Results 131 to 140 of about 18,157 (223)
Zhongguo shiyan zhenduanxue, 2019 目的为探讨mTOR信号通路在骨骼发育过程中的机制作用,建立骨骼发育相关的TSC1转基因小鼠,提供稳定的动物模型。方法取8周龄健康清洁级TSC1flox/flox小鼠分别与肢芽干细胞特异性重组酶(Prx-1-Cre)小鼠、软骨细胞特异性重组酶(Col2al-Cre)小鼠及成骨细胞特异性重组酶(Osx-Cre)小鼠进行杂交。将繁殖出的小鼠继续与TSC1flox/flox小鼠回交,并对其子代小鼠的基因型进行鉴定及mTOR活性检测。结果杂交后分别获得肢芽干细胞特异性TSC1敲除小鼠 ...
王红 +7 more
doaj
TSC1 Mosaicism Leading to Subependymal Giant Cell Astrocytoma but Not Tuberous Sclerosis Complex
, 2021 Adriana Hall +4 more
openalex +2 more sources
Zhongguo shiyan zhenduanxue目的基于Cre-loxp重组酶系统，构建破骨细胞特异性Tsc1基因条件性敲除（cKO）小鼠模型，为深入研究Tsc1基因在骨质疏松症及其他骨代谢性疾病中的分子机制提供可靠的实验动物模型。方法将CtskCre/+小鼠与Tsc1flox/flox小鼠进行交配与筛选，获得实验组（CtskCre/+/Tsc1flox/flox）及其同窝对照的野生型小鼠。采用碱裂解法提取小鼠基因组DNA，并通过PCR扩增及琼脂糖凝胶电泳进行基因型鉴定。提取总蛋白后采用Western blot技术在蛋白水平检测TSC1蛋白的表达情况。
张晟 +4 more
doaj
TSC1 Stabilizes TSC2 by Inhibiting the Interaction between TSC2 and the HERC1 Ubiquitin Ligase [PDF]
, 2006 Huira C. Kopera +6 more
openalex +1 more source
Tuberous sclerosis complex tumor suppressor 1 (TSC1); TSC2; mammalian target of rapamycin (mTOR; FRAP; RAFT1) [PDF]
, 2012 openalex +1 more source
Immunohistochemical Diagnostic Algorithm for Renal Cell Carcinoma with Fibromyomatous Stroma
Bezmiâlem ScienceBusra YAPRAK BAYRAK, Mahmut AKGUL
doaj +1 more source
Regulable neural progenitor-specific Tsc1 loss yields giant cells with organellar dysfunction in a model of tuberous sclerosis complex [PDF]
, 2011 June Goto +14 more
openalex +1 more source