Results 11 to 20 of about 116,911 (276)

High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction

BioTechniques, 2020
Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen, Ivan J Vechetti, Taylor R Valentino, John J McCarthy   +3 more
doaj   +2 more sources

An Optimized RNA Extraction Method From Micro-quantities of Guinea Pig Cartilage and Synovium for Osteoarthritis Research [PDF]

Bio-Protocol
Osteoarthritis (OA) is the primary cause of joint impairment, particularly in the knee. The prevalence of OA has significantly increased, with knee OA being a major contributor whose pathogenesis remains unknown. Articular cartilage and the synovium play
Nidhi Bhardwaj, Diksha Rana, Jyotdeep Kaur   +2 more
doaj   +2 more sources

Isolation and solubilization of proteins after TRIzol® extraction of RNA and DNA from patient material following prolonged storage

BioTechniques, 2007
A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic ...
Amanda B. Hummon, Sharlene R. Lim, Michael J. Difilippantonio, Thomas Ried   +3 more
doaj   +2 more sources

Strategies for Validation of Inactivation of Viruses with Trizol® LS and Formalin Solutions. [PDF]

Appl Biosaf, 2020
Introduction: Inactivation of biological agents and particularly select agents has come under increased scrutiny since the US Army inadvertently shipped live anthrax both inside and outside the US, leading to more stringent regulations regarding ...
Retterer C, Kenny T, Zamani R, Altamura LA, Kearney B, Jaissle J, Coyne S, Olschner S, Harbourt D.   +8 more
europepmc   +2 more sources

Efficient recovery of proteins from multiple source samples after TRIzol(®) or TRIzol(®)LS RNA extraction and long-term storage. [PDF]

BMC Genomics, 2013
BackgroundSimultaneous isolation of nucleic acids and proteins from a single biological sample facilitates meaningful data interpretation and reduces time, cost and sampling errors. This is particularly relevant for rare human and animal specimens, often
Simões AE, Pereira DM, Amaral JD, Nunes AF, Gomes SE, Rodrigues PM, Lo AC, D'Hooge R, Steer CJ, Thibodeau SN, Borralho PM, Rodrigues CM.   +11 more
europepmc   +3 more sources

LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer [PDF]

Journal of Cell and Molecular Research, 2021
Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis.
Seyedeh Nahid Fotuhi, Mohammad Khalaj-Kondori, Hadis Karimi   +2 more
doaj   +1 more source

A comparative analysis of RNA isolation methods optimized for high-throughput detection of viral pathogens in California’s regulatory and disease management program for citrus propagative materials

Frontiers in Agronomy, 2022
Citrus germplasm programs can benefit from high-throughput polymerase chain reaction (PCR)-based methods for the detection of graft-transmissible pathogens in propagative materials.
Tyler Dang, Sohrab Bodaghi, Fatima Osman, Jinbo Wang, Jinbo Wang, Tavia Rucker, Shih-Hua Tan, Amy Huang, Deborah Pagliaccia, Stacey Comstock, Irene Lavagi-Craddock, Kiran R. Gadhave, Kiran R. Gadhave, Paulina Quijia-Lamina, Arunabha Mitra, Brandon Ramirez, Gerardo Uribe, Alexandra Syed, Sarah Hammado, Iman Mimou, Roya Campos, Silva Abdulnour, Michael Voeltz, Jinhwan Bae, Emily Dang, Brittany Nguyen, Xingyu Chen, Noora Siddiqui, Yi Tien Hsieh, Shurooq Abu-Hajar, Joshua Kress, Kristina Weber, Georgios Vidalakis   +32 more
doaj   +1 more source

Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection program

PLoS ONE, 2021
The use of saliva for the diagnosis of SARS-CoV-2 has shown to be a good alternative to nasopharyngeal swabs (NPS), since it permits self-collection, avoids the exposure of healthy persons to infected patients, reduces waiting times, eliminates the need ...
Haydee Miranda-Ortíz, E. A. Fernández-Figueroa, E. Ruíz-García, A. Muñoz-Rivas, A. Méndez-Pérez, J. Mendez-Galvan, H. Astudillo-de la Vega, Belem Gabiño-López, Ricardo Nava-Monroy, Alejandro López-Flores a la Torre, Tanit López-Vergara Anaya, D. Vilar-Compte, Uriel Coquis-Navarrete, Melissa Valdés-Reyes, S. Sánchez-Montes, I. Becker   +15 more
semanticscholar   +1 more source

Modification of the Trizol Method for the Extraction of RNA from Prorocentrum triestinum ACIZ_LEM2. [PDF]

Int J Mol Sci
In samples of harmful algal blooms (HABs), seawater can contain a high abundance of microorganisms and elemental ions. Along with the hardness of the walls of key HAB dinoflagellates such as Prorocentrum triestinum, this makes RNA extraction very ...
Huarachi-Olivera R, Teresa Mata M, Ardiles-Candia A, Escobar-Méndez V, Gatica-Cortes C, Ahumada M, Orrego J, Vidal-Veuthey B, Cárdenas JP, González L, Riquelme C.   +10 more
europepmc   +2 more sources

Post-trizol protein extraction from peripheral blood mononuclear cells

Journal of the Serbian Chemical Society, 2023
After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation.
Jovana Stevanović, D. Robajac, O. Nedić, Zorana Dobrijević   +3 more
semanticscholar   +1 more source