Strategies for Validation of Inactivation of Viruses with Trizol® LS and Formalin Solutions. [PDF]
Appl Biosaf, 2020 Introduction: Inactivation of biological agents and particularly select agents has come under increased scrutiny since the US Army inadvertently shipped live anthrax both inside and outside the US, leading to more stringent regulations regarding ...
Retterer C +8 more
europepmc +4 more sources
Efficient recovery of proteins from multiple source samples after TRIzol(®) or TRIzol(®)LS RNA extraction and long-term storage. [PDF]
BMC Genomics, 2013 BackgroundSimultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often
Simões AE +11 more
europepmc +5 more sources
Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™ [PDF]
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
Zymo Research Corporation +1 more
doaj +2 more sources
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
doaj +2 more sources
An Optimized RNA Extraction Method From Micro-quantities of Guinea Pig Cartilage and Synovium for Osteoarthritis Research [PDF]
Bio-ProtocolOsteoarthritis (OA) is the primary cause of joint impairment, particularly in the knee. The prevalence of OA has significantly increased, with knee OA being a major contributor whose pathogenesis remains unknown. Articular cartilage and the synovium play
Nidhi Bhardwaj +2 more
doaj +2 more sources
LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer [PDF]
Journal of Cell and Molecular Research, 2021 Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis.
Seyedeh Nahid Fotuhi +2 more
doaj +1 more source
Frontiers in Agronomy, 2022 Citrus germplasm programs can benefit from high-throughput polymerase chain reaction (PCR)-based methods for the detection of graft-transmissible pathogens in propagative materials.
Tyler Dang +32 more
doaj +1 more source
Rodriguésia, 2020 Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha +4 more
doaj +1 more source
A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia [PDF]
, 2020 © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Arenas Gomez, C. M., Woodcock, M. R., Smith, J. J., Voss, S. R., & Delgado, J. P. A de novo reference
Arenas Gómez, Claudia Marcela +4 more
core +1 more source
First report of tilapia lake virus emergence in fish farms in the department of Córdoba, Colombia [PDF]
Veterinary World, 2021 Background and Aim: In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the
Héctor Contreras +5 more
doaj +1 more source