Results 51 to 60 of about 3,697 (189)

Activating Mutations Reveal a Role of TRPML1 in Lysosomal Exocytosis [PDF]

Biophysical Journal, 2010
The contents of lysosomes undergo exocytosis (lysosomal exocytosis) in response to an increase of intracellular Ca2+. Emerging evidence suggests that lysosomal exocytosis plays important roles in a variety of cell biological functions including neurotransmitter release, neurite outgrowth, and plasma membrane repair.
Wang, Xiang, Dong, Xian-ping, Shen, Dongbiao, Xu, Haoxing   +3 more
openaire   +1 more source

Modulation of Lysosomal Cl⁻ Mediates Migration and Apoptosis through the TRPML1 as a Lysosomal Cl⁻ Sensor

Cells, 2023
Lysosomes are responsible for protein degradation and clearance in cellular recycling centers. It has been known that the lysosomal chloride level is enriched and involved in the intrinsic lysosomal function.
Dongun Lee, Jeong Hee Hong
doaj   +1 more source

Ammonia Accumulation Drives Intervertebral Disc Degeneration by Triggering Ammonia‐Induced Cell Death Through Lysosome–Mitochondria Crosstalk

Cell Proliferation, EarlyView.
Mechanical strain disrupts the lysosome‐mitochondria axis via GLS1‐mediated ammonia toxicity, causing NP cell death and ECM breakdown in IVDD. CB839‐mediated GLS1 inhibition effectively rescues cellular homeostasis and alleviates disc degeneration.
Yang Zhang, Yucheng Gao, Shuhang Dong, Shuanggong Liu, Lei Liu, Ziyi Song, Jie Xu, Yijun Rong, Guangxu Song, Yingze Zhang, Dongjin Wu, Zengxin Gao   +11 more
wiley   +1 more source

Activating Mutations of the TRPML1 Channel Revealed by Proline-scanning Mutagenesis [PDF]

Journal of Biological Chemistry, 2009
The mucolipin TRP (TRPML) proteins are a family of endolysosomal cation channels with genetically established importance in humans and rodent. Mutations of human TRPML1 cause type IV mucolipidosis, a devastating pediatric neurodegenerative disease. Our recent electrophysiological studies revealed that, although a TRPML1-mediated current can only be ...
Xian-ping, Dong, Xiang, Wang, Dongbiao, Shen, Su, Chen, Meiling, Liu, Yanbin, Wang, Eric, Mills, Xiping, Cheng, Markus, Delling, Haoxing, Xu   +9 more
openaire   +2 more sources

Mitophagy in skeletal muscle: Impact of ageing, exercise and disuse

Experimental Physiology, EarlyView.
Abstract Skeletal muscle plays an important role in whole‐body health, quality of life and regulation of metabolism. The maintenance of a healthy mitochondrial pool is imperative for the preservation of skeletal muscle quality and is mediated through mitochondrial quality control consisting of mitochondrial turnover mediated by a balance between ...
Anastasiya Kuznyetsova, David A. Hood
wiley   +1 more source

Pathophysiological Role of Transient Receptor Potential Mucolipin Channel 1 in Calcium-Mediated Stress-Induced Neurodegenerative Diseases

Frontiers in Physiology, 2020
Mucolipins (TRPML) are endosome/lysosome Ca2+ permeable channels belonging to the family of transient receptor potential channels. In mammals, there are three TRPML proteins, TRPML1, 2, and 3, encoded by MCOLN1-3 genes.
Giorgio Santoni, Federica Maggi, Federica Maggi, Consuelo Amantini, Oliviero Marinelli, Oliviero Marinelli, Massimo Nabissi, Maria Beatrice Morelli, Maria Beatrice Morelli   +8 more
doaj   +1 more source

Energetic microdomains and the vascular control of neuronal and muscle excitability: Toward a unified model

The Journal of Physiology, EarlyView.
Abstract figure legend The capillary–mitochondria–ion channel (CMIC) axis scales structural resources to match functional workload. (Left) In settings of restricted energetic capacity (e.g. cortical neurons), sparse capillary networks and modest mitochondrial pools set a lower energetic ceiling, sufficient to support phasic, low‐workload excitability. (
L. Fernando Santana, Scott Earley
wiley   +1 more source

TRPML1 channels initiate Ca2+ sparks in vascular smooth muscle cells

, 2020
TRPML1 (transient receptor potential mucolipin 1) is a Ca2+-permeable, nonselective cation channel localized to the membranes of endosomes and lysosomes and is not present or functional on the plasma membrane.
Pritchard, Harry A T; id_orcid, Sanders, Kenton M, Drumm, Bernard T, Thakore, Pratish, Griffin, Caoimhin S, Yamasaki, Evan, Lane, Conor, Feng Earley, Yumei, Earley, Scott   +8 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, Volume 600, Issue 17, Page 2564-2578, September 2026.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Rap and rapalogs bind TRPML1 in vitro.

, 2019
(A) Lack of FK506 effect on ITRPML1. Representative ITRPML1 was shown. (B) Rap bound to immuno-purified EGFP-TRPML1 immobilized on Pro-A biosensors in a dose-dependent manner. (C) Dose-dependent Tem-TRPML1 binding. (D) Weak or nonspecific binding of Zota
Rong Shao (202747), Nirakar Sahoo (1923211), Chenlang Gao (6718490), Xiaoli Zhang (135098), Dan Li (106345), Qiong Gao (13880), Ken Inoki (6718493), Tadashi Eguchi (1388379), Wei Chen (23863), Han Zhao (130768), Fumitaka Kudo (1388382), Xueni Yan (6718484), Shiyi Wang (6066389), Haoxing Xu (70454), Benfang Ruan (6718496), Yao Yao (335996), Meimei Yang (6718487), Junsheng Yang (606741), Lin Su (548272)   +18 more
core   +1 more source