Results 11 to 20 of about 111,207 (280)

An Optimized RNA Extraction Method From Micro-quantities of Guinea Pig Cartilage and Synovium for Osteoarthritis Research [PDF]

open access: yesBio-Protocol
Osteoarthritis (OA) is the primary cause of joint impairment, particularly in the knee. The prevalence of OA has significantly increased, with knee OA being a major contributor whose pathogenesis remains unknown. Articular cartilage and the synovium play
Nidhi Bhardwaj   +2 more
doaj   +2 more sources

Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection program

open access: yesPLoS ONE, 2021
The use of saliva for the diagnosis of SARS-CoV-2 has shown to be a good alternative to nasopharyngeal swabs (NPS), since it permits self-collection, avoids the exposure of healthy persons to infected patients, reduces waiting times, eliminates the need ...
Haydee Miranda-Ortíz   +15 more
semanticscholar   +1 more source

An efficient method for total RNA extraction from leaves of arboreal species from the Brazilian Cerrado

open access: yesRodriguésia, 2020
Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha   +4 more
doaj   +1 more source

An Optimised TRIzol-based Protocol for the Improvement of RNA Extraction Yield of Tomato Stem

open access: yesPertanika Journal of Tropical Agricultural Science, 2021
One of the most common methods for purifying RNA is using TRIzol reagent because of its simplicity and economic feasibility. However, the drawback of this method is frequently the low quality of extracted RNA due to contaminants from the residue of ...
Anis Afifah   +5 more
semanticscholar   +1 more source

First report of tilapia lake virus emergence in fish farms in the department of Córdoba, Colombia [PDF]

open access: yesVeterinary World, 2021
Background and Aim: In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the
Héctor Contreras   +5 more
doaj   +1 more source

COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES

open access: yesRevista Brasileira de Fruticultura, 2016
Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to
ANNY CAROLYNE DA LUZ   +2 more
doaj   +1 more source

Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology

open access: yesActa Stomatologica Croatica, 2016
Objective of work: The aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real ...
Mônica Ghislaine Oliveira Alves   +9 more
doaj   +1 more source

Alkaline water modules the expression of inflammation genes: in vitro model for cancer prevention [PDF]

open access: yesWorld Cancer Research Journal, 2020
OBJECTIVE: Many researches focus their attention on the behavior of cells to specific substances with alkalizing power. Each substance that we introduce with food, as well as water, contributes to conditioning the electrophilic balance (acid-base) of the
A. Del Buono   +5 more
doaj   +1 more source

Alveolar macrophages and Toll-like receptor 4 mediate ventilated lung ischemia reperfusion injury in mice. [PDF]

open access: yes, 2012
BackgroundIschemia-reperfusion (I-R) injury is a sterile inflammatory process that is commonly associated with diverse clinical situations such as hemorrhage followed by resuscitation, transient embolic events, and organ transplantation.
Dodd-o, Jeffrey M   +5 more
core   +1 more source

Phenolic metabolites of anthocyanins modulate mechanisms of endothelial function [PDF]

open access: yes, 2015
Anthocyanins are reported to have vascular bioactivity, however their mechanisms of action are largely unknown. Evidence suggests that anthocyanins modulate endothelial function, potentially by increasing nitric oxide (NO) synthesis, or enhancing NO ...
Cassidy, Aedin   +4 more
core   +1 more source

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