khald Blau, Claudia Gallert
doaj +1 more source
mBio, 2019 Transposon mutagenesis is a widely applied and powerful genetic tool for the discovery of genes associated with selected phenotypes. Chlamydia trachomatis is a clinically significant, obligate intracellular bacterium for which many conventional genetic ...
Scott D. LaBrie +6 more
doaj +1 more source
Interactions of transposons with the cellular DNA repair machinery
, 2009 Transposons are ubiquitous components of both prokaryotic and eukaryotic genomes. Transposons are mobile genetic elements whose movement inherently involves the generation of various types of DNA damage in the genome.
Ivics, Z. +5 more
core +1 more source
PlantGFM: A Genomic Foundation Model for Discovery and Creation of Plant Genes
Advanced Science, EarlyView.A plant genomic foundation model pre‐trained on 12 species enables both accurate gene prediction and de novo gene design. Through AI‐human knowledge screening, seven designed sequences showed transcriptional activity in plants, with two expressing stable proteins—demonstrating the first DNA‐RNA‐protein expression of LLM‐generated genes in plants and ...
Changhao Li +10 more
wiley +1 more source