Results 1 to 10 of about 111,207 (280)
Efficient recovery of proteins from multiple source samples after trizol® or trizol®LS RNA extraction and long-term storage [PDF]
BMC Genomics, 2013 BackgroundSimultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often
André E. Simões +11 more
semanticscholar +4 more sources
Strategies for Validation of Inactivation of Viruses with Trizol® LS and Formalin Solutions [PDF]
Appl Biosaf, 2020 Introduction: Inactivation of biological agents and particularly select agents has come under increased scrutiny since the US Army inadvertently shipped live anthrax both inside and outside the US, leading to more stringent regulations regarding ...
Cary Retterer +8 more
semanticscholar +4 more sources
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
doaj +2 more sources
Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™ [PDF]
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
doaj +2 more sources
BioTechniques, 2007 A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic ...
Amanda B. Hummon +3 more
doaj +2 more sources
, 2011 Resorbable bioceramics are attractive for medical applications such as bone substitution. Biochemical analysis on cells cultured on these biomaterials is vital to predict the impact of the materials in vivo and RNA extraction is an essential step in gene
Juliana Tsz Yan Lee +2 more
openalex +2 more sources
LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer [PDF]
Journal of Cell and Molecular Research, 2021 Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis.
Seyedeh Nahid Fotuhi +2 more
doaj +1 more source
Frontiers in Agronomy, 2022 Citrus germplasm programs can benefit from high-throughput polymerase chain reaction (PCR)-based methods for the detection of graft-transmissible pathogens in propagative materials.
Tyler Dang +32 more
doaj +1 more source
A TRIzol-based method for high recovery of plasma sncRNAs approximately 30 to 60 nucleotides
Scientific Reports, 2022 Protein functional effector sncRNAs (pfeRNAs) are approximately 30–60 nucleotides (nt), of which the extraction method from plasma has not yet been reported.
Kristen Rodgers +6 more
semanticscholar +1 more source
Post-trizol protein extraction from peripheral blood mononuclear cells
Journal of the Serbian Chemical Society, 2023 After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation.
Jovana Stevanović +3 more
semanticscholar +1 more source