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Phosphoproteome Analysis Using Two-Dimensional Electrophoresis Coupled with Chemical Dephosphorylation [PDF]

open access: yesFoods, 2022
Protein phosphorylation is a reversible post-translational modification (PTM) with major regulatory roles in many cellular processes. However, the analysis of phosphoproteins remains the most challenging barrier in the prevailing proteome research ...
Raquel Rodríguez-Vázquez   +2 more
doaj   +2 more sources

Two-dimensional electrophoresis protocols

open access: yesEuropean Journal of Histochemistry, 2010
It is worthy to buy this series of protocols on a classical well established technique, especially for those are newcomer users: I say this because today quite often the youngest colleagues are totally devoted to smashing techniques forgetting those ...
CarloAlberto Redi
doaj   +3 more sources

Preliminary Analysis of Taenia multiceps Metacestode Antigens by Two-Dimensional Electrophoresis [PDF]

open access: yesIranian Journal of Parasitology, 2014
Background: Taenia multiceps is a cestode parasite with its larval stage(metacestode), Coenurus cerebralis, mainly encysts in the central nervous system of sheep and other livestock causing cerebralis coenurosis.
Wen Hui Li   +8 more
doaj   +1 more source

Effect of Chitosan Composite Preservative on Proteome Changes of Grass Carp Muscle during Cold Storage [PDF]

open access: yesShipin Kexue, 2023
To explore the effect of a chitosan-based preservative solution (10 mg/mL chitosan + 5 mg/mL tea polyphenol + 2 000 U/mL lysozyme) on the change in the muscle proteome of grass carp during cold storage, differential proteomic analysis of fresh fish and ...
LI Jialei, CHEN Sai, TIAN Mingli, YU Jian, LIU Yongle, WANG Faxiang
doaj   +1 more source

A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163 [PDF]

open access: yesOpen Biology, 2014
Oenococcus oeni is the main lactic acid bacterium that carries out the malolactic fermentation in virtually all red wines and in some white and sparkling wines.
María de la Luz Mohedano   +6 more
doaj   +1 more source

Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones

open access: yesTecnología en Marcha, 2022
A classical strategy to analyse the protein content of a biological sample is the two-dimensional gel electrophoresis (2D-GE). This technique separates proteins by both isoelectric point and molecular weight, and images are taken for subsequent analyses.
José Arturo Molina-Mora   +3 more
doaj   +1 more source

Comparative proteomic analysis of plasma proteins in patients with age-related macular degeneration [PDF]

open access: yesInternational Journal of Ophthalmology, 2014
AIM: To find the significant altered proteins in age-related macular degeneration (AMD) patients as potential biomarkers of AMD.METHODS: A comparative analysis of the protein pattern of AMD patients versus healthy controls was performed by means of ...
Xin-Rong Xu   +6 more
doaj   +1 more source

Identification of proteins in sensitive and tolerant lines of sunflower (Helianthus annus L.) under water deficit

open access: yesActa Agriculturae Slovenica, 2021
The importance of examining environmental stresses and their role in predicting and evaluating the growth and yield of crops is very evident. Environmental stresses are the most important factor in reducing agricultural yields worldwide.
Babak MAGHSOUDI DAMAVANDI   +4 more
doaj   +1 more source

Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9

open access: yesVirology Journal, 2021
Background Both the highly pathogenic avian influenza (HPAI) H5N1 and low pathogenic avian influenza (LPAI) H9N2 viruses have been reported to cross species barriers to infect humans.
Yuting Yang   +7 more
doaj   +1 more source

Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis [PDF]

open access: yes, 2006
Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins.
Chevallet, Mireille   +3 more
core   +4 more sources

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