Results 11 to 20 of about 114,931 (280)

Isolation and solubilization of proteins after TRIzol® extraction of RNA and DNA from patient material following prolonged storage

open access: yesBioTechniques, 2007
A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic ...
Amanda B. Hummon   +3 more
doaj   +2 more sources

Modification of the Trizol Method for the Extraction of RNA from Prorocentrum triestinum ACIZ_LEM2. [PDF]

open access: yesInt J Mol Sci
In samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very ...
Huarachi-Olivera R   +10 more
europepmc   +2 more sources

A TRIzol-based method for high recovery of plasma sncRNAs approximately 30 to 60 nucleotides

open access: yesScientific Reports, 2022
Protein functional effector sncRNAs (pfeRNAs) are approximately 30–60 nucleotides (nt), of which the extraction method from plasma has not yet been reported.
Kristen Rodgers   +6 more
semanticscholar   +1 more source

Post-trizol protein extraction from peripheral blood mononuclear cells

open access: yesJournal of the Serbian Chemical Society, 2023
After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation.
Jovana Stevanović   +3 more
semanticscholar   +1 more source

Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection program

open access: yesPLoS ONE, 2021
The use of saliva for the diagnosis of SARS-CoV-2 has shown to be a good alternative to nasopharyngeal swabs (NPS), since it permits self-collection, avoids the exposure of healthy persons to infected patients, reduces waiting times, eliminates the need ...
Haydee Miranda-Ortíz   +15 more
semanticscholar   +1 more source

An Optimised TRIzol-based Protocol for the Improvement of RNA Extraction Yield of Tomato Stem

open access: yesPertanika Journal of Tropical Agricultural Science, 2021
One of the most common methods for purifying RNA is using TRIzol reagent because of its simplicity and economic feasibility. However, the drawback of this method is frequently the low quality of extracted RNA due to contaminants from the residue of ...
Anis Afifah   +5 more
semanticscholar   +1 more source

COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES

open access: yesRevista Brasileira de Fruticultura, 2016
Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to
ANNY CAROLYNE DA LUZ   +2 more
doaj   +1 more source

Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology

open access: yesActa Stomatologica Croatica, 2016
Objective of work: The aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real ...
Mônica Ghislaine Oliveira Alves   +9 more
doaj   +1 more source

Phenolic metabolites of anthocyanins modulate mechanisms of endothelial function [PDF]

open access: yes, 2015
Anthocyanins are reported to have vascular bioactivity, however their mechanisms of action are largely unknown. Evidence suggests that anthocyanins modulate endothelial function, potentially by increasing nitric oxide (NO) synthesis, or enhancing NO ...
Cassidy, Aedin   +4 more
core   +1 more source

Alveolar macrophages and Toll-like receptor 4 mediate ventilated lung ischemia reperfusion injury in mice. [PDF]

open access: yes, 2012
BackgroundIschemia-reperfusion (I-R) injury is a sterile inflammatory process that is commonly associated with diverse clinical situations such as hemorrhage followed by resuscitation, transient embolic events, and organ transplantation.
Dodd-o, Jeffrey M   +5 more
core   +1 more source

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