An Optimized RNA Extraction Method From Micro-quantities of Guinea Pig Cartilage and Synovium for Osteoarthritis Research [PDF]
Bio-ProtocolOsteoarthritis (OA) is the primary cause of joint impairment, particularly in the knee. The prevalence of OA has significantly increased, with knee OA being a major contributor whose pathogenesis remains unknown. Articular cartilage and the synovium play
Nidhi Bhardwaj +2 more
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LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer [PDF]
Journal of Cell and Molecular Research, 2021 Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis.
Seyedeh Nahid Fotuhi +2 more
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Frontiers in Agronomy, 2022 Citrus germplasm programs can benefit from high-throughput polymerase chain reaction (PCR)-based methods for the detection of graft-transmissible pathogens in propagative materials.
Tyler Dang +32 more
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Rodriguésia, 2020 Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha +4 more
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High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
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First report of tilapia lake virus emergence in fish farms in the department of Córdoba, Colombia [PDF]
Veterinary World, 2021 Background and Aim: In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the
Héctor Contreras +5 more
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COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES
Revista Brasileira de Fruticultura, 2016 Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to
ANNY CAROLYNE DA LUZ +2 more
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Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology
Acta Stomatologica Croatica, 2016 Objective of work: The aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real ...
Mônica Ghislaine Oliveira Alves +9 more
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Alkaline water modules the expression of inflammation genes: in vitro model for cancer prevention [PDF]
World Cancer Research Journal, 2020 OBJECTIVE: Many researches focus their attention on the behavior of cells to specific substances with alkalizing power. Each substance that we introduce with food, as well as water, contributes to conditioning the electrophilic balance (acid-base) of the
A. Del Buono +5 more
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pH-sensing G protein-coupled orphan receptor GPR68 is expressed in human cartilage and correlates with degradation of extracellular matrix during OA progression [PDF]
PeerJ, 2023 Background Osteoarthritis (OA) is a debilitating joints disease affecting millions of people worldwide. As OA progresses, chondrocytes experience heightened catabolic activity, often accompanied by alterations in the extracellular environment’s ...
Nazir M. Khan +5 more
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