Results 41 to 50 of about 31,308 (219)

Context-Specific Requirement of Forty-Four Two-Component Loci in Pseudomonas aeruginosa Swarming

iScience, 2019
Summary: Swarming in Pseudomonas aeruginosa is a coordinated movement of bacteria over semisolid surfaces (0.5%–0.7% agar). On soft agar, P. aeruginosa exhibits a dendritic swarm pattern, with multiple levels of branching.
Ameen M. Kollaran, Shubham Joge, Harshitha S. Kotian, Divakar Badal, Deep Prakash, Ayushi Mishra, Manoj Varma, Varsha Singh   +7 more
doaj   +1 more source

Recent Advances in Histidine Kinase-Targeted Antimicrobial Agents

Frontiers in Chemistry, 2022
The prevalence of antimicrobial-resistant pathogens significantly limited the number of effective antibiotics available clinically, which urgently requires new drug targets to screen, design, and develop novel antibacterial drugs.
Hongtong Chen, Chengqi Yu, Han Wu, Han Wu, Guoqing Li, Congran Li, Wei Hong, Xinyi Yang, Hao Wang, Hao Wang, Hao Wang, Xuefu You   +11 more
doaj   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Two-Component Signal Transduction Systems: A Major Strategy for Connecting Input Stimuli to Biofilm Formation

Frontiers in Microbiology, 2019
Biofilms are multicellular communities of microbes that are encased within an extracellular matrix. Environmental factors induce bacteria to form biofilm.
Cong Liu, Di Sun, Jingrong Zhu, Weijie Liu   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Role Of Two-Component Signal Transduction Systems In Antimicrobial Resistance Of Gram-Negative Pathogens

Postępy Mikrobiologii, 2020
Two-component signal transduction systems composed of histidine sensor kinase and response regulator are involved in adaptive response of pathogenic bacteria to environmental signals by regulating gene expression involved in many physiological processes,
Raczkowska Adrianna, Jaworska Karolina, Wyrożemski Łukasz, Brzostek Katarzyna   +3 more
doaj   +1 more source

Sex Representation in US Stroke Clinical Trials: A Decade of Trends and Challenges

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Stroke remains a major cause of disability and mortality in the US, with significant sex‐based disparities, and females remain underrepresented in stroke clinical trials. We aimed to examine sex representation in US‐based stroke clinical trials, identify trial characteristics associated with higher female enrollment (≥ 50%), and ...
Chaitali Dagli, Mudasir Andrabi, Tova Cohen, Pranali G. Patel, Tapasya Katta, Chen Lin   +5 more
wiley   +1 more source

Development of a Disease Activity Index for the Assessment of VEXAS Syndrome (VEXAS‐DAI)

Arthritis Care &Research, Accepted Article.
Objective VEXAS syndrome is characterized by a complex spectrum of inflammatory and hematologic manifestations. Clinical research to identify effective therapies is urgently needed but is hindered by the lack of validated outcome measures. A VEXAS‐specific disease activity index (DAI) is an essential tool for reliably capturing changes in disease ...
Kevin Byram, Heřman Mann, Danielle Hammond, Onima Chowdhury, Sinisa Savic, Yohei Kirino, Carmelo Gurnari, Maël Heiblig, Thibault Comont, Arsène Mekinian, Mrinal M. Patnaik, Lachelle D. Weeks, Gary Ho, Adam Al‐Hakim, Scott Goldberg, Marcela A. Ferrada, Sophie Georgin‐Lavialle, Peter C. Grayson, Emma M. Groarke, Bhavisha Patel, Megan Sullivan, Sarah A. Buckley, Bryan G. Harder, Raman Garcha, David B. Beck, Matthew J. Koster   +25 more
wiley   +1 more source

Factors Associated with Fibromyalgia Diagnosis amongst People Meeting Criteria: Results from UK Biobank

Arthritis Care &Research, Accepted Article.
Objective The diagnosis of fibromyalgia (FM) is challenging due to the absence of definitive biomarkers, numerous overlapping comorbidities and its reliance on patient‐reported symptoms. Discrepancies between diagnostic criteria and clinical practice imply the possibility of diagnostic biases, complicating timely and accurate identification. This study
Sung‐A Kim, Gary J. Macfarlane, Marcus J. Beasley   +2 more
wiley   +1 more source

Activation of the Staphylococcus aureus intramembrane sensing histidine kinase SaeS via intramembrane interaction with the bacterially encoded small protein ScrA

mBio
Two-component systems represent a fundamental sense-response mechanism in bacterial cells and often play important roles in critical processes such as quorum sensing, antibiotic tolerance, and virulence.
Marcus A. Wittekind, Ian R. Monk, Ryan W. Steere, Emily G. Sudnick, Donald Holzschu, Padraig Deighan, Timothy P. Stinear, Ronan K. Carroll   +7 more
doaj   +1 more source