Results 81 to 90 of about 49,276 (295)

Transposon transgenesis in Xenopus [PDF]

Methods, 2010
Transposon-mediated integration strategies in Xenopus offer simple and robust methods for the generation of germline transgenic animals. Co-injection of fertilized one-cell embryos with plasmid DNA harboring a transposon transgene and synthetic mRNA encoding the cognate transposase enzyme results in mosaic integration of the transposon at early ...
Donald A, Yergeau, Clair M, Kelley, Haiqing, Zhu, Emin, Kuliyev, Paul E, Mead   +4 more
openaire   +2 more sources

Femtosecond‐Laser Nanocavitation Regenerates SERS‐Active Plasmonic Nanogaps for Longitudinal Molecular Sensing at Biointerfaces

Advanced Science, EarlyView.
A regenerative molecular sensing platform that co‐localizes surface‐enhanced Raman spectroscopy (SERS) sensing and nanocavitation‐based actuation. Femtosecond‐laser triggered nanocavitation produces thermomechanical forces to locally regenerate SERS‐active nanogaps in protein‐rich biofluids while preserving optical performance.
Aditya Garg, Ze Zong, Meitong Nie, Stacie E. Deaver, Elizabeth M. Van Order, Elieser Mejia, Peter Vikesland, Erin S. Gloag, Wei Zhou   +8 more
wiley   +1 more source

A superfamily of DNA transposons targeting multicopy small RNA genes. [PDF]

, 2013
Target-specific integration of transposable elements for multicopy genes, such as ribosomal RNA and small nuclear RNA (snRNA) genes, is of great interest because of the relatively harmless nature, stable inheritance and possible application for targeted ...
Kenji K Kojima, Kojima Kenji K., Kenji K. Kojima, Jerzy Jurka, Jerzy Jurka (9176), Jurka Jerzy, Kenji K. Kojima (207564)   +6 more
core   +1 more source

Mobilome of Environmental Isolates of Clostridioides difficile

Antibiotics
Background/Objectives: Clostridioides difficile is a “One Health” pathogen and a cause of antibiotics-associated diarrhea and pseudomembranous colitis. Mobile genetic elements (MGEs) have been documented in the genomes of clinical C.
Khald Blau, Claudia Gallert
doaj   +1 more source

Transposon Mutagenesis in Chlamydia trachomatis Identifies CT339 as a ComEC Homolog Important for DNA Uptake and Lateral Gene Transfer

mBio, 2019
Transposon mutagenesis is a widely applied and powerful genetic tool for the discovery of genes associated with selected phenotypes. Chlamydia trachomatis is a clinically significant, obligate intracellular bacterium for which many conventional genetic ...
Scott D. LaBrie, Zoë E. Dimond, Kelly S. Harrison, Srishti Baid, Jason Wickstrum, Robert J. Suchland, P. Scott Hefty   +6 more
doaj   +1 more source

MULTIDRUG RESISTANCE AND INTEGRONS IN ESCHERICHIA COLI ISOLATED FROM FOOD-PRODUCING ANIMALS IN SAUDI ARABIA [PDF]

Assiut Veterinary Medical Journal, 2015
Transference of antimicrobial resistance gene determinants by integrons is one of the important factors that can contribute to the increase in multidrug-resistant bacteria. The present study aimed to investigate 56 strains of animal-derived E.
SABRY A. HASSAN
doaj   +1 more source

Single‐cell Transcriptome Profiling Reveals Gene Regulatory Networks and Key Genes in the Root Epidermis and Cortical Cells Associated with Early Nodulation in Glycine Max

Advanced Science, EarlyView.
Single‐cell transcriptomics of soybean roots soon after rhizobial inoculation reveals epidermal and cortical cell‐specific programs and gene‐regulatory networks acting in symbiosis establishment. We identify an ethylene‐driven regulatory circuit involving WRKY6.3/6.4 transcription factors targeting select Nod19 genes that promotes infection‐thread ...
Yongbin Zhuang, Yu Geng, Xinyu Guo, Chen Wang, Yongjing Sun, Luqi Liu, Li Liu, QianQian Gao, XinFang Zhu, Hongxiang Cao, Gengshuo Zhang, Yunxia Liu, Guobin Zhang, Baoyin Chen, Jinfei Zhang, Xin Hou, Xiaoming Li, Dajian Zhang   +17 more
wiley   +1 more source

The Caenorhabditis briggsae genome contains active CbmaT1 and Tcb1 transposons [PDF]

, 2005
The maT clade of transposons is a group of transposable elements intermediate in sequence and predicted protein structure to mariner and Tc transposons, with a distribution thus far limited to a few invertebrate species.
Johnson, N., Whyard, S., Johnson, N. M., Brownlie, J., Brownlie, J. C.   +4 more
core   +1 more source

Transposon-directed insertion-site sequencing (TraDIS) analysis of Enterococcus faecium using nanopore sequencing and a WebAssembly analysis platform

Microbiology Spectrum
Vancomycin-resistant Enterococcus faecium (VREfm) are healthcare-associated opportunistic pathogens of global significance. Genetic tools are needed to understand the molecular basis for VREfm clinically relevant phenotypes, such as persistence within ...
Alexandra L. Krause, Wytamma Wirth, Adrianna M. Turner, Louise Judd, Lucy Li, Willem van Schaik, Benjamin P. Howden, Glen P. Carter, Torsten Seemann, Ryan Wick, Timothy P. Stinear, Andrew H. Buultjens, Ian R. Monk   +12 more
doaj   +1 more source

A Pan‐Methylome Framework for Population‐Scale Bacterial Epigenomics

Advanced Science, EarlyView.
A scalable quantitative framework unlocks population‐level comparative epigenomics in bacteria. By transforming site‐level data into standardized traits, this approach reconstructs methylation‐informed phylogenies and defines the core epigenome.
Bin Ma, Huimin Gong, Ran Zhuo, Zaixiao Rao, Yanxi Wan, Mingyang Wang, Zahra Zeinalzadeh, Yuan Gao, Aohan Guan, Xinrun Li, Wanting Chen, Xiliang Wang, Yun Wan, Sihua Zhang, Yuncai Xiao, Qi Huang, Paul R. Langford, Xiangru Wang, Rui Zhou, Rui Luo, Hongbo Zhou, Chengliang Zhu, Hui Jin   +22 more
wiley   +1 more source