Results 101 to 110 of about 736,972 (389)

Cis-mediated down-regulation of a trypsin gene associated with Bt resistance in cotton bollworm

Scientific Reports, 2014
Transgenic plants producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are useful for pest control, but their efficacy is reduced when pests evolve resistance.
Chenxi Liu, Yutao Xiao, Xianchun Li, B. Oppert, B. Tabashnik, Kongming Wu   +5 more
semanticscholar   +1 more source

Development of 4T1 breast cancer mouse model system for preclinical carbonic anhydrase IX studies

FEBS Open Bio, EarlyView.
Carbonic anhydrase IX (CAIX) is a well‐recognised therapeutic target and prognostic biomarker in cancer. We developed and characterised a robust murine breast cancer model system that is suitable for CAIX studies in vitro and in vivo—it comprises both CAIX‐positive and CAIX‐negative controls and provides a solid platform for the comprehensive ...
Zane Kalniņa, Ilva Liekniņa, Svetlana Koteloviča, Ramona Petrovska, Gediminas Žvinys, Agne Petrosiute, Asta Zubrienė, Matīss Toms Laugalis, Vendija Skeltona, Juris Jansons, Madara Kreishmane, Edita Čapkauskaitė, Daumantas Matulis, Kaspars Tārs   +13 more
wiley   +1 more source

Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. [PDF]

, 2017
Small ubiquitin-like modifier (SUMO) modification regulates numerous cellular processes. Unlike ubiquitin, detection of endogenous SUMOylated proteins is limited by the lack of naturally occurring protease sites in the C-terminal tail of SUMO proteins ...
Ahmad, Alla S, Bennett, Eric J, Clauser, Karl R, Gu, Hongbo, Komives, Elizabeth A, Leonard, Marilyn, Lumpkin, Ryan J, Meyer, Jesse G, Zhu, Yiying   +8 more
core   +2 more sources

Knockout of the mitoribosome rescue factors Ict1 or Mtrfr is viable in zebrafish but not mice: compensatory mechanisms underlying each factor's loss

FEBS Open Bio, EarlyView.
Mitochondria contain two mitoribosome rescue factors, ICT1 and MTRFR (C12orf65). ICT1 also functions as a mitoribosomal protein in mice and humans, and its loss is lethal. Although Mtrfr knockout mice could not be generated, knockout zebrafish lines for ict1 and mtrfr were established.
Nobukazu Nameki, Chika Tomisawa, Soichiro Hoshino, Hidehiko Shimizu, Masashi Abe, Sho Arai, Kanako Kuwasako, Naoki Asakawa, Yusuke Inoue, Takuro Horii, Izuho Hatada, Masakatsu Watanabe   +11 more
wiley   +1 more source

The Spreading of Pepsin and of Trypsin [PDF]

Journal of General Physiology, 1935
In this article the behavior of pepsin and trypsin in a monolayer under various conditions will be described. Both substances1 show all the characteristic properties of other proteins. Pepsin is admirably suited for the study of the influence of positive ions, as its isoelectric point lies far to the acid side. On trypsin the influence of negative ions
openaire   +3 more sources

Proteolytic Activation of the Human Epithelial Sodium Channel by Trypsin IV and Trypsin I Involves Distinct Cleavage Sites*

Journal of Biological Chemistry, 2014
Background: Proteolysis is required for ENaC activity, but the proteases activating ENaC in epithelial tissues are unknown. Results: Human trypsin IV and trypsin I activate ENaC by cleavage at distinct sites in the channel's γ-subunit.
S. Haerteis, Annabel Krappitz, M. Krappitz, Jane E. Murphy, M. Bertog, B. Krueger, Regina Nacken, Hyunjae Chung, M. Hollenberg, W. Knecht, N. Bunnett, C. Korbmacher   +11 more
semanticscholar   +1 more source

Short peptide perturbs spermatogenesis via immune microenvironment dysregulation and mitochondrial imbalance

FEBS Open Bio, EarlyView.
In the blood–testis barrier, occludin is crucial for tight junctions. This study demonstrates that occludin‐targeting short peptides disrupt junction integrity, inducing immune cell infiltration, tumor necrosis factor‐α/interleukin‐6 secretion and mitochondrial dysfunction, ultimately triggering apoptosis.
Heng Wang, Xiaofang Tan, Deyu Chen
wiley   +1 more source

Investigation of Mechanisms of Interaction of Yersinia pestis EV NIIEG Vaccine Strain Bacteria with Human Red Blood Cells

Проблемы особо опасных инфекций, 2012
The comparative experiments demonstrated strong correlation between the index of Yersinia pestis EV NIIEG strain adhesion to human red blood cells and hydrophobic properties of bacteria.
E. V. Pimenov, V. A. Oborin, A. G. Ivonin   +2 more
doaj   +1 more source

Preparation and characterization of renal cell peptides from fetal rats for their antitumor activity

FEBS Open Bio, EarlyView.
This study aimed to prepare renal cells (RCs) from fetal rats which were digested by enzymes. Candidate peptides RCPs were characterized by capillary HPLC and MS and their bioactivity was predicted using peptideranker. The predicted top 10 bioactive peptides were synthesized.
Zhe Zhang, Yuan Cao, Jing Du, Ying Zhang, Junxia Wang, Ying Yuan, Lianqing Sun   +6 more
wiley   +1 more source

Minimizing technical variation during sample preparation prior to label-free quantitative mass spectrometry [PDF]

, 2015
Sample preparation is the crucial starting point to obtain high-quality mass spectrometry data and can be divided into two main steps in a bottom-up proteomics approach: cell/tissue lysis with or without detergents and a(n) (in-solution) digest ...
De Sutter, Petra, Deforce, Dieter, Dhaenens, Maarten, Peelman, Luc, Scheerlinck, Ellen, Van Soom, Ann, Van Steendam, Katleen   +6 more
core   +2 more sources