Calorimetry of Some Trypsin-Trypsin Inhibitor Reactions
Journal of Biological Chemistry, 1972 The heats of reaction of trypsin with soybean (Kunitz), ovomucoid, and lima bean inhibitors have been measured at pH 5.0 and 10° and 25°. All the reactions were endothermic, with ΔH ranging from 8.6 Cal per mole for the lima bean inhibitor to 15.3 Cal per mole for the soybean inhibitor. Equilibrium constants for the association were calculated from the
Robert J. Baugh, C.G. Trowbridge
openaire +3 more sources
Insertion of the FeB cofactor in cNORs lacking metal inserting chaperones
FEBS Letters, EarlyView.Nitric oxide reductase is an enzyme found in the bacterial denitrification pathway. The NOR active site contains a non‐heme iron, often, but not always inserted with the assistance of chaperones. Here, we study the insertion of FeB in the subfamily of cNORs lacking chaperones and found a putative channel, conserved in the family, perhaps enabling the ...
Sofia Appelgren, Pia Ädelroth
wiley +1 more source
Weak self-interactions of globular proteins studied by small-angle X-ray scattering and structure-based modeling [PDF]
J. Phys. Chem. B 118, 10111-10119 (2014), 2014 We investigate protein-protein interactions in solution by small-angle X-ray scattering (SAXS) and theoretical modeling. The structure factor for solutions of bovine pancreatic trypsin inhibitor (BPTI), myoglobin (Mb), and intestinal fatty acid-binding protein (IFABP) is determined from SAXS measurements at multiple concentrations, from Monte Carlo ...
arxiv +1 more source
Activity of Enzyme Trypsin Immobilized onto Macroporous Poly(Epoxy-Acrylamide) Cryogel [PDF]
, 2014 Bovine pancreatic trypsin has been covalently immobilized onto monolithic polyacrylamide cryogels with epoxy functionality (Epoxy-MPAAGs). The covalent immobilization of trypsin onto Epoxy-MPAAGs was achieved through incorporation of ethylenediamine ...
Johnson, W, Makame, YMM
core +2 more sources
Label-free nanopore single-molecule measurement of trypsin activity.
ACS Sensors, 2016 Trypsin is the most important digestive enzyme produced in the pancreas, and is a useful biomarker for pancreatitis. In this work, a rapid and sensitive method for the quantitative determination of trypsin activity is developed by using a biological ...
Shuo Zhou +3 more
semanticscholar +1 more source
FEBS Letters, EarlyView.Venom peptides have shown promise in treating pain. Our study uses computer screening to identify a peptide that targets a sodium channel (NaV1.7) linked to chronic pain. We produced the peptide in the laboratory and refined its design, advancing the search for innovative pain therapies.
Gagan Sharma +8 more
wiley +1 more source
Characterization of ferrite nanoparticles for preparation of biocomposites
Beilstein Journal of Nanotechnology, 2017 Ferrite nanoparticles with nominal composition Me0.5Fe2.5O4 (Me = Co, Fe, Ni or Mn) have been successfully prepared by the wet chemical method. The obtained particles have a mean diameter of 11–16 ± 2 nm and were modified to improve their magnetic ...
Urszula Klekotka +3 more
doaj +1 more source
Structure and kinetics of chemically cross-linked protein gels from small-angle X-ray scattering [PDF]
Phys. Chem. Chem. Phys. 16, 4002-4011 (2014), 2013 Glutaraldehyde (GA) reacts with amino groups in proteins, forming intermolecular cross-links that, at sufficiently high protein concentration, can transform a protein solution into a gel. Although GA has been used as a cross-linking reagent for decades, neither the cross-linking chemistry nor the microstructure of the resulting protein gel have been ...
arxiv +1 more source
Optimisation of tennis string production from bovine intestine : a thesis presented in partial fulfilment of the requirements for the degree of Master in Technology at Massey University, Palmerston North, New Zealand [PDF]
, 1999 The collagen and elastin content of the beef thread samples ranged from between 47-70% and 1.2-2.5% respectively. Amino acid analysis showed that the collagen present was probably collagen Type I while the non-collagenous proteins predominantly were ...
Komolwattanachai, Kanda
core
Amino acid sequence elucidation of human acrosin-trypsin inhibitor (HUSI-II) reveals that Kazal-type proteinase inhibitors are structurally related to β-subunits of glycoprotein hormones [PDF]
, 1990 The amino acid sequence of the acrosin-trypsin inhibitor HUSI-II from human seminal plasma is presented which unequivocally identifies HUSI-II as being of Kazal-type.
Eulitz, Manfred +2 more
core +1 more source