Results 41 to 50 of about 764 (211)

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source

Nanopore sequencing of intact aminoacylated tRNAs

Nature Communications
The intricate landscape of tRNA modification presents persistent analytical challenges, which have impeded efforts to simultaneously resolve sequence, modification, and aminoacylation state at the level of individual tRNAs.
Laura K. White, Aleksandar Radakovic, Marcin P. Sajek, Kezia Dobson, Kent A. Riemondy, Samantha del Pozo, Jack W. Szostak, Jay R. Hesselberth   +7 more
doaj   +1 more source

Integrated genomic analysis reveals regulatory pathways and dynamic landscapes of the tRNA transcriptome

Scientific Reports, 2021
tRNAs and tRNA-derived RNA fragments (tRFs) play various roles in many cellular processes outside of protein synthesis. However, comprehensive investigations of tRNA/tRF regulation are rare.
Zefang Sun, Jia Tan, Minqiong Zhao, Qiyao Peng, Mingqing Zhou, Shanru Zuo, Feilong Wu, Xueguang Li, Yangyang Dong, Ming Xie, Yide Yang, Junhua Zhou, Xianghua Liu, Quanze He, Zuping He, Xing Yu, Quanyuan He   +16 more
doaj   +1 more source

Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2

Microbial Cell, 2022
In yeast, Elongator-dependent tRNA modifications are regulated by the Kti11•Kti13 dimer and hijacked for cell killing by zymocin, a tRNase ribotoxin. Kti11 (alias Dph3) also controls modification of elongation factor 2 (EF2) with diphthamide, the target ...
Meike Arend, Koray Ütkür, Harmen Hawer, Klaus Mayer, Namit Ranjan, Lorenz Adrian, Ulrich Brinkmann, Raffael Schaffrath   +7 more
doaj   +1 more source

Epidermal METTL1‐Mediated m7G Modification Drives Psoriatic Inflammation by Stabilizing Bdkrb1 and Orchestrating Neutrophil Recruitment

Advanced Science, EarlyView.
This study unveils an unrecognized pro‐inflammatory epitranscriptomic checkpoint in psoriasis. By installing m7G modifications on the 5′ UTR of Bdkrb1 mRNA, METTL1 enhances receptor stability to orchestrate keratinocyte‐driven neutrophil recruitment via p38 MAPK signaling.
Chang Zhang, Jiayi Lu, Sihao Yan, Yirui Wang, Zhuo Li, Weiwei Chen, Yang Han, Ziyan Zhang, Yafen Yu, Qi Zhen, Liangdan Sun   +10 more
wiley   +1 more source

Preparation of Yeast tRNA Sample for NMR Spectroscopy

Bio-Protocol, 2020
Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs.
Marjorie Catala, Alexandre Gato, Carine Tisné, Pierre Barraud   +3 more
doaj   +1 more source

The Evolution and Implications of the Inosine tRNA Modification

Journal of Molecular Biology
Ever since the legendary publication by Francis Crick in JMB introducing the wobble hypothesis in 1966, inosine has been a permanent part of molecular biology. This review aims to integrate the rich array of novel insights emerging from subsequent research on the adenine-to-inosine modification of tRNA, with an emphasis on the results obtained during ...
P.T.S. van der Gulik (Peter), W.D. Hoff (Wouter)   +1 more
openaire   +3 more sources

Targeting Dnmt3a/m5C/RelA Axis Attenuates Microglia Inflammatory Response and Improves Postoperative Recovery in Chronic Compressive Cervical Spinal Cord Injury

Advanced Science, EarlyView.
A pro‐inflammatory microglial subset persists after decompression in chronic compressive cervical spinal cord injury and shows enhanced Dnmt3a‐driven m5C signaling. By stabilizing RelA mRNA, this axis sustains NF‐κB activation and postoperative neuroinflammation.
Tianyu Qin, Yuan Jiang, Yongheng Xie, Huanwei Qu, Wenbin Yan, Jingle Chen, Yining Chen, Huayi Li, Naibo Feng, Jiajun Wu, Chao Zhang, Zhengqi Huang, Ming Shi, Zhihuai Deng, Guozhi Xiao, Houqing Long   +15 more
wiley   +1 more source

Identification of D Modification Sites by Integrating Heterogeneous Features in Saccharomyces cerevisiae

Molecules, 2019
As an abundant post-transcriptional modification, dihydrouridine (D) has been found in transfer RNA (tRNA) from bacteria, eukaryotes, and archaea. Nonetheless, knowledge of the exact biochemical roles of dihydrouridine in mediating tRNA function is still
Pengmian Feng, Zhaochun Xu, Hui Yang, Hao Lv, Hui Ding, Li Liu   +5 more
doaj   +1 more source

Amino acid modifications on tRNA.

Acta biochimica et biophysica Sinica, 2008
The accurate formation of cognate aminoacyl-transfer RNAs (aa-tRNAs) is essential for the fidelity of translation. Most amino acids are esterified onto their cognate tRNA isoacceptors directly by aa-tRNA synthetases. However, in the case of four amino acids (Gln, Asn, Cys and Sec), aminoacyl-tRNAs are made through indirect pathways in many organisms ...
Yuan, J. ; https://orcid.org/0000-0003-1219-3316, Sheppard, K., Soll, D.   +2 more
openaire   +2 more sources